Process development of continuous hydrogen production by Enterobacter aerogenes in a packed column reactor

Abstract

Hydrogen bioproduction from agro-industrial residues by Enterobacter aerogenes in a continuous packed column has been investigated and a complete reactor characterization is presented. Experimental runs carried out at different residence time, liable of interest for industrial application, showed hydrogen yields ranging from 1.36 to 3.02 mmol H 2 mmol À1 glucose or, in other words, from 37.5% to 75% of the theoretical hydrogen yield. A simple kinetic model of cell growth, validated by experimental results and allowing the prediction of biomass concentration pro®le along the reactor and the optimization of super®cial velocity, is suggested. By applying the developed approach to the selected operative conditions, the identi®cation of the optimum super®cial velocity v 0,opt of about 2.2 cm h )1 corresponding to the maximum hydrogen evolution rate H 2gYmax , was performed.

Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17

“…and facultative anaerobes (such as Enterobacter sp.) are often used for hydrogen generation [1][2][3]. Microflora are also used to generate hydrogen [4][5][6][7][8].…”

Biological hydrogen production in suspended and attached growth anaerobic reactor systems

“…Most studies on biological fermentative hydrogen production from sugars using mixed cultures have been conducted in conventional CSTR under mesophilic conditions [14][15][16][17]. Recently, hydrogen production in anaerobic systems with selfgranulated hydrogenogenic biomass [18][19][20] and with attached microbial growth on support material [21][22][23][24][25] received attention as well. Palazzi et al [21] investigated the hydrogen production from starch hydrolysate using a pure culture of Enterobacter aerogenes grown on a mixture of spongy and glass beads in a packed column reactor.…”

“…Recently, hydrogen production in anaerobic systems with selfgranulated hydrogenogenic biomass [18][19][20] and with attached microbial growth on support material [21][22][23][24][25] received attention as well. Palazzi et al [21] investigated the hydrogen production from starch hydrolysate using a pure culture of Enterobacter aerogenes grown on a mixture of spongy and glass beads in a packed column reactor. Kumar and Das [22] used immobilised microbial cells of Enterobacter cloacae on lignocellulosic material for continuous hydrogen production from soluble starch.…”

Hydrogen production of a salt tolerant strain Bacillus sp. B2 from marine intertidal sludge

“…The facultative anaerobes have an advantage over the obligate ones on the cultivation because the latter need strict anaerobic conditions (Yokoi et al 1998). The capability of hydrogen production has been examined in some H. Liu Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine Science and Engineering, University of Science and Technology, Tianjin 300457, China e-mail: hongyanliu1214@163.com facultative anaerobes, such as Enterobacter Cloacae (Kumar and Das 2001) and Enterobacter aerogenes HU-101, AY-2, and HO-39 (Palazzi et al 2000;Yokoi et al 1995).…”