Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals

Hepburn, Natalie J.; Garaiová, Iveta; Williams, Elizabeth A.; Michael, D R; Plummer, Susan

doi:10.3920/bm2013.0012

Cited by 15 publications

(14 citation statements)

References 21 publications

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“…Compositional similarities between the blood and lower airway microbiome bring into question the possibility of GI tract bacterial translocation from the gut through the bloodstream as a route of entry; however, the bacterial communities in the GI tract were significantly different from those in blood and BAL. Perhaps as has been suggested in other studies, a systemic effect of oral probiotics (e.g., alteration of cytokine concentrations) may have modulated the respiratory microbiota (Vitali et al, 2012; Dai et al, 2013; Hepburn et al, 2013; Mastromarino et al, 2015). …”

Section: Discussionmentioning

confidence: 68%

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

et al. 2017

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Probiotics have been advocated as a novel therapeutic approach to respiratory disease, but knowledge of how oral administration of probiotics influences the respiratory microbiota is needed. Using 16S rRNA amplicon sequencing of bacterial DNA our objective was to determine whether oral probiotics changed the composition of the upper and lower airway, rectal, and blood microbiota. We hypothesized that oral probiotics would modulate the respiratory microbiota in healthy cats, demonstrated by the detection and/or increased relative abundance of the probiotic bacterial species and altered composition of the microbial population in the respiratory tract. Six healthy young research cats had oropharyngeal (OP), bronchoalveolar lavage fluid (BALF), rectal, and blood samples collected at baseline and 4 weeks after receiving oral probiotics. 16S rRNA gene amplicon libraries were sequenced, and coverage, richness, and relative abundance of representative operational taxonomic units (OTUs) were determined. Hierarchical and principal component analyses (PCA) demonstrated relatedness of samples. Mean microbial richness significantly increased only in the upper and lower airways. The number of probiotic OTUs (out of 5 total) that significantly increased in relative abundance vs. baseline was 5 in OP, 3 in BAL and 2 in feces. Using hierarchical clustering, BALF and blood samples grouped together after probiotic administration, and PERMANOVA supported that these two sites underwent significant changes in microbial composition. PERMANOVA revealed that OP and rectal samples had microbial population compositions that did not significantly change. These findings were visualized via PCA, which revealed distinct microbiomes in each site; samples clustered more tightly at baseline and had more variation after probiotic administration. This is the first study describing the effect of oral probiotics on the respiratory microbiota via detection of probiotic species in the airways. Finding bacterial species present in the oral probiotics in the upper and lower airways provides pilot data suggesting that oral probiotics could serve as a tool to target dysbiosis occurring in inflammatory airway diseases such as feline asthma, a disease in which cats serve as an important comparative and translational model for humans.

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Section: Discussionmentioning

confidence: 68%

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

et al. 2017

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“…lactis and B . bifidum combined with FOS, showed a significant decrease in proinfammatory cytokines IL-6 and IL-1β when peripheral blood mononuclear cells were isolated and cultured ex vivo in presence of lipopolysaccharide [29]. In a randomized, double-blind, placebo-controlled trial three different probiotic strains were supplemented for 30 days.…”

Section: Discussionmentioning

confidence: 99%

Effects of Supplementation of the Synbiotic Ecologic® 825/FOS P6 on Intestinal Barrier Function in Healthy Humans: A Randomized Controlled Trial

et al. 2016

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Background and AimsProbiotics, prebiotics and synbiotics have been suggested as dietary strategies to improve intestinal barrier function. This study aimed to assess the effect of two weeks synbiotic supplementation on intestinal permeability under basal and stressed conditions. Secondary aims were the assessment of two weeks synbiotic supplementation on systemic immune function and gastrointestinal symptoms including defecation pattern.DesignTwenty healthy adults completed a double-blind, controlled, randomized, parallel design study.InterventionGroups either received synbiotic (1.5 × 1010 CFU Ecologic® 825 + 10 g fructo-oligosaccharides (FOS P6) per day) or control supplements for two weeks.OutcomesIntestinal segment specific permeability was assessed non-invasively by oral administration of multiple sugar probes and, subsequently, assessing the excretion of these probes in urine. This test was conducted at baseline and at the end of intervention, in the absence and in the presence of an indomethacin challenge. Indomethacin was applied to induce a compromised gut state. Plasma zonulin, cytokines and chemokines were measured at baseline and at the end of intervention. Gastrointestinal symptoms and stool frequency were recorded at baseline and daily during intervention.ResultsSignificantly more male subjects were in the synbiotic group compared to the control group (P = 0.025). Indomethacin significantly increased urinary lactulose/rhamnose ratio versus without indomethacin, both in the control group (P = 0.005) and in the synbiotic group (P = 0.017). Urinary sugar recoveries and ratios, plasma levels of zonulin, cytokines and chemokines, and gastrointestinal symptom scores were not significantly different after control or synbiotic intervention. Stool frequency within the synbiotic group was significantly increased during synbiotic intervention compared to baseline (P = 0.039) and higher compared to control intervention (P = 0.045).ConclusionTwo weeks Ecologic® 825/FOS P6 supplementation increased stool frequency, but did not affect intestinal permeability neither under basal nor under indomethacin-induced stressed conditions, immune function or gastrointestinal symptoms in healthy adults.

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“…and Lactobacillus spp. Supplementation with Lab4b has been shown to reduce the incidence of allergy in infants (Allen et al, 2010(Allen et al, , 2014 whilst Lab4 impacted on both inflammation (Davies et al, 2018;Garaiova et al, 2015;Hepburn et al, 2013) and metabolism (Michael et al, 2016(Michael et al, , 2017. Some probiotic bacteria are known producers of metabolites with neuromodulatory capability (Sarkar et al, 2016) and Lab4 has been shown to improve memory and alter brain metabolite profiles in aging rats (O'Hagan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro neuroprotective activities of two distinct probiotic consortia

Michael

Davies

Loxley

et al. 2019

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Neurodegeneration has been linked to changes in the gut microbiota and this study compares the neuroprotective capability of two bacterial consortia, known as Lab4 and Lab4b, using the established SH-SY5Y neuronal cell model. Firstly, varying total antioxidant capacities (TAC) were identified in the intact cells from each consortia and their secreted metabolites, referred to as conditioned media (CM). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Crystal Violet (CV) assays of cell viability revealed that Lab4 CM and Lab4b CM could induce similar levels of proliferation in SH-SY5Y cells and, despite divergent TAC, possessed a comparable ability to protect undifferentiated and retinoic acid-differentiated cells from the cytotoxic actions of rotenone and undifferentiated cells from the cytotoxic actions of 1-methyl-4-phenylpyridinium iodide (MPP+). Lab4 CM and Lab4b CM also had the ability to attenuate rotenone-induced apoptosis and necrosis with Lab4b inducing the greater effect. Both consortia showed an analogous ability to attenuate intracellular reactive oxygen species accumulation in SH-SY5Y cells although the differential upregulation of genes encoding glutathione reductase and superoxide dismutase by Lab4 CM and Lab4b CM, respectively, implicates the involvement of consortia-specific antioxidative mechanisms of action. This study implicates Lab4 and Lab4b as potential neuroprotective agents and justifies their inclusion in further in vivo studies.

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Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals

Cited by 15 publications

References 21 publications

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

Oral Probiotics Alter Healthy Feline Respiratory Microbiota

Effects of Supplementation of the Synbiotic Ecologic® 825/FOS P6 on Intestinal Barrier Function in Healthy Humans: A Randomized Controlled Trial

In vitro neuroprotective activities of two distinct probiotic consortia

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