Primordial germ cell–like cells derived from canine adipose mesenchymal stem cells

Wei, Yudong; Fang, Jia You; Cai, Shaohui; Lv, Changrong; Zhang, Shiqiang; Hua, Jinlian

doi:10.1111/cpr.12271

Cited by 45 publications

(44 citation statements)

References 40 publications

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“…A previous study with human ovarian MSC also demonstrated the ability of those cells to differentiate into mesenchymal, ectodermal and endodermal lineages, concurring with our results describing the high differentiation potential of the cells from the ovary . Canine adipose‐derived stem cells have already been shown to differentiate into germ cells, so our next step was to verify if the cells derived in the present paper also possessed the capability to differentiate into putative germ cells by using the culture conditions for female germ cells derived from the ovary of neonatal mice . After 2 weeks in this media, the cells changed their morphology from fibroblast‐like to colonies of rounded cells that were positive for OCT4 and DDX4 by immunocytochemistry.…”

Section: Discussionsupporting

confidence: 89%

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

et al. 2017

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Section: Discussionsupporting

confidence: 89%

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

et al. 2017

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“…The isolated cADMSCs are positive for CD73, CD105 (Fig. S2), CD44, CD90 and CD166, whereas negative for CD34 and CD45; these cells could also differentiate into adipocytes, osteoblasts and chondrocytes under induction conditions16.…”

Section: Methodsmentioning

confidence: 98%

CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

Fang

Wei

et al. 2017

Sci Rep

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Previous studies have shown that CD61 (integrin-β3) promotes the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into germ-like cells. However, the mechanism remains unclear. In this study, we showed that overexpression of CD61 in canine adipose-derived mesenchymal stem cells (cADMSCs) promotes their differentiation into primordial germ cell (PGC)-like cells. Quantitative real-time PCR, immunocytochemistry and western blot detected higher levels of PGC-specific markers in CD61-overexpressed cADMSCs compared with those in control cells. Moreover, phosphorylation of Smad2, a downstream mediator of transforming growth factor beta (TGF-β), was increased in CD61-overexpressed cADMSCs than that in control cells. However, the expression of PGC-specific markers was downregulated in cADMSCs treated with a TGF-β inhibitor. These results suggested that CD61 could induce cADMSCs to differentiate into PGC-like cells by relying on the activation of TGF-β pathway. ADMSCs possess a considerable potential in treating the infertility of rare animal species.

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“…However, a two‐step culture method using 25 ng/ml of BMP4 for 5 days followed by 1 μM of RA for 12 days induced DAZL and STRA8 expression in mice MSC (Afsartala et al., ). Canine adipose tissue (AT) MSC, treated for 7 days with 12.5 ng/ml of BMP4, increased expression of PGC markers PRDM1, PRDM14 and male GC markers DMRT1 and PLZF but showed no effect on VASA expression (Wei et al., ). Overall, these results suggest that BMP4 regulation of GC gene expression may vary, according to concentration and time in culture medium.…”

Section: Discussionmentioning

confidence: 99%

In vitro differentiation of bovine bone marrow‐derived mesenchymal stem cells into male germ cells by exposure to exogenous bioactive factors

Cortez

Bahamonde

Reyes

et al. 2018

Reprod Domestic Animals

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Mesenchymal stem cells (MSC) are multipotent progenitor cells defined by their ability to self-renew and give rise to differentiated progeny. Previous studies have reported that MSC may be induced in vitro to develop into different types of specialized cells including male gametes. In vitro gamete derivation technology has potential applications as an alternative method for dissemination of elite animal genetics, production of transgenic animals and conservation of endangered species. This study aimed at investigating the in vitro effect of BMP4, TGFβ1 and RA on the potential for germ cell (GC) differentiation of bovine foetal MSC (bfMSC) derived from bone marrow (BM). The effect of BMP4, TGFβ1 and RA was analysed on the expression of pluripotent, GC and male GC markers on bfMSC during a 21-day culture period. bfMSC cultured under in vitro conditions expressed OCT4, NANOG and DAZL, but lacked expression of mRNA of VASA, STELLA, FRAGILIS, STRA8 and PIWIL2. Treatment with exogenous BMP4 and TGFβ1 induced a transient increase (p < .05) in DAZL and NANOG mRNA levels, respectively. However, exposure to RA was more effective in increasing (p < .05) expression of DAZL and regulating expression of OCT4 and mRNA levels of NANOG. These data suggest that bfMSC may possess potential for early GC differentiation, where OCT4, NANOG and specially DAZL may play significant roles in controlling progression along the GC lineage.

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Primordial germ cell–like cells derived from canine adipose mesenchymal stem cells

Abstract: This study provides an efficient approach to study germ cell development using CAMSCs.

Cited by 45 publications

References 40 publications

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

In vitro differentiation of bovine bone marrow‐derived mesenchymal stem cells into male germ cells by exposure to exogenous bioactive factors

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