Production of a sheep transgenic with the ovine growth hormone gene

Nancarrow, C. D.; Marshall, J.; Hazelton, IG; Ward, Kevin

doi:10.1016/0093-691x(87)90140-3

Cited by 9 publications

(5 citation statements)

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“…1985 ;Vize et al . 1988) and sheep (Hammer et al 198%;Nancarrow et al 1987) and MT -GHRF into sheep (C. Rexroad, personal communication) has been reported. However, the genetic manipulation of a complex physiological trait such as growth is fraught with complications.…”

Section: Animal Improvementmentioning

confidence: 86%

The molecular manipulation of milk composition

Clark¹,

Ali²,

Archibald³

et al. 1989

Genome

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The introduction of cloned genes into the mouse germ line is now routine. Although more difficult technically, gene transfer has been accomplished in farm animals and offers the potential for genetic improvement. In this regard, we have been investigating the use of transgenic animals as production vehicles for high value proteins in milk. We have shown that DNA sequences derived from the gene encoding sheep beta-lactoglobulin mediate efficient and specific expression in the mammary gland. A fusion gene comprising beta-lactoglobulin sequences and those encoding antihemophilic human factor IX has been constructed. This construct has been introduced into sheep; it is expressed in the mammary gland, and the corresponding protein is secreted into milk.

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Section: Animal Improvementmentioning

confidence: 86%

The molecular manipulation of milk composition

Clark¹,

Ali²,

Archibald³

et al. 1989

Genome

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“…From the data shown above it can be concluded that the maximal overall efficiency in producing transgenic animals is approximately 4% in mice (33,71), 3% in rabbits, (116) 1% in pigs (114) and 0.2% in sheep (117). In order to produce transgenic farm animals for research purposes this efficiency has to be increased drastically considering the expenses involved but until now hardly any attention has been paid to transgenic production efficiencies and little is known about the limiting factors.…”

Section: Factors Limi Ting the Production Of Transgenic Livestockmentioning

confidence: 94%

“…Up to the present the production of transgenic rabbits (113)(114)(115)(116), sheep (114,117), pigs (113,114) and chicken (118) has been reported. Attempts to produce transgenic goats (119) and cattle (120-122) have not so far been successful.…”

Section: Attempts To Produce Transgenic Livestockmentioning

confidence: 98%

“…It can be assumed that genetic and species-specific conditions can cause different limitations of gene integration efficiency, an assumption that is supported by the fact that in sheep integration frequency is comparatively low (114,117) and also by the observation that integration frequency varies considerably between different mouse strains (5) and somatic cell lines of different species (127). Therefore, the maj or limitations of gene transfer efficiency into the germline of animals appear to arise from deleterious effects of the injected gene suspension on the viability of the manipulated embryos and from low integration frequencies.…”

Section: Factors Limi Ting the Production Of Transgenic Livestockmentioning

confidence: 99%

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The Use of Transgenic Animal Techniques for Livestock Improvement

Strojek

Wagner

1988

Genetic Engineering

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“…The transfer of recombinant DNA into animals by micro injection into the pronuclei of one-cell embryos has been demonstrated in mice, pigs, rabbits and sheep (Hammer et al 1985;Brem et al 1986;Palmiter and Brinster 1986;Pursel et al 1986;Nancarrow et at. 1987).…”

Section: Introductionmentioning

confidence: 99%

The Isolation and Characterisation of the Ovine Growth Hormone Gene

Byrne¹,

Wilson²,

Ward³

1987

Aust. Jnl. Of Bio. Sci.

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The ovine growth hormone gene has been isolated and sequenced, together with about 1 kbp of DNA flanking each end of the gene. The structure of the gene is similar to that found for other growth hormone genes, particularly the bovine gene, and has a primary transcript of 1792 bp, with five exons, and with intron sizes of 264 bp, 231 bp, 227 bp and 273 bp.The gene is flanked by artiodactyl-specific middle-repetitive DNA, consisting mainly of elements belonging to the 'C-A3' family of repeated DNA. A previously unreported sequence has been found, which we have named an 'E' element.

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Production of a sheep transgenic with the ovine growth hormone gene

Cited by 9 publications

References 0 publications

The molecular manipulation of milk composition

The molecular manipulation of milk composition

The Use of Transgenic Animal Techniques for Livestock Improvement

The Isolation and Characterisation of the Ovine Growth Hormone Gene

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