Production of doubled haploids in durum wheat (Triticum durum Desf.) through culture of unpollinated ovaries

Abstract

The objective of this work was to produce doubled haploid plants from durum wheat through gynogenesis using unpollinated ovary culture of three local Tunisian genotypes (Jenah Khotifa, Hmira, Azizi) and three improved cultivars (Karim, Khiar, Razzek). A total of 12,000 unpollinated ovaries were cultured in this study. Spikes were either pretreated at 4°C for 14 days or at 4°C in a mannitol solution (0.3 M) for 7 days. Induction was performed using two media. We showed that ovary development, callus and plantlet regeneration was influenced significantly by genotype and growth conditions. The highest regeneration frequency was obtained when the microspore population was in the late mononucleate to binucleate stage. Our results suggested that the cold pretreatment for 14 days was more efficient than the cold treatment in a mannitol solution. Furthermore, the addition of 2,4-D, vitamins and glutamine, and the use of maltose as sugar source in media improved the ovary culture. When the unpollinated ovaries were cultured under the conditions found to be optimal in the present study, a total of 84 plants were produced, all green and haploid.

Selection of Tolerant Lines to Salinity Derived from Durum Wheat (<i>Triticum durum</i> Desf.) in Vitro Culture

“…Gynogenesis was tested for durum wheat genotype Jenah Khotifa (JK) and was responsive and produced calli, regenerated plantlets, green haploid plant. Rates of haploid plant regenerated were acceptable comparatively to previous studies with durum wheat ( [10] [15] [16]). This tehnique offers great potential and has the advantage that all the haploid plants obtained are green.…”

DEVELOPMENT OF HAPLOID PLANTS THROUGH OVARY CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L ).

“…2, 4-D was affected the cultivars gynogenesis abilities (Madarhri-Alaoui et al, 1998 andAlaoui et al, 2005). Gynogenesis is variety dependant, many researchers were in agreement with this (Erezhenov et al, 1991;Alaoui et al, 2005;Salama-Ayed andSlim Amara, 2007 andAyed et al, 2010). So most researchers directed to the distant hybridization via chromosome elimination methods.…”

Relative efficiency of maize and Imperata cylindrica for haploid induction in Triticum durum following chromosome elimination‐mediated approach of doubled haploid breeding

“…), unpollinated ovary culture (Sibi et al. , Slama‐Ayed and Slim‐Amara ) and using wide hybridization with wild barley Hordeum bulbosum L. (O'Donoughue and Bennett , Chlyah et al. ), pearl millet (Inagaki and Hash ) and maize (O'Donoughue and Bennett , Almouslem et al.…”