Production of Biopharmaceuticals in Microalgae

Bañuelos-Hernández, Bernardo; Beltrán-López, Josué I.; Rosales‐Mendoza, Sergio

doi:10.1016/b978-0-12-800776-1.00018-2

Cited by 12 publications

(10 citation statements)

References 66 publications

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“…Moreover, algae are capable of performing post-translational modifications (e.g., glycosylation in endoplasmic reticulum and Golgi) that are frequently important for the antigen activity, can be produced relatively fast and some species could be used to formulate vaccines in a straightforward manner since they hold a GRAS status (Specht and Mayfield, 2014 ). This notion has been primarily explored for the freshwater microalga Chlamydomonas reinhardtii with important advances toward the development of low cost orally-delivered vaccines (Bañuelos-Hernández et al, 2015 ; Rasala and Mayfield, 2015 ; Dyo and Purton, 2018 ). Two interesting reports in this area comprise a developed S. aureus vaccine based on C. reinhardtii , showing antigen yields up to 0.7% TSP.…”

Section: Microalgae-made Vaccinesmentioning

confidence: 99%

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega¹,

Rosales‐Mendoza

Bañuelos-Hernández

et al. 2018

Front. Microbiol.

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Although oral subunit vaccines are highly relevant in the fight against widespread diseases, their high cost, safety and proper immunogenicity are attributes that have yet to be addressed in many cases and thus these limitations should be considered in the development of new oral vaccines. Prominent examples of new platforms proposed to address these limitations are plant cells and microalgae. Schizochytrium sp. constitutes an attractive expression host for vaccine production because of its high biosynthetic capacity, fast growth in low cost culture media, and the availability of processes for industrial scale production. In addition, whole Schizochytrium sp. cells may serve as delivery vectors; especially for oral vaccines since Schizochytrium sp. is safe for oral consumption, produces immunomodulatory compounds, and may provide bioencapsulation to the antigen, thus increasing its bioavailability. Remarkably, Schizochytrium sp. was recently used for the production of a highly immunoprotective influenza vaccine. Moreover, an efficient method for transient expression of antigens based on viral vectors and Schizochytrium sp. as host has been recently developed. In this review, the potential of Schizochytrium sp. in vaccinology is placed in perspective, with emphasis on its use as an attractive oral vaccination vehicle.

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Section: Microalgae-made Vaccinesmentioning

confidence: 99%

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega¹,

Rosales‐Mendoza

Bañuelos-Hernández

et al. 2018

Front. Microbiol.

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“…Microalgae synthesize all 20 proteinogenic amino acids and can be unconventional sources of essential amino acids for human nutrition (82). Recombinant DNA and hybridoma technologies made it possible to produce, at large scale, proteins acting as drugs, which are referred to as drugs, biologics, or biopharmaceuticals (BFs) (83). In past, potential vaccine candidates have been produced by viruses and bacteria as well as malaria and other communicable diseases or have been investigated for non-viral diseases (84,85).…”

Section: Proteins and Polypeptidementioning

confidence: 99%

Microalgae as a source of high-value bioactive compounds

et al. 2018

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Microalgae are one of the oldest microorganisms, that grow in various hostile environments, ranging from deserts to Antarctica. The microalgae sustain life in such harsh environments through generation of secondary metabolites. Microalgae biosynthesize a large number of diverse bioactive metabolites with activities on cancer, neurodegenerative diseases, and infectious diseases. Here, we highlight the bioactive compounds that are isolated from microalgae for the purpose of using them as food, and as chemicals in pharmaceutical industry as new agents with therapeutic benefits.

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“…By growing in aqueous solutions of high salinity, marine microalgae N. salina has been used extensively to produce biofuels, 26 pharmaceuticals, 27 and fish feed 28 . Many studies have been conducted to propose mathematical models for predicting microalgal growth and product formation 29,30 .…”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23][24] By effectively converting the electrical energy into light energy, 24 the light-emitting diodes (LEDs) would be able to not only supply photons at desired wavelengths to be effectively adsorbed by microalgae, but also with appropriate intensity to support their phototropic growth. 25 By growing in aqueous solutions of high salinity, marine microalgae N. salina has been used extensively to produce biofuels, 26 pharmaceuticals, 27 and fish feed. 28 Many studies have been conducted to propose mathematical models for predicting microalgal growth and product formation.…”

mentioning

confidence: 99%

Kinetic models to describe Nannochloropsis salina cultivation under different illumination conditions

Shirazian

Azin

Bozorg

2022

Env Prog and Sustain Energy

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Nannochloropsis salina (N. salina) has recently received growing interests as a sustainable source of high‐value bioproducts. As the illumination condition could be manipulated to affect the microalgae activities in indoor cultivation, in this study, kinetic models were developed to describe the growth and bioproduct formation in N. salina under different illumination conditions. Being continuously exposed to white (380–760 nm), blue (430 nm), red (680 nm), and mixed blue/red light, the growth of N. salina was assessed over the course of 12 days incubation and the evolving cell densities, lipid, and carbohydrate concentrations were recorded. Kinetic models were then used to fit the experimental results and describe microalgae behaviors with respect to illumination conditions. The highest growth rates were recorded when the microalgae were exposed to the blue/red light, leading to 0.8 g/L standing dry biomass. Also, although lipid and carbohydrate productions were stimulated under other illumination conditions, the advanced biomass production achieved by blue/red light resulted in the highest lipid and carbohydrate productions. As confirmed by the kinetic models, the results obtained in this study revealed that the light absorbed by the photosynthetic pigments of N. salina could be manipulated to not only control the microalgae growth, but also its biological activities, including carbohydrate and lipid productions. Therefore, a two‐stage process would be recommended for lipid and carbohydrate production by N. salina.

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Production of Biopharmaceuticals in Microalgae

Cited by 12 publications

References 66 publications

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Microalgae as a source of high-value bioactive compounds

Kinetic models to describe Nannochloropsis salina cultivation under different illumination conditions

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