Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate)

Kim, Hye Sung; Chen, Junyu; Wu, Ling-Ling; Wu, Jihua; Xiang, Hua; Leong, Kam W.; Han, Jing

doi:10.1177/2041731420949332

Cited by 26 publications

(20 citation statements)

References 54 publications

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“… 40 To reduce excessive scarring in wound healing, some researchers used polymer meshes as a wound dressing to treat wound. 41 In this study, we added a plastic clip between the 3D-printed PLCL scaffold with/without MFUS and the wound edge skin to block the upper layer skin cell migration. It is well known that skin wounds generally heal by scarring, a fibrotic process mediated by the Engrailed-1 (En1) fibroblast lineage.…”

Section: Discussionmentioning

confidence: 99%

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Chang

Sun

et al. 2022

J Tissue Eng

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Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing.

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Section: Discussionmentioning

confidence: 99%

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Chang

Sun

et al. 2022

J Tissue Eng

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“… 110 The degradation rate of PCL is lower than other materials (such as PGA, PLGA, and Poly L-lactic acid (PLLA)) 111 ; therefore, PCL nanofibers provide a long-term mechanical support in defective tissue. To reduce excessive scarring in wound healing, Kim et al 112 fabricated electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as a wound dressing. And the results demonstrated that electrospun nanofibrous PHBV meshes mitigate excessive scar formation by regulating myofibroblast formation, showing their promise for use as wound dressings.…”

Section: The Essential Elements For Skin Bioprintingmentioning

confidence: 99%

3D bioprinting for skin tissue engineering: Current status and perspectives

et al. 2021

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Skin and skin appendages are vulnerable to injury, requiring rapidly reliable regeneration methods. In recent years, 3D bioprinting has shown potential for wound repair and regeneration. 3D bioprinting can be customized for skin shape with cells and other materials distributed precisely, achieving rapid and reliable production of bionic skin substitutes, therefore, meeting clinical and industrial requirements. Additionally, it has excellent performance with high resolution, flexibility, reproducibility, and high throughput, showing great potential for the fabrication of tissue-engineered skin. This review introduces the common techniques of 3D bioprinting and their application in skin tissue engineering, focusing on the latest research progress in skin appendages (hair follicles and sweat glands) and vascularization, and summarizes current challenges and future development of 3D skin printing.

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“…The utilization of BGs as a dental material began in the mid-1980s, when BGs were successfully used to preserve the alveolar bone of edentulous patients [15]. Furthermore, BGs have been developed into bioactive glass nanoparticles (BGns), which have a high surface area, bioactivity, and antibacterial effect [16,17]. Many researchers have attempted to mix BGns with various materials and have reported improvements in mechanical properties, bioactivity, and mineralization capacities [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles

et al. 2022

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Silver diamine fluoride (SDF) is an outstanding dental material for arresting and preventing caries, but some drawbacks, such as high flowability due to low viscosity and cytotoxicity to the pulp, have been reported. To overcome these problems, copper-doped bioactive glass nanoparticles (CuBGns) were combined with SDF. After synthesis, CuBGns were examined by physical analysis and added in SDF at different weight/volume% (SDF@CuBGn). After assessing physical properties (viscosity and flowability) of SDF@CuBGn, physicochemical properties (morphology before and after simulated body fluid (SBF) immersion and ion release) of SDF@CuBGn-applied hydroxyapatite (HA) discs were evaluated. Biological properties were further evaluated by cytotoxicity test to pulp stem cells and antibacterial effect on cariogenic organisms (Streptococcus mutans and Staphylococcus aureus). Combining CuBGns in SDF increased the viscosity up to 3 times while lowering the flowability. More CuBGns and functional elements in SDF (Ag and F) were deposited on the HA substrate, even after SBF immersion test for 14 days, and they showed higher Cu, Ca, and Si release without changing F and Ag release. Cell viability test suggested lower cytotoxicity in SDF@CuBGn-applied HA, while CuBGns in SDF boosted antibacterial effect against S. aureus, ~27% in diameter of agar diffusion test. In conclusion, the addition of CuBGn to SDF enhances viscosity, Ag and F deposition, and antibacterial effects while reducing cell toxicity, highlighting the role of bioactive CuBGns for regulating physical and biological effects of dental materials.

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Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Cited by 26 publications

References 54 publications

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

3D bioprinting for skin tissue engineering: Current status and perspectives

Characterization of Physical and Biological Properties of a Caries-Arresting Liquid Containing Copper Doped Bioglass Nanoparticles

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