Production of extracellular enzymes by two Pleurotus species using banana pseudostem biomass

Abstract

INDIA SummaryThe degradation of lignocellulosic biomass of banana pseudostem was investigated during solid state fermentation (SSF) by P. ostreutus and P.sujor-cuju. Both organisms proved to be efficient degraders of banana pseudostem biomass. P.ostreutus degraded hemicellulose (40% of dry weight., d.w.) better than cellulose (17.5% of d.w.) and lignin (10% 0f.d.w.). P. sujor-cuju also degraded hemicellulose (31% of d.w.) better than cellulose (12.4% of d.w.) and lignin (6% of d.w.). In both cases, a preferential removal of hemicellulose during the initial growth period and a delayed degradation of lignin were observed. The kinetics of cellulolytic, hemicellulolytic and lignolytic enzyme production in liquid culture were also examined. The activities of CMCase andJ-glucosidase were highest at 16 days of growth and avicelase activity was at its maximum after 24 days (CMCase -1.1 IU/ml. J-glucosidase -0.1 IU/ml and avicelase -0.09 IU/ml in the case of P.ostreutus; CMCase -1.0 IU/ml. J-glucosidase -0.09 IU/ml and avicelase 0.087 -IUlml in the case of P.sujor-cuju.).Xylanase and laccase activity reached their maximum after day 16 and day 24 of incubation, respectively. (Xylanase -1.1 IU/ml and laccase 3.0 IU/ml in the case of P. ostreutus; xylanase -1.0 IU/ml and laccase -3.6 IU/rnl in the case of P. sujor-cuju.). The efficient degrading capacity of the test fungi demonstrated their potential use in the conversion of banana pseudostem biomass into mycelial protein-rich fermented animal feed.

Development of an alternative technology for the oyster mushroom production using liquid inoculum

“…These values are in accordance with the literature (STURION, 1994;GHOSH et al, 1998). It can be seen that the substrate presents a low level of digestibility (15.40%).…”

Evaluation of Lignocellulosic Wastes for Production of Edible Mushrooms

“…This indicates that both species of mushroom were more effective in degrading cellulosic content of paddy straw and sorghum stalk rather than banana pseudostem. The degradation of lignocellulosic biomass of banana pseudostem by P.ostrreatus and P. sajor-caju [30] indicated that both organisms degraded hemicellulose better than cellulose.…”

Production of Enzymes and Biotransformation of Orange Waste by Oyster Mushroom, <i>Pleurotus pulmonarius</i> (Fr.) Quél.

“…Makkar et al [33] reported that the β-glucosidase enzyme is part of an enzymatic complex in Lentinus edodes, which also comprises MnP and laccase, where the hydrolase actively participates in the carbohydrates mobilization. Lower activities of this enzyme were produced by P. ostreatus and P. sajor-caju cultivated in banana waste: 0.090 U/mL and 0.087 U/mL, respectively [34].…”