Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes in Nigerian patients with duodenal ulcer disease

Abstract

Distinct virulence factors of Helicobacter pylori have been associated with clinical outcome of the infection; however, considerable variations have been reported from different geographic regions. Data on genotypes of African H. pylori isolates are sparse. The aim of this study was to determine the prevalence of specific genotypes of H. pylori in Nigerian patients with duodenal ulcer and non-ulcer dyspepsia. H. pylori was cultured from endoscopic biopsies obtained from 41 Nigerian patients (19 with duodenal ulcer, 22 with non-ulcer dyspepsia). The vacA alleles, cagA and iceA genotypes were determined by PCR. The vacA s1,m1 and s1,m2 genotypes were found in 26.3% and 22.7%, and in 73.7% and 72.7% of H. pylori isolates from patients with duodenal ulcer and non-ulcer dyspepsia, respectively. The iceA1 genotype was present in 94.7% and 86.4% of isolates from duodenal ulcer and non-ulcer dyspepsia patients, respectively. cagA infection was found predominantly (>90%) in Nigerian H. pylori isolates irrespective of the clinical diagnosis. In conclusion, vacA s1,m2, iceA1 and cagA are common genotypes of H. pylori isolated from Nigerian patients. As in several other developing countries there seems to be no association between these genotypes and duodenal ulcer disease.

Prevalence of Helicobacter pylori vacA and cagA Genotypes in Ethiopian Dyspeptic Patients

“…The pattern of vacA alleles in this study is in agreement with those reported in other studies (3,8,16,18,20). However, the frequency of vacA s1/m1 allelic type in this study is higher than figures reported from The Netherlands (36%), Hong Kong (26 to 31%), and Nigeria (24%), but lower than figures reported from Brazil (80%) and Korea (78%) (3,11,16,20,21). In the present investigation, the rare vacA s2/m1 allele was detected in 4 (2%) of the 246 gastric biopsy specimens examined, also reported in studies in South Africa and Chile (12,13).…”

“…The vacA genotypes s1/m1, s1/m2, s2/m2, and s2/m1 were found in 48, 28, 9, and 2% of the specimens, respectively (Table 1), whereas, 15 biopsy specimens (6%) were incomplete and thus did not yield a detectable PCR product for the vacA s or m regions. The pattern of vacA alleles in this study is in agreement with those reported in other studies (3,8,16,18,20). However, the frequency of vacA s1/m1 allelic type in this study is higher than figures reported from The Netherlands (36%), Hong Kong (26 to 31%), and Nigeria (24%), but lower than figures reported from Brazil (80%) and Korea (78%) (3,11,16,20,21).…”

“…In addition, no statistical differences in the frequency of detection of the different vacA allelic types from gastric biopsy specimens and clinical isolates were found (P Ͼ 0.05). The prevalence of cagA positive H. pylori strains varies from one geographic region to another, e.g., 38% in Chile, 48% in Sri Lanka, 67% in The Netherlands, 81% in the United Sates, 90% in Hong Kong, 97% in Korea, 93% in Nigeria and 94% in Brazil (3,8,11,13,16,18,20,21). Correlation of histopathology results with vacA and cagA genotypes showed that vacA and cagA positive strains were detected to a higher degree in patients with chronic active gastritis (71%) compared with patients with other histopathological findings (29%) (P Ͻ 0.05) ( (4) (15) a vacA gene was incomplete (Inc) to yield a detectable PCR product for the vacA s or m region.…”

The Prevalence of <italic>Helicobacter pylori</italic> <italic>babA2, iceA1 </italic> and <italic>iceA2 </italic> Genes and Their Association with Clinical Outcomes in Patients with Chronic Gastritis, Ulcerative Diseases and Non-Ulcer Dyspepsia in South East of Iran

“…Likewise Van doorn et al announced that isolates with iceA1 are more leading to ulcerative diseases (14). In contrast with Smith et al study that detected the iceA gene in all of the H.pylori isolates from Nigerian patients; we detected the iceA1 gene in only 22.2% and the iceA2 gene in 54% of our cases, but in accordance to our study they detect no association between the iceA1 allele and peptic ulcer disease (22).…”

Prevalence and Genotypes of Helicobacter pylori in Gastric Biopsy Specimens from Patients with Gastroduodenal Pathologies in the Cukurova Region of Turkey

“…The prevalence of type I H. pylori in patients with DU was observed to be significantly higher than in patients with G/GU (94.3% and 78.6%, respectively; P ϭ 0.004) ( Table 2). In a great amount of research, the prevalence of type I H. pylori was reported to vary among different countries, regions, and patient groups, and it was reported as 68% in England (21), 78 to 80% in Turkey (5,15), 80% in Belgium (11), 82% in Brazil (1), 82% in Japan (17), 93% in Nigeria (16), and 97% in China (24). The majority of those studies (1,5,11,17,21) indicated that in patients with DU, the cagA positivity rate is relatively higher than in patients with gastritis or GU and ranges from 80 to 100%.…”