Prevention of Vitrification Aßociated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp.

Shetty, Kalidas; Curtis, Otis F.; Levin, Robert E.; Witkowsky, R.; Ang, Woraluk

doi:10.1016/s0176-1617(11)82181-4

Cited by 316 publications

(189 citation statements)

References 24 publications

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“…The phenolic contents of C. japonica were determined by the method described by Shetty et al [11]. Each 1 ml of the algal extract was mixed with 1 ml of 95% EtOH, 5 ml of distilled water, and 0.5 ml of 50% Folin-Ciocalteu reagent (Sigma Chemical, St. Louis, MO).…”

Section: Determination Of Phenolic and Carbohydrate Contentsmentioning

confidence: 99%

Investigation of the Antiasthmatic Properties of Ethanol Extract of <i>Callophyllis japonica</i> in Mice

Park¹,

Lee²,

Chun³

et al. 2014

Trop. J. Pharm Res

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Section: Determination Of Phenolic and Carbohydrate Contentsmentioning

confidence: 99%

Investigation of the Antiasthmatic Properties of Ethanol Extract of <i>Callophyllis japonica</i> in Mice

Park¹,

Lee²,

Chun³

et al. 2014

Trop. J. Pharm Res

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“…For total phenolics and flavonoids, roots were homogenised in 95% ethanol (1 mL) and kept in the freezer for 48-72 h. The sample was then centrifuged at 19,000 x g for 20 min and measured following Shetty et al [35] and Zhuang et al [36], respectively. Fresh supernatant was used for flavonoids contents determination.…”

Section: Determination Of Total Phenolics Flavonoids Ascorbic Acidmentioning

confidence: 99%

Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures

2007

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Abstract:To investigate the enzyme variations responsible for the synthesis of phenolics, 40 day-old adventitious roots of Panax ginseng were treated with 200 µM methyl jasmonate (MJ) or salicylic acid (SA) in a 5 L bioreactor suspension culture (working volume 4 L). Both treatments caused an increase in the carbonyl and hydrogen peroxide (H 2 O 2 ) contents, although the levels were lower in SA treated roots. Total phenolic, flavonoid, ascorbic acid, non-protein thiol (NPSH) and cysteine contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reducing activity were increased by MJ and SA. Fresh weight (FW) and dry weight (DW) decreased significantly after 9 days of exposure to SA and MJ. The highest total phenolics (62%), DPPH activity (40%), flavonoids (88%), ascorbic acid (55%), NPSH (33%), and cysteine (62%) contents compared to control were obtained after 9 days in SA treated roots. The activities of glucose 6-phosphate dehydrogenase, phenylalanine ammonia lyase, substrate specific peroxidases (caffeic acid peroxidase, quercetin peroxidase and ferulic acid peroxidase) were higher in MJ treated roots than the SA treated ones. Increased shikimate dehydrogenase, chlorogenic acid peroxidase and β-glucosidase activities and proline content were observed in SA treated roots than in MJ ones. Cinnamyl alcohol Molecules 2007, 12 608 dehydrogenase activity remained unaffected by both MJ and SA. These results strongly indicate that MJ and SA induce the accumulation of phenolic compounds in ginseng root by altering the phenolic synthesis enzymes.

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“…Diverse biological actions, such as anti-inflammatory, anticarcinogenic and anti-atherosclerotic functions are considered part of these activities (30). Hence, phenolic and flavonoid compounds are the usual components analyzed when measuring antioxidant activity.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Free Radical Scavenging Activity of Different Fractions from Hawthorn Fruit

Park¹,

Li²,

Hu³

et al. 2010

JFN

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Hawthorn fruit is a conventional medicine used in treating cardiovascular diseases. Its therapeutic effects may relate to its antioxidant compounds. In this study, we evaluated the antioxidant activity of CH 2 Cl 2 , EtOAc, n-butanol and water fractions from 70% methanolic hawthorn fruit extract by total phenolic and flavonoid contents, total antioxidant activity, DPPH free radical scavenging activity, superoxide radical scavenging activity, reducing power assay, lipid peroxidation inhibitory activity and protective effect against hydroxyl-radical-induced DNA damage. Results showed that the EtOAc fraction contained significantly greater antioxidant activities than other fractions, which suggests that the potent EtOAc fraction should be used for further studies to identify the antioxidant compounds.

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Prevention of Vitrification Aßociated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp.

Cited by 316 publications

References 24 publications

Investigation of the Antiasthmatic Properties of Ethanol Extract of <i>Callophyllis japonica</i> in Mice

Investigation of the Antiasthmatic Properties of Ethanol Extract of <i>Callophyllis japonica</i> in Mice

Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures

Antioxidant and Free Radical Scavenging Activity of Different Fractions from Hawthorn Fruit

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