Pro-apoptotic effect of Δ2-TGZ in “claudin-1-low” triple-negative breast cancer cells: involvement of claudin-1

Geoffroy, Marine; Kleinclauss, Alexandra; Grandemange, Stéphanie; Hupont, Sébastien; Boisbrun, Michel; Flament, Stéphane; Grillier-Vuissoz, Isabelle; Kuntz, Sandra

doi:10.1007/s10549-017-4378-2

Cited by 7 publications

(17 citation statements)

References 33 publications

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“…Thus, the re-expression of claudin 1 in MDA-MB-361 breast cancer cells induces apoptosis [20]. Our team demonstrated that claudin 1 overexpression leads to apoptosis in MDA-MB-231 and Hs578T "claudin 1 low" TNBC cells after 48 h [21]. Claudin 1 is also involved in cell motility and migration [9].…”

Section: Introductionmentioning

confidence: 82%

“…Hs578T cells were grown in DMEM medium containing phenol red (Life technologies) supplemented with 10% FCS, 2 mM l-glutamine and 10 µg/mL bovine insulin. MCF-10A cells were maintained in DMEM/F12 (GIBCO) supplemented with 5% horse serum, 1% l-glutamine 1% penicillin/streptomycin and a mix of EGF (epidermal growth factor), cholera toxin, hydrocortisone and insulin [21].…”

Section: Breast Cancer-derived Cells Culturementioning

confidence: 99%

“…Western blot experiments were performed as described previously [21]. The antibodies against occludin (13409-1-AP, Proteintech) and ZO-1 (GTX108587S, Genetex) were diluted at 1:1000.…”

Section: Western Blotmentioning

confidence: 99%

“…Stable MDA-MB-231 transfectants (8 × 10 4 cells/well) were seeded in 24-well plates on glass coverslips. Stable transfectants were grown during 72 h. Then, immunofluorescence experiments were performed as described previously [21]. Polymeric F-actin filaments were labelled with Phalloïdin conjugated to Alexa Fluor 488 (A12379, Thermofisher Scientific) diluted to 1:30.…”

Section: Immunofluorescencementioning

confidence: 99%

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Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line

et al. 2020

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Triple-negative "claudin 1 low" subtype represents around 15% of breast cancer and displays poor prognosis. The loss of claudin 1 is correlated with increased invasiveness and higher recurrence of the disease. Claudin 1 constitutes the backbone of the tight junction and is involved in cell-cell adhesion and migration processes. However, studies showed a controversial role of claudin 1 in cell migration. In this study, we aimed to clarify the effect of claudin 1 on migration of mesenchymal triple-negative breast cancer cells (TNBC). We reported that transient over expression of claudin 1 in MDA-MB-231 and Hs578T "claudin 1 low" TNBC cells inhibited cell migration using wound healing and transwell migration assays. In order to investigate more specifically the involvement of claudin 1, we generated stable MDA-MB-231 clones overexpressing claudin 1. Interestingly, the level of claudin 1 was correlated to the inhibition of cell migration and to the increase of cellcell aggregation associated with enhanced formation of β-catenin adherens junction and occludin tight junction. Finally, we reported for the first time the key role of claudin 1 in the inhibition of cell migration process associated with the disappearance of stress fibers. These data suggest that re-expression of claudin 1 could be a promising strategy for regulating the migration of TNBC which no longer express claudin 1. Keywords Triple negative breast cancer • Claudin 1 • Migration • Stress fiber • Cell junctions Abbreviations CLDN1 Claudin 1 TJ Tight junction EMT Epithelial-mesenchymal transition MSL Mesenchymal-stem-like DOCK Dedicator of cytokinesis AJ Adherent junction ZO-1 Zonula occludens-1 TNBC Triple negative breast cancer FCS Fetal calf serum PDZ-domain-binding-motif PSD95, DlgA, ZO-1 domain binding motif CSRP2 Cysteine-rich-protein 2 Electronic supplementary material The online version of this article (

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Section: Introductionmentioning

confidence: 82%

Section: Breast Cancer-derived Cells Culturementioning

confidence: 99%

“…Western blot experiments were performed as described previously [21]. The antibodies against occludin (13409-1-AP, Proteintech) and ZO-1 (GTX108587S, Genetex) were diluted at 1:1000.…”

Section: Western Blotmentioning

confidence: 99%

Section: Immunofluorescencementioning

confidence: 99%

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Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line

et al. 2020

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“…Differential interference contrast microscopy images were then obtained randomly ( Peng et al, 2018 ). The ratio of Hoechst positive cells and Tunel positive cells were respectively calculated and at least five images of each sample in different visions were observed ( Geoffroy et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Achyranthes bidentata Polypeptide Protects Schwann Cells From Apoptosis in Hydrogen Peroxide-Induced Oxidative Stress

Zhu

Peng

et al. 2018

Front. Neurosci.

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ABPPk, the active ingredient separated from Achyranthes bidentata polypeptides, is a traditional Chinese medicine with multiple pharmaceutical properties. In this study, we investigated the molecular mechanisms of ABPPk in protecting Schwann cells (SCs) from H2O2-induced cell apoptosis. The viability of SCs pretreated with ABPPk was elevated significantly by MTT assay estimation. Meanwhile, the apoptosis of SCs was reduced which was showed in flow cytometry and transferase-mediated dUTP nick end labeling analysis. Furthermore, the addition of ABPPk also increased the activities of SOD and GSH accompanied with a decrease in MDA and LDH activities. According to Western blot analysis, the upregulation of Bcl-2, also downregulation of Bax and cleaved caspase-3 were demonstrated in SCs which was ABPPk pretreated. Further research showed that PI3K/AKT and ERK1/2 pathways in SCs have been activated after pretreatment of ABPPk. Collectively, results in our study suggested that ABPPk protected SCs from H2O2-induced oxidative damage by reducing the expression of apoptotic molecules and enhancing the activities of antioxidant enzymes, which inhibited the apoptosis of SCs modulated by PI3K/AKT and ERK1/2 signaling pathways. In our perspectives, ABPPk as an active factor with its antioxidative activities has potential and promising therapeutic effects in the prevention of neurologic disorders.

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Claudin 1: An Emerging Target for Triple-Negative Breast Cancer

Grillier-Vuissoz,

Geoffroy,

Kuntz

2024

Interdisciplinary Cancer Research

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Pro-apoptotic effect of Δ2-TGZ in “claudin-1-low” triple-negative breast cancer cells: involvement of claudin-1

Cited by 7 publications

References 33 publications

Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line

Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line

Achyranthes bidentata Polypeptide Protects Schwann Cells From Apoptosis in Hydrogen Peroxide-Induced Oxidative Stress

Claudin 1: An Emerging Target for Triple-Negative Breast Cancer

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