Probing the interactions of dansyl appended sequence-defined oligomers with serum albumins: Effect of functionality, hydrophobicity, and architecture

Jose, Anna; Porel, Mintu

doi:10.1016/j.jphotochem.2023.114640

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…One of the most essential characteristics for the druggable nature of a molecule is its ability to bind with a protein. Nanoparticle‐based drugs are currently being investigated extensively in biomedical domains such as targeted drug delivery, imaging and diagnostic of disease, disease control and monitoring, and high drug‐loading formulation, including solubility, stability, and high bioavailability 29 . Herein, a protein binding study was performed using the most prevalent serum protein in the blood to better understand the druggable nature of the synthesized fluorescent AgNPs.…”

Section: Resultsmentioning

confidence: 99%

“…Nanoparticle-based drugs are currently being investigated extensively in biomedical domains such as targeted drug delivery, imaging and diagnostic of disease, disease control and monitoring, and high drug-loading formulation, including solubility, stability, and high bioavailability. 29 Herein, a protein binding study was performed using the most prevalent serum protein in the blood to better understand the druggable nature of the synthesized fluorescent AgNPs. The fluorescence spectroscopy was used to conduct the protein binding experiment, taking advantage of the inherent fluorescence characteristics of tryptophan and tyrosine residues.…”

Section: Fe-sem and Temmentioning

confidence: 99%

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Dansyl‐tagged xanthate ester as a capping agent to synthesize fluorescent silver nanoparticles with binding affinity toward serum albumin

Barik,

Pidikaka,

Porel

2024

Photochem & Photobiology

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Developing multifunctional nanomaterials with distinct photochemical properties, such as high quantum yield, improved photostability, and good biocompatibility is critical for a wide range of biomedical applications. Motivated by this, we designed and synthesized a dansyl‐tagged xanthate‐based capping agent (DX) for the synthesis of fluorescent silver nanoparticles (AgNPs). The capping agent DX was characterized by 1H and 13C‐NMR, LC–MS, and FT‐IR. The synthesized DX‐capped fluorescent AgNPs were thoroughly characterized by UV–visible spectroscopy, fluorescence spectroscopy, field emission scanning electron microscope (FE‐SEM), transmission electron microscope (TEM), dynamic light scattering (DLS), and zeta potential. The fluorescent AgNPs showed distinct surface plasmon resonance absorption at λmax = 414 nm, fluorescence at λmax = 498 nm, quantum yield = 0.24, zeta potential = +18.6 mV, average size = 18.2 nm. Furthermore, the biological activity of the fluorescent AgNPs was validated by its interaction with the most abundant protein in the blood, that is, BSA (Bovine serum albumin) and HSA (Human serum albumin) with binding constant of 2.34 × 104 M−1 and 2.14 × 104 M−1 respectively. Interestingly, fluorescence resonance energy transfer (FRET) was observed between the fluorescent AgNPs and BSA/HSA with a FRET efficiency of 77.23% and 56.36%, respectively, indicating strong interaction between fluorescent AgNPs and BSA/HSA.

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Section: Resultsmentioning

confidence: 99%

Section: Fe-sem and Temmentioning

confidence: 99%

Dansyl‐tagged xanthate ester as a capping agent to synthesize fluorescent silver nanoparticles with binding affinity toward serum albumin

Barik,

Pidikaka,

Porel

2024

Photochem & Photobiology

Self Cite

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show abstract

“…To find the druggable nature of a compound, and to find the safety and efficacy of a drug, drug-protein interaction studies are inevitable [43][44][45][46][47]. When we administer a drug molecule into our body, it interacts with various proteins of our body, including structural proteins, enzymes, receptors, and transporters.…”

Section: Introductionmentioning

confidence: 99%

A Novel Class of Functionally Tuneable Star-Shaped Molecules for Interaction with Multiple Proteins

et al. 2023

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Molecules with tuneable properties are well known for their applications in the material and bio-medical fields; nevertheless, the structural and functional tunability makes them more significant in diverse applications. Herein, we designed and synthesized a novel class of star-shaped molecules via incorporating two important functional groups, i.e., triazole and dithiocarbamate (DTC). The rationale behind selecting these two key functional groups is their diverse applications, e.g., DTC having applications for therapeutics, pesticides, and vulcanizing agents, and triazole having applications for anti-cancer, fungicides, anti-microbials, inhibitors, etc. The structure of the molecules was strategically designed in such a way that their overall structures are the same (central tertiary-amine and peripheral hydroxy groups), except the key functional group (DTC and triazole) in the respective molecules was different. Following synthesis and characterization, the influence of DTC and triazole groups on their bioactivity was compared via interacting with the most abundant proteins present in the blood, including serum albumin, trypsin, haemoglobin, and ribonuclease. From both the experimental and molecular docking studies, it was confirmed that the triazole molecule has a higher binding affinity towards these proteins as compared to the DTC molecule. In summary, two star-shaped DTC- and triazole-based molecules were synthesized and their bioactivity was compared via binding with blood plasma proteins.

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Enhancing water sensing via aggregation-induced emission (AIE) and solvatofluorochromic studies using two new dansyl derivatives containing a disulfide bound: Pollutant metal ions detection and preparation of water-soluble fluorescent polymeric particles

et al. 2023

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Probing the interactions of dansyl appended sequence-defined oligomers with serum albumins: Effect of functionality, hydrophobicity, and architecture

Cited by 4 publications

References 34 publications

Dansyl‐tagged xanthate ester as a capping agent to synthesize fluorescent silver nanoparticles with binding affinity toward serum albumin

Dansyl‐tagged xanthate ester as a capping agent to synthesize fluorescent silver nanoparticles with binding affinity toward serum albumin

A Novel Class of Functionally Tuneable Star-Shaped Molecules for Interaction with Multiple Proteins

Enhancing water sensing via aggregation-induced emission (AIE) and solvatofluorochromic studies using two new dansyl derivatives containing a disulfide bound: Pollutant metal ions detection and preparation of water-soluble fluorescent polymeric particles

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