Prevention of Tissue Adhesion by a Spontaneously Formed Phospholipid Polymer Hydrogel

Abstract

We investigated phospholipid polymer hydrogels containing Fe3+ ions (PMA/PMB/Fe hydrogel) for their use as antiadhesive materials in the healing tissues. These hydrogels were prepared from the aqueous solutions of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-comethacrylic acid) (PMA) and poly(MPC-co-n-butyl methacrylate) (PMB). The PMA/PMB hydrogel is formed by the intermolecular interactions between PMA and PMB, and it reversibly dissociates under physiological conditions. The addition of Fe3+ ions could control the gelation time and the dissociation time. Mechanical properties such as the gelation time and viscoelastic properties can be controlled by the FeCl3 concentration. With regard to biocompatibility, no evidence of inflammation was observed in vivo. Therefore, the PMA/PMB/Fe hydrogel has a potential to be used as an antiadhesive material.

The prevention of peritendinous adhesions by a phospholipid polymer hydrogel formed in situ by spontaneous intermolecular interactions

“…2). The dissociation was prevented dependently on the Fe 3þ concentrations, confirming that Fe 3þ increased the density of crosslinking and the stability of hydrogel, as reported previously [26].…”

“…2). This may be because Fe 3þ can regulate the crosslinking between PMA and PMB via hydrogen bonding and electrostatic interaction, as previously reported [26]. Further in vitro and in vivo assays determined the optimal Fe 3þ concentration for the tendon healing to be 0.05 M (Figs.…”

“…The extracted hydrogel was slowly inserted into both sides of a vibration blade. Immediately after the insertion, the blade was set in motion (vibrational amplitude: 200 mm; frequency: 20 Hz) and the elastic modulus and viscous modulus were recorded [26]. The elastic modulus is related to the energy stored in the sample to resist deformation and is a measure of the strength of the gel.…”

“…Since the Fe 3þ concentration in PMB is known to control crosslinking between PMA and PMB [26], we initially sought to determine the concentration required to prepare MPC polymer hydrogel with optimal viscoelasticity. The hydrogel was successfully formed immediately after mixing PMA and PMB, regardless of the Fe 3þ concentration (0-0.5 M) in PMB.…”

Bioadhesion of Polyion Complex (PlC) Hydrogels Composed of Amphiphilic Phospholipid Polymers

“…Moreover, introduction of multivalent cationic ions leads to another crosslinking formation between the carboxylate anions and the cations, and enables control of the dissociation time. Thus, the MPC polymer 595 hydrogel is expected to be a promising soft biomaterial for various applications such as an antiadhesive material for the healing process after a physical surgery [5].…”