Production of an endopolygalacturonase from Wickerhanomyces anomalus with disintegration activity on plant tissues

Pectinases Secretion by Saccharomyces cerevisiae: Optimization in Solid-State Fermentation and Identification by a Shotgun Proteomics Approach

“…The low value for the peptone concentration significance was, to some extent, unexpected as the reports found in the literature strongly suggested nitrogen supplementation as an important variable for large scale enzyme production: Arévalo-Villena et al [ 35 ]; Kaur et al [ 36 ] and Maidana et al [ 6 ] concluded that the addition of yeast extract, peptone, ammonia or urea to agro-industrial residue media significantly increased the levels of secreted enzymatic activity by yeasts. To better evaluate the effect of peptone supplementation of the culture medium, for the subsequent experimental design, the maximum peptone content tested was doubled.…”

“…Although pectinases are produced by plants and microorganisms, there is a greater interest in pectinases secreted by the latter. Some advantages of the microbial sources are the possibility of secretion induction by cultivation media, oriented biosynthesis with specific catalytic sites and large-scale production associated with yield optimization and productivity, regardless of seasonal factors or high-cost substrate [ 3 , 5 , 6 ]. Yeasts have drawn attention in the past years as a consequence of the diversity of pectinases that can be produced by this fungal group and their biochemical plasticity, simplifying cultivation and handling [ 1 , 7 ].…”

Use of Agro-Industrial Wastes for the Production of a Wild Yeast Enzyme with Disintegration Activity on Plant Tissues

“…Maidana and coauthors reported a maximum production of PGase by W. anomalus of~25.52 UEmL -1 in a medium composed of (gL -1 ): glucose, commercial citrus pectin, urea, yeast extract, KH2PO4, CaCl2 and MgSO4, in a batch system, at a lab scale bioreactor [19]. In this study it was obtained a highest production of the enzyme (31.28 ± 0.93 EUmL -1 ), in an economical culture medium and using an agro-industrial waste.…”

“…The significant factors of the PB design were further optimized using response surface method (RSM) [38]. A design proposed by Doehlert was selected for studying the response surface [18,19,39]. The real values of the independent variables were coded based on a linear functionality between codified (Z) and actual values (X) according to:…”

“…The design of Plackett-Burman is a fractional factorial design, very useful for preliminary studies where a large number of variables must be investigated and in which the use of a complete factorial model becomes impractical due to the large number of required tests. Response surface methodology (RSM) involves full factorial search by examining simultaneous, systematic and efficient variation of all components [13,[17][18][19].…”