Production and stabilization of cellulases fromTrichoderma reesei

Illanes, Andrés; Gentina, Juan Carlos; Marchese, Magda

doi:10.1007/bf00940165

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…Although this decrease in enzymatic activity is commonly observed during cellulase production, this phenomenon is not completely understood. Some authors have suggested that the decrease in activity may be due to the effects of a protease on the stability of the cellulases [32], but the presence of inhibitors may also interfere. However, the culture initiated with 0.75% sorbitol and supplemented with cellulose (0.75%) at 12 h after the start of cultivation (Sorb 0.75 + Cel 0.75 (12 h)) reached FPA values of 1.57 ± 0.27 IU.mL −1 .…”

Section: Resultsmentioning

confidence: 99%

Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol

et al. 2013

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The present work investigated the use of sorbitol as a soluble carbon source, in association with cellulose, to produce cellulases and xylanases in submerged cultures of Penicillium echinulatum 9A02S1. Because cellulose is an insoluble carbon source, in cellulase production, there are some problems with rheology and oxygen transfer. The submerged fermentations containing media composed of 0, 0.25, 0.5, 0.75, and 1% (w/v) sorbitol and cellulose that were added at different times during the cultivation; 0.2% (w/v) soy bran; 0.1% (w/v) wheat bran; and a solution of salts. The highest filter paper activity (FPA) (1.95 ± 0.04 IU·mL−1) was obtained on the seventh day in the medium containing 0.5% (w/v) sorbitol and 0.5% (w/v) cellulose added 24 h after the start of cultivation. However, the CMCases showed an activity peak on the sixth day (9.99 ± 0.75 IU·mL−1) in the medium containing 0.75% (w/v) sorbitol and 0.75% (w/v) cellulose added after 12 h of cultivation. The xylanases showed the highest activity in the medium with 0.75% (w/v) sorbitol and 0.25% (w/v) cellulose added 36 h after the start of cultivation. This strategy enables the reduction of the cellulose concentration, which in high concentrations can cause rheological and oxygen transfer problems.

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Section: Resultsmentioning

confidence: 99%

Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol

et al. 2013

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“…Culture medium should have adequate levels of inducer, which can be the substrate, a substrate analogue or the product of the enzyme reaction (Rosenfeld and Feigelson 1969;Kurasawa et al 1992). Substrate analogues are more potent inducers than the substrate itself because they are not acted upon by the enzymes they induce; in the case of depolymerases, inducers are usually intermediate or end products of hydrolysis, since the substrate as such cannot enter the cell to trigger the mechanism (Illanes and Rossi 1981;Illanes et al 1988a). Level and time of addition of the inducer affects the level of enzyme synthesized and are operation parameters that should be optimized .…”

Section: Enzyme Synthesismentioning

confidence: 99%

“…Conditions that maximize the specific cell growth rate are often in compromise with those that maximize the specific rate of enzyme production (Gordillo et al 1998). In fact, it is usual that pH (Illanes et al 1988a;McDermid et al 1988), temperature (Akinrefon 1969;Feller et al 1994) and the level of dissolved oxygen (García-Garibay et al 1987; Barberis and Gentina 1998) optimal for growth differ from the corresponding optima for enzyme production. Compromise values are often used, but impressive increases in enzyme productivity have been reported by profiling these variables during cell culture (Mukhopadhyay and Malik 1980;Mukhopadhyay 1981;Ioniţȃ et al 2001).…”

Section: Enzyme Synthesismentioning

confidence: 99%

Enzyme Production

Illanes

2008

Enzyme Biocatalysis

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“…The rate and extent of cellulose conversion depends, among other things, on the amount of fl-glucosidase activity present in the cetlulase preparation used for efficient saccharification. The most studied fungal source of cellulase, ?>ichoderma reesei, (Illanes et al 1988;Panda 1989) has very little fi-glucosidase activity, which is a disadvantage from the point of view of efficient saccharification. The most studied fungal source of cellulase, ?>ichoderma reesei, (Illanes et al 1988;Panda 1989) has very little fi-glucosidase activity, which is a disadvantage from the point of view of efficient saccharification.…”

mentioning

confidence: 99%

Production and properties ofβ-glucosidase byNeurospora sitophila

Oguntimein

Moo‐Young

1991

World J Microbiol Biotechnol

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Growth at 25°C and pH 5.50 favour the production ofβ-glucosidase. De-fatted oilseed flour and Tween 80 enhanced the production ofβ-glucosidase, Lactose, gentibiose, gentibiose-acetate, laminarabiose and xylobiose inducedβ-glucosidase activity. Precipitation of the culture filtrate with (NH4)2SO4 resulted in 26-fold purification with 67% recovery. The optimum pH and temperature for activity were 5.0 to 5.4 and 55°C respectively. The enzyme was stable at 40°C with half-life at 12 h at 50°C. TheK m andV max for the hydrolysis ofp-nitrophenyl-β-D-glucoside at 40°C H 5.0 are 0.28MM and 0.60 U/mg protein, respectively.

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Production and stabilization of cellulases fromTrichoderma reesei

Cited by 8 publications

References 16 publications

Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol

Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol

Enzyme Production

Production and properties ofβ-glucosidase byNeurospora sitophila

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