Process optimization for cultivation and oil accumulation in an oleaginous yeast Rhodosporidium toruloides A29

Saran, Saurabh; Mathur, Arushi; Dalal, Jyotsana; Saxena, Rajendra Kumar

doi:10.1016/j.fuel.2016.09.051

Cited by 55 publications

(20 citation statements)

References 34 publications

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“…Response surface methodology is a statistical method that is used to optimize an operational condition with multiple variables and study the relationship between each independent variable and its response [ 42 , 43 ]. BBD was used for RSM in the experimental design, which is well suited for determining the optimal conditions for multifactorial systems quickly and effectively [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans

Wang

Fan

et al. 2018

Biotechnol Biofuels

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BackgroundThe release of refined soybean oil wastewater (RSOW) with a high chemical oxygen demand (COD) and oil content burdens the environment. The conversion of RSOW into lipids by oleaginous yeasts may be a good way to turn this waste into usable products.ResultsThe oleaginous yeast Trichosporon fermentans was used for treating the RSOW without sterilization, dilution, or nutrient supplementation. It was found that the COD and oil content of the RSOW were removed effectively; microbial oil was abundantly produced in 48 h; and the phospholipids in the RSOW tended to contribute to a higher biomass and microbial lipid content. With Plackett–Burman design and response surface design experiments, the optimal wastewater treatment conditions were determined: temperature 28.3 °C, amount of inoculum 5.9% (v/v), and initial pH 6.1. The optimized conditions were used in a 5-L bioreactor to treat the RSOW. The maximum COD degradation of 94.7% was obtained within 40 h, and the removal of the oil content was 89.9%. The biomass was 7.9 g/L, the lipid concentration was 3.4 g/L, and the lipid content was 43% (w/w). The microbial oil obtained, with a main component of unsaturated fatty acids, was similar to vegetable oils and was suggested as a potential raw material for biodiesel production.ConclusionTrichosporon fermentans can be effectively used for RSOW treatment, and lipid production and can complete pretreatment and biochemical treatment simultaneously, allowing the utilization of RSOW, which both solves an environmental problem and positively impacts the use of resources. These results provide valuable information for developing and designing more efficient waste-into-lipid bioprocesses.

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Section: Methodsmentioning

confidence: 99%

Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans

Wang

Fan

et al. 2018

Biotechnol Biofuels

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“…In recent decades, the production of lipids using lignocellulosic hydrolysates and oil yeasts has become an attractive alternative way to obtain biofuels, chemical commodities, or precursors for the production of different compounds. In general, the accumulation of lipids in oleaginous yeast is carried out through two routes ( de novo and ex novo ), under limited nitrogen conditions in the medium . The production of lipids by native strains of the genus Rhodosporidium using hydrolysates obtained from lignocellulosic biomass as a substrate is very limited because this type of hydrolysate contains toxic compounds that greatly affect the development and viability of the microorganism …”

Section: Challenges In Lipid Production In Rhodosporidium Genrementioning

confidence: 99%

“…To reduce these feedstock costs, different carbohydrates have been obtained from lignocellulosic biomass from forestry residues . Various studies in the literature have reported the use of different culture media, such as glucose, xylose, crude glycerol, acetic acid, sugarcane juice, and hydrolysates from different kinds of lignocellulosic biomass as well as different cultivation systems (flask, batch, fed, fed‐batch, and pilot) .…”

Section: Introductionmentioning

confidence: 99%

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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The use of lignocellulosic biomass biofuels is an attractive alternative because they do not put food safety at risk, they are a renewable source, and their use is limited. The use of microorganisms has now become more widespread to take advantage of the carbohydrates present in this raw material (cellulose and hemicellulose) and the products of its hydrolysis (glucose, xylose, arabinose, galactose, and mannose). The fatty acids obtained from oleaginous microorganisms are potential sources for the production of drop‐in biofuels. Rhodosporidium sp. is an oleaginous yeast that accumulates up to 70% of its biomass in the form of lipids and is highly adaptable to different types of substrates. This review discusses the different factors and challenges (genetic modification of strains, pretreatments, and inhibitor effects) in obtaining lipid from lignocellulosic biomass using Rhodosporidium sp. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Previously, Nouri et al [41] successfully optimized the culture condition of Sarocladium kiliense ADH17 using the RSM method to improve the lipid to dry weight ratio, biomass, and lipid production, which were improved by 32.5%, 45%, and 30%, respectively [41]. Optimization of medium components of Rhodosporidium toruloides A29 using the RSM method and scale-up to 30 L could achieve a 22-fold increase in lipid yield [42]. Under the optimum conditions obtained by the RSM method (glycerol 89 g/L, ammonium hydroxide 0.54 g/L, incubation time 66 h), Y. lipolytica could produce the biomass and lipid content of 25.0 ± 1.5 g/L and 52.7 ± 1.2% (w/w of dry biomass), which was increased by 64% and 20% compared to un-optimized conditions [43].…”

Section: Contentmentioning

confidence: 99%

Multi-objective optimization for biomass and lipid production by oleaginous bacteria using vegetable waste as feedstock

Patnaik

Saravanabhupathy

Singh

et al. 2021

Environmental Engineering Research

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In this study, pretreated organic wastes such as waste paper cups, cardboard waste, and vegetable waste were screened for the growth and lipid production of oleaginous bacteria DS-7 (isolated from the dairy effluent scum). The pretreated vegetable waste was found to be the best feedstock for biomass and lipid production by the DS-7. Further, process parameters such as inoculation time, substrate concentration (w/v) (amount of pretreated vegetable waste), pH, and inoculum size were optimized using a multi-objective optimization technique to enhance the biomass and lipid productions. The optimization study successfully enhanced the biomass concentration (g/L) and lipid content (%) by 47.9% and 15.84%, respectively in comparison with the unoptimized state. The biomass and lipid productivities were 42% (1.449 g/L/d) and 51% (1.267 g/L/d) greater than unoptimized conditions. The characteristics of the biodiesel obtained from the valorization of vegetable waste were comparable to the standard. Thus, the vegetable waste can be utilized as a potential feedstock for microbial biodiesel production.

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Process optimization for cultivation and oil accumulation in an oleaginous yeast Rhodosporidium toruloides A29

Cited by 55 publications

References 34 publications

Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans

Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans

Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Multi-objective optimization for biomass and lipid production by oleaginous bacteria using vegetable waste as feedstock

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