Production and chemical characterization of an exopolysaccharide synthesized by psychrophilic yeast strain Sporobolomyces salmonicolor AL1 isolated from Livingston Island, Antarctica

Abstract

The exopolysaccharide (EPS) production by psychrophilic Antarctic yeast Sporobolomyces salmonicolor AL₁ reached the maximum yield in medium containing sucrose (50 g/L) and diammonium sulfate (2.5 g/L) after a 5-d fermentation (5.64 g/L) at 22 °C, the dynamic viscosity of the culture broth reaching (after 5 d) 15.4 mPa s. EPS showed a mannan-like structure and high molar mass, and did not affect cellular viability and proliferation of murine macrophages. It exhibited also a protective effect against the toxic activity of Avarol.

Psychrophilic yeasts from worldwide glacial habitats: diversity, adaptation strategies and biotechnological potential

“…The Antarctic Peninsula is the northernmost ice‐covered part of the Antarctic mainland: its ecosystem is currently under study to monitor the effects of climate change. Yeasts belonging to the genera Cryptococcus , Mrakia , Leucosporidium and Sporobolomyces (Table ) have quite recently been isolated from soil, moss and lichen (Pavlova et al ., , ; Turkiewicz et al ., , ; Poli et al ., ).…”

“…Likewise, Poli et al . () studied the Antarctic yeast Sporidiobolus salmonicolor (former Sporobolomyces salmonicolor ) as a source of high‐molecular‐mass mannan‐like EPs: maximum yield (5.64 g L −1 ) was achieved at 22 °C.…”

“…The Antarctic Peninsula is the northernmost icecovered part of the Antarctic mainland: its ecosystem is currently under study to monitor the effects of climate Soneda (1961), di Menna (1966b, Goto et al (1969), Cameron (1971), Atlas et al (1978), Baublis et al (1991), Abyzov (1993), Pavlova et al (2009) (Table 1) have quite recently been isolated from soil, moss and lichen (Pavlova et al, 2002(Pavlova et al, , 2009Turkiewicz et al, 2003Turkiewicz et al, , 2005Poli et al, 2010). Some scientific stations are in central Antarctica: due to their harsh climatic conditions, sampling campaigns carried out near these stations are considered to be of paramount ecological interest.…”

Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties – review

“…The inoculum culture of EPS forming strains is carried out in conditions ensuring optimal growth of biomass for 42-48 hours (Cho et al, 2001;Pavlova et al, 2009;Poli et al, 2010), usually in YPD medium. The size of inoculum introduced to the production medium applied in the cited publications was from 1% to 10% v/v.…”

“…Also several strains of R. minuta and Sp. salmonicolor yeasts acidifi ed the culture environment to a value of about 2.0 (Poli et al, 2010). It should be emphasized that the strength of acidifi cation depends on nitrogen source, and is higher in case of inorganic salts application.…”