Prevention of rt-PA induced blood–brain barrier component degradation by the poly(ADP-ribose)polymerase inhibitor PJ34 after ischemic stroke in mice

Protection of the brain following cerebral ischemia through the attenuation of PARP-1-induced neurovascular unit damage in rats

“…In the present study, PARP-1 inhibition produced significant neuroprotection by reducing the infarct size and edema volume and attenuating the damage to the NVU after cerebral ischemia. These results are supported by previous studies in which PARP-1 inhibitors or gene deletion attenuated neurological damage in experimental models of cerebral ischemia (Hamby et al, 2007;Matsuura et al, 2011;Shimizu et al, 2013;Singh et al, 2014;Teng et al, 2013;Yap et al, 2008). Neurological impairments can be alleviated by PARP-1 inhibition, indicating that PARP-1 may be a key cofactor in NVU disruptions leading to cerebral injury (Chiarugi, 2005;Kauppinen et al, 2009;Matsuura et al, 2011;Moroni, 2008).…”

Structural Basis for the Identification of the N-Terminal Domain of Coronavirus Nucleocapsid Protein as an Antiviral Target

“…32 This compound also exhibited neuroprotective effects in both in vivo and in vitro stroke models. 33 To determine whether PJ34 hindered the HCoV-OC43 replication by interfering with the binding of the N protein to RNA, we used SPR to determine the effect of compound PJ34 on the RNA-binding affinity toward HCoV-OC43 N protein. In the presence of PJ34 under saturation conditions, RNA affinity for HCoV-OC43 NP decreased with decreasing resonance unit (RU) values for PJ34 (Figure 3C).…”

Retinoic acid ameliorates blood–brain barrier disruption following ischemic stroke in rats

“…Studies have reported that administration of tPA after ischemia aggravated the degradation of ZO-1, Claudin-5 and VE-cadherin [17]. Therefore, we investigated the effect of RA treatment after the onset of MCAO on the tPA-aggravated decrease of ZO-1 and VEcadherin 24 h after treatment using Western blot analysis and immunohistochemical staining.…”