Production of extracellular bifidogenic growth stimulator by anaerobic and aerobic cultivations of several propionibacterial strains

Kouya, Tomoaki; Misawa, Katsuhiro; Horiuchi, Masahito; Nakayama, Eri; Deguchi, Hiroyoshi; Tanaka, Takaaki; Taniguchi, Masayuki

doi:10.1263/jbb.103.464

Cited by 33 publications

(28 citation statements)

References 17 publications

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“…al., , 2001(Taniguchi et. al., , 2005Kouya et al, 2007). As NPSI 38 consumed glucose gradually in the media, only lactic acid was produced corresponding to the consumption of glucose, and no other organic acids were detected throughout the cultivations, indicating that NPSI 38 is a typical homo-fermentative LAB.…”

Section: Resultsmentioning

confidence: 94%

Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria

Ishiyama

Takata

Nakanishi

et al. 2008

FSTR

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Enhanced production of bacteriocin by Staphylococcus sp. NPSI 38 (NPSI 38) using koji extract medium was investigated. The bacteriocin produced by NPSI 38 in MRS medium was found to be effective for inhibiting the growth of Lactobacillus hilgardii NBRC 15886 T , one of the representative hiochi-bacteria. In cultivations using test tubes without pH control, meat extract and Polypepton were effective for bacteriocin production by NPSI 38 with 10%(v/v) koji extract medium. When the koji extract medium supplemented with hydrolyzate of a rice protein preparation was used instead of meat extract and Polypepton, NPSI 38 produced 160 U/ml of bacteriocin in the cultivation with pH control, which was almost as high as that (156 U/ml) observed in cultivation using MRS medium with pH control. When the cells of L. hilgardii NBRC 15886T collected at logarithmic growth and stationary phases were inoculated into fresh modified MRS medium with 11 U/ml of the bacteriocin, negligible cell growth was observed, irrespective of different growth phases of cells inoculated. Little or no increase in cell concentrations in the media containing bacteriocin showed that the action of the bacteriocin produced by NPSI 38 was bacteriostatic against the hiochi-bacterium.Keywords: bacteriocin, hiochi-bacteria, Lactobacillus hilgardii, koji extract medium, rice protein *To whom correspondence should be addressed. Email: mtanig@eng.niigata-u.ac.jp IntroductionHiochi-bacteria are recognized as major potential spoilage microorganisms of sake. Hiochi-bacteria have been identified as several Lactobacillus species (Lactobacillus fructivorans, Lactobacillus paracasei subsp. paracasei, Lactobacillus plantarum and Lactobacillus hilgardii) by molecular biology and biochemistry methods (Goto-Yamamoto et al., 1994, Niidome et al., 1995. Hiochi caused by outbreaks of the bacteria results in lowering of the pH, occurrence of turbidity, and formation of off-flavors such as diacetyl. The spoilaging phenomena seriously deteriorate the quality of sake. To prevent hiochi from emerging in the sake brewing industry, pasteurization (hiire) at around 65℃ for a short duration of 2-3 min or microfiltration for removing hiochibacteria has been carried out in the sake brewing process.However, the spoilage of sake caused by hiochi-bacteria is not completely repressed at present.A large number of bacteriocins, antimicrobial peptides, are produced by many lactic acid bacteria (LAB) (Onda et al., 2002, Soriano et al., 2004, Sparo et al., 2006. Since bacteriocins are isolated from foods, such as meat and dairy products, which normally contain LAB, they have unknowingly been consumed for centuries by humans (Goktepe, 2006). Thus, bacteriocins from LAB have been shown to be safe, and have the potential to be effective as natural food preservatives (Ennahar et al., 2000, Cleveland et al., 2001. Many bacteriocins are small proteins, stable at high temperature, digested by protease (Ray, 1992, Heng et al., 2007, and only effective against a limited narrow spectrum of bacteria, typic...

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Section: Resultsmentioning

confidence: 94%

Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria

Ishiyama

Takata

Nakanishi

et al. 2008

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“…This result implies that Bifidobacterium strains may produce a growth-promoting substance for EAggEC which is effective at neutral pH. Kouya et al [31] reported that several Propionibacterium strains produced an extracellular growth-stimulating substance for Bifidobacterium. Like the growth-promoting effect exerted by Bifidobacterium strains on EAggEC presented here, the growth-promoting activity exerted by Propionibacterium was only detected at pH 6.5-8.5.…”

Section: Discussionmentioning

confidence: 92%

Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli

Kamiya

Hanawa

et al. 2010

Journal of Infection and Chemotherapy

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“…Determination of the Inhibitory Effect of Culture Filtrate of Propionibacterial Strains on the Growth of H. pylori Propionibacterium acidipropionici JCM 6432 and P. jensenii JCM6433 were incubated in TPY medium as described previously [27], and culture filtrates of propionibacterial strains were prepared from the culture broths obtained after incubation for 48 hour. About 10 6 H. pylori cells were inoculated in a plate of Brucella agar (Becton Dickinson) containing 5% horse serum and 0.005% 2,3,5-triphenyltetrazorium chloride (Wako Pure Chemicals), and then a 200 lL of each culture filtrate of the propionibacterial strain, TPY medium, and 3 lg ⁄ mL of DHNA were added to a dish (5 mm in diameter) in the center of the Brucella agar plate.…”

Section: Determination Of Mic Of Dhna and Clarithromycinmentioning

confidence: 99%

The Bifidogenic Growth Stimulator Inhibits the Growth and Respiration of Helicobacter pylori

Nagata

Inatsu

Tanaka³

et al. 2010

Helicobacter

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Production of extracellular bifidogenic growth stimulator by anaerobic and aerobic cultivations of several propionibacterial strains

Cited by 33 publications

References 17 publications

Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria

Production of Bacteriocin by Staphylococcus sp. NPSI 38 in Koji Extract Medium with Rice Protein Hydrolyzate and Its Growth-inhibitory Activity against Hiochi-bacteria

Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli

The Bifidogenic Growth Stimulator Inhibits the Growth and Respiration of Helicobacter pylori

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