Prevalence of human papillomavirus infections among heterosexual men and women with multiple sexual partners

Abstract

A prospective study of 65 men and 111 women with multiple heterosexual partners was designed to assess the prevalence and potential risk factors of genital human papillomavirus (HPV) infections. In addition, the HPV reservoir in genital, rectal, and oral mucosa was examined. The specimens for the detection of HPV DNA were taken from different sites such as the urethra and coronal sulcus (men), cervix and labia minora (women), anus, rectum, tongue, and buccal mucosa (both men and women). Women underwent speculum examination and colposcopic evaluation of the anogenital region, and a smear for routine cytological classification was also taken. In men, the anogenital region was examined clinically and colposcopically. The polymerase chain reaction (PCR) was used for the detection of HPV types 6/11, 16, 18, and 33. A high prevalence of HPV infection at one or more sites was detected, in 32% of the male and in 23% of the female participants. Seventeen percent of the male distal urethral specimens were positive for HPV DNA. From the female cervical specimens 14% were found positive. Ten proctal specimens (five men and five women) were positive for HPV DNA without any discernible lesion. The persons from whom these samples were taken denied anal insertive intercourse. No oral manifestation of HPV infection was detected. In both men and women a difference between HPV DNA-positive and -negative persons was not found in relation to known risk factors associated HPV infection.(ABSTRACT TRUNCATED AT 250 WORDS)

Identification of human papillomavirus seroconversions

“…Of these, 110 women and 48 men could be followed until January 1992. These patients were included in this study (for a further description of the cohort, see van Doornum et al, 1992).…”

“…At each visit, specimens were taken for HPV DNA detection with the PCR assay, using type-specific primers for HPV-6/11, -16, -18 and -33 as described (van Doornum et al, 1992). Samples from the males were taken from the mouth, anus, rectum, coronal sulcus and urethra.…”

“…se suggested that a high proportion of infections are transient, i.e. DNA of a specific HPV type is frequently only detected on a single occasion (van Doornum et al, 1992;Rosenfeld et al, 1992). Several epitopes reactive with antibodies preferentially found in sera of patients with cervical intraepithelial neoplasia (CIN) or cervical cancer have been defined for the E2, E6, E7 and L1 proteins ofHPV-16 (J.…”

Detecção de HPV na mucosa oral e genital pela técnica PCR em mulheres com diagnóstico histopatológico positivo para HPV genital

“…8,9 The samples of the mouth were PCR-negative in all 30 patients of this study. In other studyes [22][23][24][25][26] that used the same technique, the positivity rate ranged from 0 to 50%. We concluded that the presence of genital HPV infection does not appear to be a predisposing factor for oral HPV infection.…”

“…21 Many researchers have studied oral and genital HPV to discover whether genital infection by this virus could lead to infection elsewhere, such as in the mouth. [22][23][24][25][26] Van Doornum et al 22 (1992) applied the PCR to study oral and genital HPV in 65 males and 111 females. Examination of the mouth consisted of unaided visual inspection and scrapes for further study.…”