Production, Characterization and Application of a Xylanase from<i>Streptomyces</i>sp. AOA40 in Fruit Juice and Bakery Industries

Adıgüzel, Ali Osman; Tunçer, Münir

doi:10.1080/08905436.2016.1199383

Cited by 42 publications

(27 citation statements)

References 51 publications

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“…Xylanases are widely used in various biotechnological applications. Examples include serving as bio-bleaching agents in the pulp and paper industry [4], improving digestibility and enhancing the efficiency of nutrient utilization in animal feed [5,6], increasing dough volume in the bakery industry [7], clarification of juices [8], improving the filtration rate and reduction of viscosity in the brewing industry [9], and releasing xylose that can be fermented to value products, such as biofuels and xylitol [10,11].…”

Section: Introductionmentioning

confidence: 99%

High-Level Heterologous Expression of Endo-1,4-β-Xylanase from Penicillium citrinum in Pichia pastoris X-33 Directed through Codon Optimization and Optimized Expression

et al. 2019

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Most common industrial xylanases are produced from filamentous fungi. In this study, the codon-optimized xynA gene encoding xylanase A from the fungus Penicilium citrinum was successfully synthesized and expressed in the yeast Pichia pastoris. The levels of secreted enzyme activity under the control of glyceraldehyde-3-phosphate dehydrogenase (PGAP) and alcohol oxidase 1 (PAOX1) promoters were compared. The Pc Xyn11A was produced as a soluble protein and the total xylanase activity under the control of PGAP and PAOX1 was 34- and 193-fold, respectively, higher than that produced by the native strain of P. citrinum. The Pc Xyn11A produced under the control of the PAOX1 reached a maximum activity of 676 U/mL when induced with 1% (v/v) methanol every 24 h for 5 days. The xylanase was purified by ion exchange chromatography and then characterized. The enzyme was optimally active at 55 °C and pH 5.0 but stable over a broad pH range (3.0–9.0), retaining more than 80% of the original activity after 24 h or after pre-incubation at 40 °C for 1 h. With birchwood xylan as a substrate, Pc Xyn11A showed a Km(app) of 2.8 mg/mL, and a kcat of 243 s−1. The high level of secretion of Pc Xyn11A and its stability over a wide range of pH and moderate temperatures could make it useful for a variety of biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

High-Level Heterologous Expression of Endo-1,4-β-Xylanase from Penicillium citrinum in Pichia pastoris X-33 Directed through Codon Optimization and Optimized Expression

et al. 2019

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“…Reports on the induction of xylanases from allochthonous bacteria growing in orange processing (by)products are limited in number, while no work on indigenous xylanolytic microbiota isolated from orange juice processing waste exists in the international literature. Streptomyces [40][41][42] and Bacillus/Geobacillus [43,44] spp. isolated from soil are common bacterial strains that have found to exhibit endo-β-1,4-xylanase activities when tested on orange processing (by)products.…”

Section: Resultsmentioning

confidence: 99%

Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste

Zerva

Remmas

Ntougias

2019

Water

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The orange juice processing sector produces worldwide massive amounts of waste, which is characterized by high lignin, cellulose and hemicellulose content, and which exceeds 40% of the fruit’s dry weight (d.w.). In this work, the diversity and the biotechnological potential of xylan-degrading microbiota in orange juice processing waste were investigated through the implementation of an enrichment isolation strategy followed by enzyme assays for the determination of xylanolytic activities, and via next generation sequencing for microbial diversity identification. Intracellular rather than extracellular endo-1,4-β-xylanase activities were detected, indicating that peripheral cell-bound (surface) xylanases are involved in xylan hydrolysis by the examined microbial strains. Among the isolated microbial strains, bacterial isolates belonging to Pseudomonas psychrotolerans/P. oryzihabitans spectrum (99.9%/99.8% similarity, respectively) exhibited activities of 280 U/mg protein. In contrast, almost all microbial strains isolated exerted low extracellular 1,4-β-xylosidase activities (<5 U/mg protein), whereas no intracellular 1,4-β-xylosidase activities were detected for any of them. Illumina data showed the dominance of lactic and acetic acid bacteria and of the yeasts Hanseniaspora and Zygosaccharomyces. This is the first report on indigenous xylanolytic microbiota isolated from orange juice processing waste, possessing the biotechnological potential to serve as biocatalysts for citrus biomass valorization through the production of high-added value products and energy recovery.

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“…Optimum xylanase production by B. pumilus SV‐85S , Bacillus megaterium , and Cellulosimicrobium cellulans CKMX1 was obtained at pH 8.0. Adiguzel and Tuncer obtained maximum xylanase production at pH 9.0 by Streptomyces sp. Very few reports are available in the literature on the production of pectinase under alkaline conditions.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Kaur

Varghese

Mahajan

2019

Biotech and App Biochem

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Simultaneous production of alkaline xylanase and all seven types of pectinases by a bacterial isolate, under solid-state fermentation was checked in this study. Under optimized conditions, high concurrent production of xylanase (22,800 ± 578 IU/g substrate) and pectinase (4,832 ± 189 IU/g substrate) was achieved. The different types of pectinases produced were exo-polymethylgalacturonase (782 IU/g), endo-polymethylgalacturonase (6.42 U/g), exo-polygalacturonase (2,250 IU/g), endo-polygalacturonase (11.57 U/g), polymethylgalacturonate lyase (53.99 IU/g), polygalacturonate lyase (59.78 IU/g), and pectin esterase (5.78 IU/g). Wheat bran resulted in the highest titer of both enzymes. The maximum xylanase-pectinase yield was detected after 7 days of incubation with 2 mM MgSO 4 and 1.5 g/L K 2 HPO 4 at wheat bran to moisture ratio 1:1.5 (w/v), media to flask volume ratio 1:25, pH 7.0, temperature 37 • C, and inoculum size 15%. Xylanase was most stable at pH 8.0, retained more than 75% activity up to 24 H, whereas pectinase was most stable at pH 9.0, having full activity even after 24 H. At 45 • C, the xylanase showed 82% residual activity after 6 H of incubation. The pectinase was 97% and 61% stable up to 3 H at 50 and 55 • C, respectively. This is the first report showing the production of xylanase-pectinases by bacterium along with high titer of seven types of pectinases, suitable for industries.

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Production, Characterization and Application of a Xylanase fromStreptomycessp. AOA40 in Fruit Juice and Bakery Industries

Cited by 42 publications

References 51 publications

High-Level Heterologous Expression of Endo-1,4-β-Xylanase from Penicillium citrinum in Pichia pastoris X-33 Directed through Codon Optimization and Optimized Expression

High-Level Heterologous Expression of Endo-1,4-β-Xylanase from Penicillium citrinum in Pichia pastoris X-33 Directed through Codon Optimization and Optimized Expression

Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

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