Prevalence of Mycobacterium bovis infection in traditionally managed cattle at the wildlife-livestock interface in South Africa in the absence of control measures

Sichewo, Petronillah Rudo; Etter, Éric; Michel, Anita Luise

doi:10.1007/s11259-019-09756-w

Cited by 18 publications

(25 citation statements)

References 35 publications

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“…This has been established in the context of bTB in cattle with reference to numerous genotypes displayed by M. bovis isolates from developed countries where bTB prevalence is low due to successful national control programs [40]. In our study setting where a high bTB was found [32], the SBO130 was dominant in both cattle and wildlife, indicative of a poor efficacy of current control measures in this communal area.…”

Section: Discussionmentioning

confidence: 83%

“…The cattle samples were obtained from animals that were bTB tested during a cross-sectional study that was conducted at 4 dip tanks (Nibela, Nkomo, Mpempe, Masakeni) in Big 5 False Bay Municipality, in uMkhanyakude district, northern KwaZulu-Natal province, South Africa. Bovine TB testing of cattle was previously carried out in September 2016 and March 2017 as part of a One Health investigation into the epidemiology of bTB at the wildlife/livestock/human interface [32]. Additional cattle samples were collected from farms and other dip tanks in the province during bTB outbreaks.…”

Section: Study Areamentioning

confidence: 99%

“…Wildlife samples were collected from the surrounding game reserves that include HiP, Munyawana and Mkhuze. The map of the study area has been previously described by [32].…”

Section: Study Areamentioning

confidence: 99%

“…In June and July 2017, 30 milk samples were collected from interferon gamma positive animals that belonged to the 4 dip tanks were bTB testing was previously carried out in September 2016 and March 2017 [32]. An average of 25 ml of milk was collected into 50 ml centrifuge tubes from all lactating animals.…”

Section: Sample Collection and Processing Milk Nasal And Tissue Sampmentioning

confidence: 99%

“…The study area is a communal farmland that is surrounded by public and private game reserves where bTB has been previously reported in wildlife, such as the Hluhluwe iMfolozi Park (HiP), Munyawana and Mkhuze game reserves [22,31]. Recently bTB was detected in traditionally farmed cattle in this wildlife/livestock interface [32]. Currently, there is inadequate information on the inter species transmission of bTB between the game reserves and neighbouring communities.…”

Section: Introductionmentioning

confidence: 99%

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Tracing cross species transmission of Mycobacterium bovis at the wildlife/livestock interface in South Africa

et al. 2020

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Background: Bovine tuberculosis (bTB) affects cattle and wildlife in South Africa with the African buffalo (Syncerus caffer) as the principal maintenance host. The presence of a wildlife maintenance host at the wildlife/livestock interface acting as spill-over host makes it much more challenging to control and eradicate bTB in cattle. Spoligotyping and mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRU-VNTR) genotyping methods were performed to investigate the genetic diversity of Mycobacterium bovis (M. bovis) isolates from cattle and wildlife, their distribution and transmission at the wildlife/livestock interface in northern Kwa-Zulu Natal (KZN), South Africa.Results: SB0130 was identified as the dominant spoligotype pattern at this wildlife/livestock interface, while VNTR typing revealed a total of 29 VNTR profiles (strains) in the KZN province signifying high genetic variability. The detection of 5 VNTR profiles shared between cattle and buffalo suggests M. bovis transmission between species. MIRU-VNTR confirmed co-infection in one cow with three strains of M. bovis that differed at a single locus, with 2 being shared with buffalo, implying pathogen introduction from most probably unrelated wildlife sources. Conclusion:Our findings highlight inter and intra species transmission of bTB at the wildlife/livestock interface and the need for the implementation of adequate bTB control measures to mitigate the spread of the pathogen responsible for economic losses and a public health threat.

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Section: Discussionmentioning

confidence: 83%

Section: Study Areamentioning

confidence: 99%

“…Wildlife samples were collected from the surrounding game reserves that include HiP, Munyawana and Mkhuze. The map of the study area has been previously described by [32].…”

Section: Study Areamentioning

confidence: 99%

Section: Sample Collection and Processing Milk Nasal And Tissue Sampmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tracing cross species transmission of Mycobacterium bovis at the wildlife/livestock interface in South Africa

et al. 2020

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Application of the gamma‐interferon assay to determine the prevalence of bovine tuberculosis in slaughter livestock at abattoirs in Gauteng, South Africa

Mareledwane

Adesiyun

Thompson

et al. 2021

Veterinary Medicine & Sci

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Background Bovine tuberculosis (bTB) is a zoonotic disease with great economic impact estimated at billions of dollars annually worldwide. Meat inspection represents a long‐standing form of disease surveillance that serves both food safety and animal health. The objective of this study was to determine the prevalence of bTB in livestock at abattoirs using a cell‐mediated immune (CMI) assay, the gamma interferon (IFN‐γ) assay. This cross‐sectional study was conducted at selected abattoirs (low‐throughput, high‐throughput and rural/informal) in Gauteng province, where animals were also subjected to routine meat inspection. Results A total of 410 fresh blood samples were collected from slaughter livestock (369 cattle and 41 sheep) from 15 abattoirs, and analysed using Bovigam® test kit with bovine, avian and Fortuitum purified protein derivatives (PPD) as blood stimulating antigens. The estimated prevalence of bTB in cattle was 4.4% (95% CI: 2.4%–7.3%). The prevalence of bTB in cattle varied between abattoirs (p = .005), ranging from 0% to 23%; however, there were no significant differences among genders, breeds, municipality, districts, origins of animals (feedlot, auction or farm) or throughput of abattoirs. The prevalence of avian reactors was 6.0% (95% CI: 3.6%–9.2%) in cattle, varying between abattoirs (p = .004) and ranging from 0% to 20.7%. None of the sheep with valid test results was positive for bTB and none was avian reactors (95% CI: 0%–15%). Conclusion The detection of bTB reactor cattle in our study clearly shows the limitation of disease surveillance using a meat inspection approach, as all the 410 slaughter animals sampled had passed visual abattoir inspection and been classified as bTB‐free. Our findings therefore emphasize the risk of zoonotic transmission of bTB to abattoir workers and potential food safety hazard to consumers. Furthermore, our study highlights the potential for the use of the IFN‐γ assay to reduce this risk.

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Presence of pathogenic microorganisms in fermented foods

Oyedeji

Green

Jeff-Agboola

et al. 2023

Indigenous Fermented Foods for the Tropics

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Prevalence of Mycobacterium bovis infection in traditionally managed cattle at the wildlife-livestock interface in South Africa in the absence of control measures

Cited by 18 publications

References 35 publications

Tracing cross species transmission of Mycobacterium bovis at the wildlife/livestock interface in South Africa

Tracing cross species transmission of Mycobacterium bovis at the wildlife/livestock interface in South Africa

Application of the gamma‐interferon assay to determine the prevalence of bovine tuberculosis in slaughter livestock at abattoirs in Gauteng, South Africa

Presence of pathogenic microorganisms in fermented foods

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