Production of Avermectin B1b From Streptomyces avermitilis 41445 by Batch Submerged Fermentation

Siddique, Samia; Syed, Quratulain; Adnan, Ahmad; Nadeem, Muhammad; Irfan, Muhammad; Qureshi, Fahim Ashraf

doi:10.5812/jjm.7198

Cited by 18 publications

(15 citation statements)

References 28 publications

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“…Compounds derived from avermectin that show great biological activity as anthelmintic agents include abamectin, doramectin, ivermectin and selamectin. In particular, abamectin is a mixture constituted roughly of 80% of avermectin B1a and 20% of avermectin B1b [19]. Avermectins block the γ-amino butyric acid stimulated chloride channels and open non-neurotransmitter-gated chloride channels [20], causing an ion imbalance in the nervous system, resulting in paralysis.…”

Section: Introductionmentioning

confidence: 99%

Abamectin Efficacy on the Potato Cyst Nematode Globodera pallida

Sasanelli

Toderaş

Veronico

et al. 2019

Plants

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The potato cyst nematode Globodera pallida is a major pest of the potato crop. Abamectin is a biological pesticide showing high nematicide activity, but its efficacy to control G. pallida has not been investigated to date. In this study, combination of different abamectin concentrations ranging from 1.125 to 36 µg/mL x exposure times from 24 to 384 h were tested on the nematode in a hatching test. Abamectin induced mortality with LD50 value in the range of 13.23 (after 24 h) to 2.90 µg/mL (after 384 h). A glasshouse experiment was also performed in pots filled with soil infected with G. pallida in the presence of sprouted potato tubers cultivar “Spunta”. Abamectin at 4.5, 9.0, 18.0 and 36.0 µg/mL was used in comparison with nematicide fosthiazate. The doses of 18 and 36 µg/mL significantly reduced number of eggs, juveniles, cyst/g soil and reproduction rate in comparison to both untreated control and fosthiazate treatment. Soil applications of abamectin provided significant G. pallida control with LD50 and LD99.9 of 14.4 and 131.3 µg/mL, respectively. These results indicate the efficacy of abamectin to control G. pallida on potato crops and its potential use in organic agriculture or in an integrated pest management program.

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Section: Introductionmentioning

confidence: 99%

Abamectin Efficacy on the Potato Cyst Nematode Globodera pallida

Sasanelli

Toderaş

Veronico

et al. 2019

Plants

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“…These approaches have been applied to improve avermectin production in the fermentation industry and increased the titer to 0.5 g/L by strain selection from ultraviolet (UV) light radiation, Methyl methanesulphonate (MMS) and N-methyl-N-nitro-N-nitrosoguanidine (NTG) treatment and media modifications. 8 , 9 …”

Section: Improving the Production Of Avermectin By Traditional Mutagementioning

confidence: 99%

“…10 , 12 , 13 Moreover, a B1a ratio increase by glucose feeding was observed, and the stimulation is further enhanced by controlled glucose feeding. 7 , 9 It has been suggested that glucose affects avermectin formation by providing additional dTDP-oleandrose, an immediate precursor of avermectin. 14 , 15 The B1a ratio might be changed due to the feeding of glucose, which could regulate the activity of aveD , a crucial gene that is responsible for the conversion between avermectin B and A types.…”

Section: Improving the Production Of Avermectin By Traditional Mutagementioning

confidence: 99%

Interrogation of Streptomyces avermitilis for efficient production of avermectins

Chen

Liu

et al. 2016

Synthetic and Systems Biotechnology

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The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin, respectively. Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents. Because wild-type strains only produce low levels of avermectins, much research effort has focused on improvements in avermectin production to meet the ever increasing demand for such compounds. This review describes the strategies that have been widely employed and the future prospects of synthetic biology applications in avermectin yield improvement. With the help of genome sequencing of S. avermitilis and an understanding of the avermectin biosynthetic/regulatory pathways, synthetic and systems biotechnology approaches have been applied for precision engineering. We focus on the design and synthesis of biological chassis, parts, devices, and modules from diverse microbes to reconstruct and optimize their dynamic processes, as well as predict favorable effective overproduction of avermectins by a 4Ms strategy (Mine, Model, Manipulation, and Measurement).

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“…are one of the most important actinobacteria which can provide new bioactive compounds for use as insect-control agents, such as avermectin, doramectin and quinomycin (Huamei et al, 2008 andKorkmaz et al, 2015). abamectin (aBM), a macrocyclic lactone natural product extracted from Streptomyces avermitilis, has been documented most recently as a bio-insecticide in many parts of the world (Burg et al, 1979;Fisher & Mrozik, 1989;Mujica et al, 2000;Mahmoud et al, 2013 andsiddique et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

Mahfouz

Barakat

2018

Egypt. J. Bot.

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E sseNtial oils and the bio-insecticides have been utilized to protect the plants aginst different kind of insects. However, it is really improtent to study the side effects of these essential oils and insecticides on plants. the iPBs technique based on long terminal Repeat (ltR) retrotransposons was applied in this study to detect the DNa damage in cowpea seeds exposed to three plant oils of Trigonella foenum-graecum, Panax ginseng, Origanum majorana and the bio-insecticide abamectin. Different levels of polymorphism ranged from 37.50% polymorphism for primer 2394 to 82.35% polymorphism for primer 2270 with a total average of 52.00%. also, decrease in genetic template stability (Gts%) values were recorded with all treatments as compared with the control. the value of Band sharing index (Bsi) for each treatment was calculated and the results showed that mixture 2 (lC20 abamectin + 2lC95 ginseng) showed the lowest (Bsi) as compared with all treatments. the obtained results, suggested that the assurance should be strongly taken in the consideration for the safety, efficacy, and toxicity of the preparation derived from plants. Furthermore, iPBs assay is a powerful tool for the detection of the genotoxic effects of environmental chemicals and offer great promise for future especially for the determination of genetic damage following exposure to contaminants.

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Production of Avermectin B1b From Streptomyces avermitilis 41445 by Batch Submerged Fermentation

Cited by 18 publications

References 28 publications

Abamectin Efficacy on the Potato Cyst Nematode Globodera pallida

Abamectin Efficacy on the Potato Cyst Nematode Globodera pallida

Interrogation of Streptomyces avermitilis for efficient production of avermectins

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

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