Production and characterization of monoclonal antibodies against the "brain-specific" proteins 14-3-2 and S-100.

Haan, Eric; Boss, Barbara D.; Cowan, William

doi:10.1073/pnas.79.23.7585

Cited by 69 publications

(31 citation statements)

References 33 publications

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“…As Gfap is known to have an NFI binding site in its promoter (Cebolla and Vallejo, 2006), we asked whether the levels of other astrocytic markers were also reduced. Transcript levels of s100␤, a calcium-binding protein specifically expressed in astrocytes (Haan et al, 1982), were significantly reduced in E18 Nfia Ϫ/Ϫ versus wild-type hippocampi (supplemental Fig. 6, available at www.jneurosci.org as supplemental material).…”

Section: Delayed Vz Differentiation Culminates In Deficient Glial Matmentioning

confidence: 99%

NFIA Controls Telencephalic Progenitor Cell Differentiation through Repression of the Notch Effector Hes1

Piper¹,

Barry²,

Hawkins³

et al. 2010

Journal of Neuroscience

141

145

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The balance between self-renewal and differentiation of neural progenitor cells is an absolute requirement for the correct formation of the nervous system. Much is known about both the pathways involved in progenitor cell self-renewal, such as Notch signaling, and the expression of genes that initiate progenitor differentiation. However, whether these fundamental processes are mechanistically linked, and specifically how repression of progenitor self-renewal pathways occurs, is poorly understood. Nuclear factor I A (Nfia), a gene known to regulate spinal cord and neocortical development, has recently been implicated as acting downstream of Notch to initiate the expression of astrocyte-specific genes within the cortex. Here we demonstrate that, in addition to activating the expression of astrocyte-specific genes, Nfia also downregulates the activity of the Notch signaling pathway via repression of the key Notch effector Hes1. These data provide a significant conceptual advance in our understanding of neural progenitor differentiation, revealing that a single transcription factor can control both the activation of differentiation genes and the repression of the self-renewal genes, thereby acting as a pivotal regulator of the balance between progenitor and differentiated cell states.

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Section: Delayed Vz Differentiation Culminates In Deficient Glial Matmentioning

confidence: 99%

NFIA Controls Telencephalic Progenitor Cell Differentiation through Repression of the Notch Effector Hes1

Piper¹,

Barry²,

Hawkins³

et al. 2010

Journal of Neuroscience

141

145

View full text Add to dashboard Cite

show abstract

“…S100[3 is a 20-kD calcium-binding protein expressed in the cytoplasm of astrocytes (Matus and Mughal 1975;Haan et al 1982;Zimmer and Van Eldik 1987). It could act in glia to alter calcium-dependent processes or it could also be released and stimulate neurons directly to increase the free intracellular Ca 2+ levels (Barger and Van Eldik 1992).…”

Section: Abnormal Learning and Ltd In 5100~ Transgenic Micementioning

confidence: 99%

Overexpression of a calcium-binding protein, S100 beta, in astrocytes alters synaptic plasticity and impairs spatial learning in transgenic mice.

Gerlai

Wojtowicz²,

Marks³

et al. 1995

Learn. Mem.

129

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“…In brain, the S-IDO protein is found in both protoplasmic and fibrillary astrocytes (20). The astroglial localization and the high solubility of S-loo makes this protein a suitable marker for an astroglial cell increase in response to brain damage (7,38,40).…”

Section: Discussionmentioning

confidence: 99%

Astrogliosis in the cerebral cortex of gerbils after long-term exposure to 1,1,1-trichloroethane.

Rosengren¹,

Aurell²,

Kjellstrand³

et al. 1985

Scand J Work Environ Health

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Astrogliosis in the cerebral cortex of gerbils after long-term exposure to 1,1,1-trichloroethane. by Rosengren LE, Aurell A, Kjellstrand P, Haglid KGThe following article refers to this text: 0;0 Special issue:0 11 (1985) [447][448][449][450][451][452][453][454][455] Astrogliosis in the cerebral cortex of gerbils after long-term exposure to 1,1,1-trichloroethane by Lars E Rosengren, MD,1 Ann Aurell, 88,1 Per Kjellstrand, PhD,2 Kenneth G Haglid, MD 1 ROSENGREN LE, AURELL A, KJELLSTRAND P, HAGLID KG. Astrogliosis in the cerebral cortex of gerbils after long-term exposu re to l ,l ,l-trichloroethane . Scand J Work En viron Health 11 (1985) 447-455 . Mongolian gerbils (Meriones ungiculatus) were continuously expos ed by inhalation to l,l,l-trichloroethane at 70, 210, or 1000 ppm for three months, followed by a four-month postexposure solvent-free period. Concentration s of two astroglial proteins, S-Ioo and glial fibrillary acidic (GFA) protein, were then determined in different regions of the cerebral cortex. The main biochemical alterations induced after exposure to 210 and I 000 ppm of I, I, l-t richloroethane demonst rated a pronounced change in gerbil bra in; increased concent rations of GFA protein were found in the cerebral sensorimotor corte x at both these exposure levels, an occur rence indicating astrogliosis in this brain region . These results suggest that 1,1.l-trichloroethane should not be regarded as harmless, particularly regarding neurotoxicity, as previously claimed .

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Production and characterization of monoclonal antibodies against the "brain-specific" proteins 14-3-2 and S-100.

Abstract: We have raised mouse hybridomas that secrete monoclonal antibodies against bovine brain-specific proteins 14-

Cited by 69 publications

References 33 publications

NFIA Controls Telencephalic Progenitor Cell Differentiation through Repression of the Notch Effector Hes1

NFIA Controls Telencephalic Progenitor Cell Differentiation through Repression of the Notch Effector Hes1

Overexpression of a calcium-binding protein, S100 beta, in astrocytes alters synaptic plasticity and impairs spatial learning in transgenic mice.

Astrogliosis in the cerebral cortex of gerbils after long-term exposure to 1,1,1-trichloroethane.

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