Product qualification: a barrier to point-of-care microfluidic-based diagnostics?

Tantra, Ratna; Heeren, Henne van

doi:10.1039/c3lc50246e

Cited by 25 publications

(12 citation statements)

References 15 publications

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“…1 illustrates a sealed microfluidic device having fluidic and electrical connections. Typically, after the fabrication of microfluidic structures and electrodes, various back-end processes are needed to realize a fully-functional device [15,16]. Taking the example in Fig.…”

Section: Introductionmentioning

confidence: 99%

Lab-on-a-chip devices: How to close and plug the lab?

Temiz

Lovchik

Kaigala

et al. 2015

Microelectronic Engineering

416

276

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a b s t r a c tLab-on-a-chip (LOC) devices are broadly used for research in the life sciences and diagnostics and represent a very fast moving field. LOC devices are designed, prototyped and assembled using numerous strategies and materials but some fundamental trends are that these devices typically need to be (1) sealed, (2) supplied with liquids, reagents and samples, and (3) often interconnected with electrical or microelectronic components. In general, closing and connecting to the outside world these miniature labs remain a challenge irrespectively of the type of application pursued. Here, we review methods for sealing and connecting LOC devices using standard approaches as well as recent state-of-the-art methods. This review provides easy-to-understand examples and targets the microtechnology/engineering community as well as researchers in the life sciences.

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Section: Introductionmentioning

confidence: 99%

Lab-on-a-chip devices: How to close and plug the lab?

Temiz

Lovchik

Kaigala

et al. 2015

Microelectronic Engineering

416

276

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“…Such drawback inhibits the creation of profitable businesses. 17 We address herein a POC platform for performing the Bradford assay using smartphone-based optical detection. The smartphones have been widely used to the development of POC analytical tools for different colorimetric quantitative analyses, including immunoassays, biochemical and healthcare experiments.…”

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confidence: 99%

Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

Camargo¹,

Vicentini²,

Gobbi³

et al. 2016

J. Braz. Chem. Soc.

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We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%. Keywords: rapid test, albumin, human plasma, colorimetry, negatoscope IntroductionBradford assay is one of the most usual methods applied for determining protein in solution. [1][2][3][4] This method was proposed by Marion M. Bradford in 1976 5 and relies on the interaction of Comassie Brilliant Blue G-250 dye with the protein. After a short-time incubation, the anionic product of such reaction is optically monitored at 595 nm. 6 The advantages of the Bradford assay are that its routine is user-friendly, fast, reproducible, sensitive and selective to the target molecule. 7,8 Conversely, some pharmaceutical excipients interfere on the response of this approach even at low contents. In this case, the precipitation of the protein with sucrose showed to be an efficient alternative.9 Other bottleneck concerns the poor portability and relatively high cost (ca. US$ 10,000.00) of the spectrophotometer applied in the Bradford assays. These features undermine the compatibility of the method toward point-of-care (POC) analyses. 10POC platforms present low cost and high portability, robustness and simplicity. Accordingly, such technologies bypass the necessity for specialized personal and allow in situ measurements even at resource-limited environments, presenting key social and economic implications. Such methods have been recently used at diverse applications. [11][12][13][14][15][16] The POC technology is the major segment of the global in vitro diagnostics market. 11Nonetheless, despite the advantages of these methods, only a few commercial executions had been observed because of the production cost of such analytical platforms. Such drawback inhibits the creation of profitable businesses. 17We address herein a POC platform for performing the Bradford assay using smartphone-based optical detection. The smartphones have been widely used to the development of POC analytical tools for different colorimetric quantitative analyses, including immunoassays, biochemical and healthcare experiments. [18][19][20][21][22] These applications are possible because the smartphones present the two functional components necessary for accomplishing optical detection: source (light-emitting diode, LED, white light from flash) and radiation detector (digital camera). 23 This camera exhibits resolution and sensitivity greater than those of conventional phone camera...

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“…Several reviews have analyzed the challenges involved in LOC commercialization process, explaining some of the reasons for the delayed spread of devices into mainstream healthcare and highlighting aspects that are necessary for successful commercialization [9][10][11]. First, it is important to address LOC designs as a total analysis system rather than focusing on discrete modules, while still maintaining a clear and specific final analytical goal.…”

Section: Introductionmentioning

confidence: 99%