Production and characterization of somatic hybrids between Brassica napus and Raphanus sativus

Wang, Y. P.; Sonntag, K.; Rudloff, E.; Groeneveld, I.; Gramenz, J.; Chu, C. Y. Cyrus

doi:10.1007/s11240-006-9118-y

Cited by 22 publications

(10 citation statements)

References 9 publications

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“…Due to the extensive efforts undertaken for several important plants with regards to protoplast fusion and somatic hybridization, several reviews relating to specific plants such as citrus (Grosser et al 2000), potato (Millam et al 1995;Orczyk et al 2003) and tomato (Lefrancois et al 1993) have been published. Many of the research efforts focused on the most economically important crop plants such as rice (e.g., Zhu et al 2004;Feng et al 2006), wheat (Ge et al 2006), the Brassicaceae (Wang et al 2003(Wang et al , 2005(Wang et al , 2006 and potato (Bidani et al 2007;Thieme et al 2008).…”

Section: Technology-based Breedingmentioning

confidence: 99%

Functional Genomics For Crop Improvement

Ganeshan

Sharma

Chibbar

2009

Molecular Techniques in Crop Improvement

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Plant breeding has had a tremendous influence on crop improvement. However, due to dwindling germplasm resources, identification of variability for incorporation into new cultivars is becoming more difficult. Therefore, there has been recourse to alternative approaches including mutagenesis, tissue culture and genetic transformation to aid breeding programs. Furthermore, with the vast repertoire of genome-wide data from different expression profiling techniques such as microarrays, more subtle understanding of gene expression is being obtained and is further helping plant breeders to entertain a different selection approach based on expression quantitative traits to maximize combinations of genes capable of conferring high performance. In this chapter, we review some of the aspects of plant breeding and the influence functional genomics has on breeding programs. Some of the challenges to functional genomics and breeding come from establishment of high-throughput transformation systems to assess gene function, which is limiting functional characterization of numerous genes in their respective crops. Therefore, this chapter also focuses on the need to gain better understanding of the development of gene transfer systems for crop plants to make use of the array of available gene information data.

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Section: Technology-based Breedingmentioning

confidence: 99%

Functional Genomics For Crop Improvement

Ganeshan

Sharma

Chibbar

2009

Molecular Techniques in Crop Improvement

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“…The possibility of circumventing barriers to sexual reproduction and the effective use of valuable germplasm, which are the benefits of somatic hybridization (Hansen and Earle 1997;Glimelius, 1999), have been demonstrated successfully within the Brassicaceae family, which is very amenable to protoplast fusion. Protoplast fusion has enabled the cultivated Brassica allopolyploids to be resynthesized and make easy intergeneric or even intertribal hybrids between Brassica crops and relatives in several genera or tribes (Sigareva and Earle 1997;Wang et al 2006;Du et al 2009;Lian et al 2012). Highly asymmetric somatic hybrid calli and plants can be produced via symmetric fusion in a tri-parental fusion system in wheat (Li et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Tri-parental protoplast fusion ofBrassicaspecies to produce somatic hybrids with high genetic and phenotypic variability

Lian¹,

Lin²,

Zheng³

2015

Intern. Jour. Contemp. Microbiol.

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Plating efficiencies and the plant regeneration frequencies of bi-parental fusion of Brassica campestris pekinensis with Ogura cytoplasmic male sterility (CMS), B. oleracea L. var. italica with Ogura CMS, and Brassica juncea var. crispifolia were compared for illustrating the advantages of tri-parental somatic hybridization. The results showed that in the triparental fusion combination, 73 plants regenerated from 712 calli and the plant regeneration frequency was 10.3%, three or four times that obtained with the 2 fusion combinations. These hybrids were classified into 12 types based on morphology, and most showed intermediate characteristics between 2 or more of the parental species. The somatic hybiridity were confirmed by flow cytometry, genomic in situ hybridization (GISH) and PCR analysis, indicating that these regenerated plants were all true hybrids. Most of the progenies with normal pollen showed varied number of seed set after backcrossing with B. juncea. The high variability in the hybrids obtained illustrated that somatic hybridization may be useful in broadening existing Brassica gene pools and obtaining material for breeding.

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“…To overcome these poor crossability, several breeding methods have been performed: ovary culture (Luo et al 1989), ovule culture (Dolstra 1982), sequential culture (Lelivelt et al 1993), flower-culture (Metz et al 1995), in vitro organ culture (Luo et al 2000), and protoplast fusion (Pelletier et al 1983, Sakai and Imamura 1990, Lelivelt and Krens 1992, Sakai et al 1996, Wang et al 2006. Furthermore, it has been also difficult to produce backcross progenies in this cross-combination (Dolstra 1982).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

et al. 2009

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In order to breed Brassica napus-Raphanus sativus monosomic addition lines (MALs), hybridizations between two synthetic amphidiploid (RRAA and RRCC) and B. napus (AACC) were performed. Two allooctoploids (RRAAAACC and AACCRRCC) were produced from each F 1 hybrid by chromosome doubling. From successive backcrosses to B. napus, MAL plants were first obtained in BC 2 generation and the R. sativus-derived chromosome was identified by genomic in situ hybridization (GISH). The nine chromosomes of R-genome in the MAL plants were clearly classified by each chromosome-specific RAPD markers. As a result, alloplasmic (radish cytoplasm) B. napus-R. sativus MAL having 8 types (a-i, except for h-type) and autoplasmic (rape cytoplasm) MAL with complete 9 types (a-i) were obtained in BC 3 and BC 4 generations. These alloplasmic and autoplasmic MAL plants were showed differences in their morphological, physiological and cytogenetical characters. From the survey of favorable traits, it was suggested that the a-type had fertility restoring gene(s) for male sterility in alloplasmic line and the g-type had a gene controlling white color petal. These two MALs are useful materials for exploring agronomic traits located on each chromosome of radish and for promoting the introgression of promising radish gene(s) to B. napus.

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Production and characterization of somatic hybrids between Brassica napus and Raphanus sativus

Cited by 22 publications

References 9 publications

Functional Genomics For Crop Improvement

Functional Genomics For Crop Improvement

Tri-parental protoplast fusion ofBrassicaspecies to produce somatic hybrids with high genetic and phenotypic variability

Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"

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