Production and purification of xylooligosaccharides from oil palm empty fruit bunch fibre by a non-isothermal process

Abstract

h i g h l i g h t sInformation on oligosaccharides production from oil palm biomass is very scarce. Oligosaccharides production from OPEFB fibre via autohydrolysis is demonstrated. Substantial yield of XOS is achieved using non-isothermal conditions. High purity of refined XOS with wide ranges of DP were obtained. a r t i c l e i n f o a b s t r a c tOil palm empty fruit bunches (OPEFB) fibre, a by-product generated from non-woody, tropical perennial oil palm crop was evaluated for xylooligosaccharides (XOS) production. Samples of OPEFB fibre were subjected to non-isothermal autohydrolysis treatment using a temperature range from 150 to 220°C. The highest XOS concentration, 17.6 g/L which relayed from solubilisation of 63 g/100 g xylan was achieved at 210°C and there was a minimum amount of xylose and furfural being produced. The chromatographic purification which was undertaken to purify the oligosaccharide-rich liquor resulted in a product with 74-78% purity, of which 83-85% was XOS with degree of polymerisation (DP) between 5 and 40.

Combined pretreatment with hot compressed water and wet disk milling opened up oil palm biomass structure resulting in enhanced enzymatic digestibility

“…The monomeric and oligomeric glucose, arabinose, galactose and mannose levels were higher from OPFF samples compared to OPEFB probably due to the differences in hemicellulose components, amounts and molecular structures of the fibers. A lower XOS production from OPEFB recorded in this study compared to study reported by Ho et al (2014) probably due to treatment severity applied and a ratio of solid to liquid used.…”

Recent developments in prebiotics to selectively impact beneficial microbes and promote intestinal health

“…Autohydrolysis has also been used to manufacture xylooligosaccharides (XOS) from oil palm empty fruit bunches [41]. This approach produced XOS fractions with varying molecular weight and with low levels of impurities.…”

“…Traditionally, enzymes have been used for the generation of oligosaccharides from plant polysaccharides, but recently there has been interest in the use of autohydrolysis processes [41,42]. This involves treating polysaccharidecontaining material at elevated temperature and pressure.…”

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

“…These results were in accordance with the work of Zakaria et al (2015a), who reported a similar decrease in the pH from 4.10 to 3.39 when the temperature increased from 170 to 210 °C during the hydrothermal pretreatment of oil palm frond fibre (OPFF). The decrease in pH was attributed to the accumulation of acetic acid, which resulted from the cleavage of acetyl groups located in the hemicellulose matrix, and resulted in hemicellulose degradation (Möller et al 2011;Xiao et al 2013;Ho et al 2014).…”