Production and Regulation of Eotaxin-2/CCL24 in a Differentiated Human Leukemic Cell Line, HT93

Yoshida, Naomi; Aizu-Yokota, Eriko; Sonoda, Yoshiko; Moriwaki, Yuji; Kishi, Kenji; Kimura, Tadashi

doi:10.1248/bpb.30.1826

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…If reduced expression of chemokines contributes to immune evasion by colorectal cancer, modulation of chemokine expression in cancer cells could be a possible target for anticancer therapies as well as a prognostic factor for colorectal cancer [ 31 , 32 ]. As some studies on leukemia and CCL24 have suggested, specific kinds of chemokines might affect the migration of specific types of eosinophils in colorectal cancer [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Eosinophils in Colorectal Neoplasms Associated with Expression of CCL11 and CCL24

Cho

Lim²,

Won

et al. 2016

J Pathol Transl Med

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Background:A decrease in the number of tissue eosinophils is known to reflect the malignancy potential of neoplastic lesions and even prognosis. Increased levels of the chemokines CCL11 and CCL24 in serum and tissue are also known to have diagnostic value as serum tumor markers or prognostic factors. The aim of this study was to evaluate the correlation between the degree of tissue eosinophilia and the expression of these chemokines in the glandular and stromal cells of colorectal neoplastic lesions ranging from benign to malignant tumors.Methods:We counted the number of infiltrating eosinophils in neoplastic lesion tissue and we evaluated the expression of CCL11 and CCL24 in glandular cells and stromal cells by immunohistochemical staining.Results:The results showed that the number of eosinophils decreased significantly and the expression of CCL11 and CCL24 in glandular cells decreased with tumor progression, whereas the stromal expression of CCL11 and CCL24 appeared to increase.Conclusions:The discrepancy in CCL11 and CCL24 expression between glandular cells and stromal cells might shed light on how colorectal cancer evades the immune system, which would enable further development of immunotherapies that target these chemokines. Further research on eosinophil biology and the expression pattern of chemokines in tumor cells is needed.

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Section: Discussionmentioning

confidence: 99%

Eosinophils in Colorectal Neoplasms Associated with Expression of CCL11 and CCL24

Cho

Lim²,

Won

et al. 2016

J Pathol Transl Med

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“…Regulation of CCL24 is likely to involve specific transcription factors such as Smad3 and GATA-1. CCL24 was increased in the skin of Smad3-/- mice [ 51 ] and in the GATA-1-overexpressed human leukemic HT93 cell line [ 52 ]. Although TNF induced CCL24 protein in epithelial cells of nasal polyps [ 53 ], it had no effect in SKOV-3 cells [ 5 , 7 ].…”

Section: Discussionmentioning

confidence: 99%

NF-κB-Mediated CCL20 Reigns Dominantly in CXCR2-Driven Ovarian Cancer Progression

et al. 2016

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Ovarian cancer is an inflammation-associated malignancy with a high mortality rate. CXCR2 expressing ovarian cancers are aggressive with poorer outcomes. We previously demonstrated that CXCR2-driven ovarian cancer progression potentiated NF-κB activation through EGFR-transactivated Akt. Here, we identified the chemokine signature involved in CXCR2-driven ovarian cancer progression using a mouse peritoneal xenograft model for ovarian cancer spreading with CXCR2-negative (SKA) and positive (SKCXCR2) cells generated previously from parental SKOV-3 cells. Compared to SKA bearing mice, SKCXCR2 bearing mice had the following characteristics: 1) shorter survival time, 2) greater tumor spreading in the peritoneal cavity and 3) higher tumor weight in the omentum and pelvic site. Particularly, SKCXCR2-derived tumor tissues induced higher activation of the NF-κB signaling pathway, while having no change in EGFR-activated signaling such as Raf, MEK, Akt, mTOR and Erk compared to SKA-derived tumors. Chemokine PCR array revealed that CCL20 mRNA levels were significantly increased in SKCXCR2-derived tumor tissues. The CCL20 promoter activity was regulated by NF-κB dependent pathways. Interestingly, all three κB-like sites in the CCL20 promoter were involved in regulating CCL20 and the proximal region between -92 and -83 was the most critical κB-like site. In addition, SKCXCR2-derived tumor tissues maintained high CCL20 mRNA expression and induced greater CCL24 and CXCR4 compared to SKCXCR2 cells, indicating the shift of chemokine network during the peritoneal spreading of tumor cells via interaction with other cell types in tumor microenvironment. Furthermore, we compared expression profiling array between human ovarian cancer cell lines and tumor tissues based on GEO datasets. The expression profiles in comparison with cell lines revealed that dominant chemokines expressed in ovarian tumor tissues are likely shifted from CXCL1-3 and 8 to CCL20. Taken together, the progression of ovarian cancer in the peritoneal cavity involves NF-κB-mediated CCL20 as a main chemokine network, which is potentiated by CXCR2 expression.

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“…Displayed in our study negative feedback between the expression of eotaxin-2 mRNA and IL-2 and IL-4 may be explained cross-regulation between chemokines and cytokines. Yoshida et al [14] observed that IL-5 does not alter the expression of CCR3 and eotaxin-2 cell line NT93. We found that in healthy volunteers between the expression of eotaxin-2 mRNA and IL-5 production is a negative correlation, which can be attributed to suppressive influence of the Th2 immune response to eotaxin-2.…”

Section: Discussionmentioning

confidence: 98%

Expression of mRNA of chemokines and chemokine receptors and cytokines amount in the blood of healthy volunteers

Сысоев¹

2013

ABB

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Background: Chemokines are small proteins that activate immune system in normal and pathological conditions. The induction of chemotaxis is a well-established role of chemokines. Moreover chemokines are important mediators of angiogenesis, implantation of fetus, and maturation of immune cells. In human body many types of cells express chemokines and cytokines at level of gene and protein. In blood cells chemokine and chemokine receptors mRNA level is a one of crucial points of chemokine system condition. The aim of the study was to evaluate the relationship between plasma concentration of cytokines and chemokines/chemokine receptors mRNA level in blood of healthy volunteers. Results: Gene expression of eotaxin, eotaxin-2, IL-8, MIP-1α, MIP-1β, RANTES, CCR1, CCR3, CCR5, CXCR1, and CXCR2 was measured in peripheral blood cells, as well as the concentration of IL

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Production and Regulation of Eotaxin-2/CCL24 in a Differentiated Human Leukemic Cell Line, HT93

Cited by 8 publications

References 36 publications

Eosinophils in Colorectal Neoplasms Associated with Expression of CCL11 and CCL24

Eosinophils in Colorectal Neoplasms Associated with Expression of CCL11 and CCL24

NF-κB-Mediated CCL20 Reigns Dominantly in CXCR2-Driven Ovarian Cancer Progression

Expression of mRNA of chemokines and chemokine receptors and cytokines amount in the blood of healthy volunteers

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