Prevalence Survey of Bovine Brucellosis in Apparently Healthy Dairy Animals in Karachi, Pakistan

Abstract

| Brucellosis is one of the major zoonosis diseases that are still prevalent throughout the world. The present modified cross-sectional study aims to determine the prevalence of brucellosis in apparently healthy dairy cattle/buffaloes in Karachi, Pakistan. A total of 100 farms at different localities of Karachi were visited and milk and blood samples (n=200 each) were collected. These were analysed by Rose Bengal Plate Test (RBPT), complement fixation test (CFT) and milk ring test (MRT). Results indicated a 17.5%, 12.5% seropositive samples by RBPT and CFT, respectively. While, 14% were recorded in the milk samples analysed by MRT. In different areas (towns) prevalence percentage varied highly, ranging from 5.88% to 25%. From these results it could be concluded that brucellosis is present in the apparently healthy dairy cattle and buffaloes of Karachi, however, its prevalence is low comparing with small cities of Pakistan. Furthermore, disease prevalence is diverse in different areas of Karachi. These results emphasize the need for a better monitoring of the disease by government policies or local veterinarians to overcome the disease prevalence.

Seroprevalence of Brucellosis in Holstein-Friesian and Indigenous Cattle Breeds of Sindh Province, Pakistan

“…Likewise, in a recent study by our laboratory, regarding prevalence of bovine brucellosis, it was found that Brucella abortus specific antibodies have higher ratio in buffalo (47/100) than cattle (31/100) (Soomro et al, 2014). We have also documented the geographical variations in our previous studies (Kamboh et al, 2007;Durrani et al, 2015) for prevalence of bovine brucellosis that might be of nutritional or managemental origin.…”

The Prevalence of Brucellosis in Kundhi Buffaloes in District Hyderabad, Pakistan

“…Like, a previous study conducted in Hyderabad indicated the 47.19% prevalence of B. abortus in buffaloes (Soomro et al, 2014), however the number of samples analyzed in the previous study were very little (n=89) as compared to our study. Similarly, another survey done in Karachi indicated the 14.00% prevalence of B. abortus antibodies in the milk of bovine population (Durrani et al, 2015). These results of high prevalence (14 to 47.19%) recorded previously might be due to the breed differences as our study was only on Kundhi buffaloes, however other researchers have study on mixed breed of buffaloes and cattle.…”

“…Brucellosis is also called Bang disease, which is a zoonotic disease throughout the world (Durrani et al, 2015), that is a common transmittable bacterial disease of livestock and have great economic importance worldwide including Pakistan (Munir et al, 2010). Brucellosis is caused by an aerobic gram-negative bacteria of the genus Brucella.…”

Isolation of Mycobacterium bovis from Milk and Nasal Discharge Samples of Cattle from Hyderabad and Tando Allahyar Districts

“…For nasal secretions, sterilized swabs were used, whereas for milk, sterilized bijoux bottles were used to collect 5ml milk from each lactating cattle. Before collection, the teats were cleaned with antiseptic agent and first few drops of milk were discarded (Durrani et al, 2015). Data regarding epidemiological risk factors such as sex, age, breed, type of farming, parity, pregnancy status, stage of lactation and milk production was also collected in pre-designed questionnaire.…”