Prevalence of porcine epidemic diarrhoea virus and transmissible gastroenteritis virus infection in Korean pigs

Chae, Chanhee; Kim, O; Choi, Changsun; Min, Kyoung-Bok; Cho, W S; Kim, J; Tai, Joo Ho

doi:10.1136/vr.147.21.606

Cited by 82 publications

(58 citation statements)

References 12 publications

(20 reference statements)

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“…Most of the affected farms reported that the disease first occurred in farrowing barns; 100% of newborn piglets were subsequently lost. Between August 1997 and July 1999, 50.4% of 1,258 enteric cases across 5 Korean provinces were diagnosed as PED [9]; further, a Korean abattoir serosurvey found PEDV seroprevalences of 17.6-79% (mean of 45%) in samples from 469 pigs from seven provinces. Cumulatively, these results suggest that the virus had become endemic in some areas [10] ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

2012

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The porcine epidemic diarrhoea virus (PEDV), a member of the Coronaviridae family, causes acute diarrhoea and dehydration in pigs. Although it was first identified in Europe, it has become increasingly problematic in many Asian countries, including Korea, China, Japan, the Philippines, and Thailand. The economic impacts of the PEDV are substantial, given that it results in significant morbidity and mortality in neonatal piglets and is associated with increased costs related to vaccination and disinfection. Recently, progress has been made in understanding the molecular epidemiology of PEDV, thereby leading to the development of new vaccines. In the current review, we first describe the molecular and genetic characteristics of the PEDV. Then we discuss its molecular epidemiology and diagnosis, what vaccines are available, and how PEDV can be treated.

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Section: Introductionmentioning

confidence: 99%

Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

2012

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“…At present, PED has been a major concern in the swine industry, particularly, in the Asia and Europe, resulting in large economic losses [2][3][4]. The causative agent of PED is porcine epidemic diarrhea virus (PEDV), an enveloped and single-stranded RNA virus that belongs to the family Coronaviridae [5].…”

Section: Introductionmentioning

confidence: 99%

Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus

Ren

2011

Virus Genes

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In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed for detection of porcine epidemic diarrhea virus (PEDV). Six primers were designed to amplify the nucleocapsid (N) gene of PEDV. The optimization, sensitivity, and specificity of the RT-LAMP were investigated. The results showed that the optimal reaction condition for RT-LAMP amplifying PEDV N gene was achieved at 63°C for 50 min. The RT-LAMP assay was more sensitive than gel-based RT-PCR and enzyme-linked immunosorbent assay. It was capable of detecting PEDV from clinical samples and differentiating PEDV from Porcine transmissible gastroenteritis virus, Porcine rotavirus, Porcine pseudorabies virus, Porcine reproductive and respiratory syndrome virus, and Avian infectious bronchitis virus.

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“…PEDV has been classified as a member of the Coronaviridae family [1,2]. PED was first detected in Belgium [3] and the UK [4] in 1978, and outbreaks of this disease have been reported in many pig farming countries, leading to severe economic losses in Canada [5], Europe [6,7], and Asia, including Japan [8], China [9], Korea [10], and more recently, Thailand [11]. The complete sequence of the entire genome of the strain CV777 was found to be 28,033 nucleotides in length, after excluding the poly(A) tail [12,13].…”

Section: Introductionmentioning

confidence: 99%

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

2010

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Transgenic plants have been used as a safe and economic expression system for the production of edible vaccines. A synthetic cholera toxin B subunit gene (CTB) was fused with a synthetic neutralizing epitope gene of the porcine epidemic diarrhea virus (sCTB-sCOE), and the sCTB-sCOE fusion gene was introduced into a plant expression vector under the control of the ubiquitin promoter. This plant expression vector was transformed into lettuce (Lactuca sativa L.) using the Agrobacterium-mediated transformation method. Stable integration and transcriptional expression of the sCTB-sCOE fusion gene was confirmed using genomic DNA PCR analysis and northern blot analysis, respectively. The results of western blot analysis with anti-cholera toxin and anti-COE antibody showed the synthesis and assembly of CTB-COE fusion protein into oligomeric structures with pentameric sizing. The biological activity of CTB-COE fusion protein to its receptor, G(M1)-ganglioside, in transgenic plants was confirmed via G(M1)-ELISA with anti-cholera toxin and anti-COE antibody. Based on G(M1)-ELISA, the expression level of CTB-COE fusion proteins reached 0.0065% of the total soluble protein in transgenic lettuce leaf tissues. Transgenic lettuce successfully expressing CTB-COE fusion protein will be tested to induce efficient immune responses against porcine epidemic diarrhea virus infection by administration with raw material.

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Prevalence of porcine epidemic diarrhoea virus and transmissible gastroenteritis virus infection in Korean pigs

Cited by 82 publications

References 12 publications

Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

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