Production of Bioethanol from the Hydrolysate of Brown Seaweed (Sargassum crassifolium) using a Naturally β-glucosidase Producing yeast Saccharomyces cereviceae JCM 3012

Widyaningrum, Trianik; Prastowo, Indro; Parahadi, Masreza; Prasetyo, Angga

doi:10.13005/bbra/2274

Cited by 18 publications

(8 citation statements)

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“…PDA yang sudah disterilisasi dimasukkan dalam tabung reaksi sebanyak 5 mL, lalu dinginkan dengan memposisikan tabung miring. Kemudian mengkulturkan jamur menggunakan ose bulat dan diinkubasi dalam inkubator dengan suhu 37⁰C selama 24 jam (Widyaningrum et al, 2017).…”

Section: Penumbuhan Trichoderma Reesei Dan Aspergillus Nigerunclassified

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis

Widyaningrum,

Rizqiyah

2023

Bioscientist j. ilm. bosbiol.

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The need for fuel oil in Indonesia is increasing along with the increase in the number of people's consumption in the fields of transportation, industry, and economy. While the source of petroleum fuel in Indonesia is running low. Therefore, it is necessary to utilize alternative energy, namely bioethanol. Bioethanol can be produced from organic waste that contains high cellulose, one of which is peanut shells. This study aims to determine the effect of the ratio of crude cellulase enzyme Trichoderma reesei and Aspergillus niger on sugar and ethanol content of peanut shell fermentation using Zymomonas mobilis, and to determine the ratio of crude cellulase enzyme that produces the highest sugar and ethanol content of peanut shell fermentation using Z. mobilis. This research is an experimental study with independent variables, namely the ratio of crude cellulase enzyme T. reesei : A. niger (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) and the dependent variable of sugar and biethanol content. Data analysis of experimental research results using ANOVA and DMRT test. The addition of crude cellulase enzyme from T. reesei and A. niger affects the sugar content and bioethanol from peanut shell fermentation. The ratio of crude cellulase enzyme from T. reesei and A. niger that produces the highest sugar content is in the treatment P5 ratio of crude enzyme T. reesei: A. niger (2:1) which is 0.14 g/mL and the highest ethanol content from groundnut shell fermentation is in the treatment of P8 crude enzyme ratio of T. reesei : A. niger (1:3) which is 2.23%.

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Section: Penumbuhan Trichoderma Reesei Dan Aspergillus Nigerunclassified

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis

Widyaningrum,

Rizqiyah

2023

Bioscientist j. ilm. bosbiol.

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“…The anthocyanin content was determined according to Veazie et al (2020) using a pH differential method at 520 and 700 nm, and expressed as a mg of cyanidin-3-glucoside equivalent (CGE) per gram of sample.…”

Section: Anthocyanin Contentmentioning

confidence: 99%

Production of yoghurt with Clitoria ternatea flower extract supplementation, and its stability during storage

Prastowo,

Sundari,

Hanifah

et al. 2023

IFRJ

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The aim of the present work was to analyse 24 h yoghurt fermentation supplemented with Clitoria ternatea flower (CTF) extracts (0 - 10%); especially elucidating the relationship between antioxidant activity, carbohydrate constituent, and microbial growth which has never been reported. Carbohydrate constituent in the CTF was also investigated for the first time. Colour changes was also assessed during yoghurt production. Furthermore, the stability of yoghurt was studied during the 7 d storage under low temperature (4°C). The supplementation of CTF extracts (0 - 10%) into yoghurt increased the antioxidant activity (up to 46.65 ± 0.29%) and carbohydrate concentration (glucose, up to 9.63 ± 0.3%; sucrose, up to 7.8 ± 0.5%; inulin, up to 5.7 ± 0.8%; and pectin, up to 7.5 ± 0.3%), but decreased dissolved oxygen (DO) down to 0.65 ± 0.023 mg/L in the medium during fermentation. Surprisingly, prebiotic sugars of inulin and pectin were discovered in CTF. The presence of higher carbohydrate concentration and more anaerobic condition enabled Lactobacillus delbrueckii subsp. bulgaricus to grow up to 7.74 ± 0.1 log CFU/mL. In contrast, the final cell concentration of Streptococcus thermophillus decreased up to 8.12 times as the extract concentrations increased. However, the viability of both bacteria still met the international standards (≥ 7 log CFU/mL). The yoghurt colour turned from light turquoise to purple (L* = 69.47 ± 0.2; a* = 14.78 ± 0.15; b* = - 21.77 ± 0.2) as the pH decreased to 4.5 ± 0.11, and the lactic acid concentration increased up to 1.74 ± 0.37%. Furthermore, the quality of yoghurt in all parameters was relatively stable during storage for antioxidant activity, microbial growth, carbohydrate constituent, DO, lactic acid concentration, anthocyanin content, and pH; meanwhile colour changes only decreased 0 - 0.39 times.

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“…Setelah dingin ditambahkan crude enzim dari A. niger dan T. reesei sebanyak 10 mL dengan variasi K (0:0), P1 (1:0), P2 (0:1), P3 (1:1), P4 (1:2), P5 (2:1), P6 (3:1) dan P7 (1:3) dengan tiga kali ulangan (24 botol). Botol ditutup menggunakan kapas steril, dilapisi alumunium foil dan diikat kemudian diinkubasi selama 1x24 jam dengan mengatur suhu ruangan dengan suhu 37 o C. Hasil hidrolisis diukur menggunakan metode DNS (Dinitrosalicylic acid) untuk mengetahui kadar gula (Widyaningrum dan Parahadi, 2020b).…”

Section: Hidrolisisi Enzimatikiunclassified

Pengaruh Rasio Crude Enzim Aspergillus niger dan Trichoderma reesei terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Buah Nangka (Artocarpus heterophyllus Lamk.)

Pertiwi¹,

Widyaningrum²

2022

metamorfosa

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Bioetanol adalah etanoliyangiberasal dari sumberihayatiidanidapatidigunakanisebagaii bahanibakari alternatif.i Bioetanol dapat dibuat dari bahan yang mengandung selulosa, misalnya kulit buah nangka yang mengandung selulosa 38,69% v/v. Penelitian ini bertujuan untuk mengetahui pengaruh perbandingan crude. enzim. A.. nigeri dani T.iireesei iterhadap ikadar igula idan bioetanolihasilifermentasi kuliti buah nangka menggunakan Z. mobilis. Jenis penelitiani inii adalah penelitianieksperimen. Rancangani percobaan imenggunakan iRAL dengani variabeli bebasi rasioicrudeiA. niger dan T. reesei dengan varian (0:0), (0:1), (1:0), (1:1), (1:2), (2:1), (1:3), dan i(3:1). Variabeliterikat berupa kadari igula dani ibioetanoli hasiliifermentasi kuliti buah nangka. Kadari gula diukur dengani imetode iDNS. Kadari etanol diukur menggunakan ialkoholmeter. Datai eksperimen dianalisis dengan SPSS uji ANOVA. Hasil penelitian menunjukkan bahwa rasioi crudei enzimi A. nigeri dani IT. reesei berpengaruh terhadapi kadari gulai dani bioetanoli ifermentasi ikulit buah nangka menggunakan Z. mobilis. Kadar gula tertinggi pada rasio crude enzim A. niger : T. reesei 1:0 sebesar 11,99 % v/v dan kadar etanol tertinggi pada rasio crude 2: 1 yaitu 3,16 % v/v.

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Production of Bioethanol from the Hydrolysate of Brown Seaweed (Sargassum crassifolium) using a Naturally β-glucosidase Producing yeast Saccharomyces cereviceae JCM 3012

Cited by 18 publications

References 0 publications

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis

Production of yoghurt with Clitoria ternatea flower extract supplementation, and its stability during storage

Pengaruh Rasio Crude Enzim Aspergillus niger dan Trichoderma reesei terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Buah Nangka (Artocarpus heterophyllus Lamk.)

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