Production and Characterization of IgY against Canine IgG: Prospect of a New Tool for the Immunodiagnostic of Canine Diseases

Abstract

This study describes the production of a new avian polyclonal antibody (IgY)

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

“…IgY can be purified through the adsorption of the immunizing antigen to the solid phase of the affinity column, which generates monospecific antibodies that can be eluted under acid [53] or alkaline conditions [54]. The purification of IgY can also be performed through thiophilic adsorption chromatography [55]. In contrast to other affinity purification methods, it can be performed at mild conditions [56].…”

Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements

“…Electrophoresis carried out in reducing conditions should provide two bands that can be correlated with IgY, one corresponding to the heavy chain (HC) and the other to the light chain (LC). Molecular weight of HC and LC of IgY according to literature are 68 and 27 kDa respectively [3]. Figure 6a, lanes 1-4, shows many protein bands, both in the samples containing fresh egg yolk and freeze-dried egg yolk.…”

“…Concerning the structure, IgY is more similar to mammalian IgE, since the Hinge region is not present in its molecular structure [2]. IgY is composed of two heavy chains (M w = 68 kDa) and two light chains (M w = 27 kDa), resulting in a molecular weight of around 190 kDa [3]. The concentration of this antibody in hen blood serum is 5-15 mg/mL, in egg yolk is 10-25 mg/mL; for this high concentration and for its complete absence in egg white, IgY is well representative of egg yolk [4].…”

“…Among all of them, the most promising use of IgY is in immunotherapy, as shown by the many reviews and articles recently published covering this subject [2][3][4][5]; for example, IgY can protect humans and mammalians against animal diseases caused by virus or bacteria attacks. For these purposes, IgY can be added to many foods, both for cattle and humans (kids and adults), to improve the immunological properties of aliments.…”

Utilization of Immunoglobulin Y (IgY) as Alternatives to Laboratory Animals in Human and Veterinary Medical Practice: A Review

“…On a large scale and for industrial purposes, the existing polyclonal antibody production using mammals is less successful due to increased blood necessity in the view of producing significant antibodies for wider application (Kovacs-Nolan and Mine, 2004). In such circumstances, chicken IgY is an effective and alternative method with less animal usage and specific immunoglobulin production (Schade et al, 2005;Diraviyam et al, 2014;Santos et al, 2014;Abbas et al, 2019;Pereira et al, 2019).…”