Production of fertile transgenic <i>Brassica napus</i> by <i>Agrobacterium‐mediated</i> transformation of protoplasts

Wang, Y. P.; Sonntag, K.; Rudloff, E.; Han, Jeung-Sul

doi:10.1111/j.1439-0523.2004.01015.x

Cited by 24 publications

(11 citation statements)

References 22 publications

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“…The napin promoter from B. napus has proven to be very effective for seed-specific expression in different Brassica species (e.g., Wahlroos et al 2004;Wang et al 2005;Wu et al 2005). However, it would be advantageous to have additional promoters available for the coordinated, seed-specific expression of multiple genes.…”

Section: Introductionmentioning

confidence: 99%

Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters

Cheng

Wu²,

Vrinten³

et al. 2009

Transgenic Res

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Eicosapentaenoic acid (EPA, 20:5n-3) plays an important role in many aspects of human health. In our efforts towards producing high levels of EPA in plants, we investigated the effects of different host species, genes and promoters on EPA biosynthesis. Zero-erucic acid Brassica carinata appeared to be an outstanding host species for EPA production, with EPA levels in transgenic seed of this line reaching up to 25%. Two novel genes, an 18-carbon omega3 desaturase (CpDesX) from Claviceps purpurea and a 20-carbon omega3 desaturase (Pir-omega3) from Pythium irregulare, proved to be very effective in increasing EPA levels in high-erucic acid B. carinata. The conlinin1 promoter from flax functioned reasonably well in B. carinata, and can serve as an alternative to the napin promoter from B. napus. In summary, the judicious selection of host species and promoters, together with the inclusion of genes that enhance the basic very long chain polyunsaturated fatty acid biosynthetic pathway, can greatly influence the production of EPA in plants.

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Section: Introductionmentioning

confidence: 99%

Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters

Cheng

Wu²,

Vrinten³

et al. 2009

Transgenic Res

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“…The oil quality of B. napus depends on the composition, chain length, degree of desaturation, and functional groups of fatty acids (Friedt and Lühs 1998). In order to make the oil suitable for industrial purposes, some attempts have been made to modify the fatty acid composition of rapeseed using modern biotechnology, such as somatic hybridization (Fahleson et al 1994;Earle 1995, 1997;Wang et al 2003) and genetic transformation (Voelker et al 1992;Weier et al 1998;Schröder-Pontoppidan et al 2000;Taylor et al 2001;Wang et al 2004a).…”

mentioning

confidence: 99%

Intergeneric Somatic Hybridization Between Brassica napus L. and Sinapis alba L.

Wang

Sonntag

Rudloff

et al. 2005

Journal of Integrative Plant Biology

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Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L. Seven hybrids were obtained and verified by the simple sequence repeat and cleaved amplified polymorphic sequence analysis of the gene fae1, indicating that the characteristic bands from S. alba were present in the hybrids. The hybridity was also confirmed by chromosome number counting because the hybrids possessed 62 chromosomes, corresponding to the sum of fusion-parent chromosomes. Chromosome pairing at meiosis was predominantly normal, which led to high pollen fertility, ranging from 66% to 77%. All hybrids were grown to full maturity and could be fertilized and set seed after self-pollination or back-crosses with B. napus. The morphology of the hybrids resembled characteristics from both parental species. An analysis of the fatty acid composition in the seeds of F 1 plants was conducted and the seeds were found to contain different amounts of erucic acid, ranging from 11.0% to 52.1%.

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“…The probe derived from the OsNAS1 gene was labeled with digoxigenin, and hybridized according to the procedures of a previous study [11].…”

Section: Molecular Identification Of Transformed Plants (T 0 and T 1 )mentioning

confidence: 99%

Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress

Kong

Mao

et al. 2011

Chin. Sci. Bull.

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Salt stress is one of the major abiotic stresses in agricultural plants worldwide . We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na 2 CO 3 . Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis (2-DE) technology system. Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B. napus. These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to obtain the peptide mass fingerprints. Protein database searching revealed that 5 of these proteins are involved in salt tolerance: dehydrogenase, glutathione S-transferase, peroxidase, 20S proteasome beta subunit, and ribulose-1,5-bisphosphate carboxylase/oxygenase. The potential functions of these identified proteins in substance and energy metabolism, stress tolerance, protein degradation, and cell defense are discussed. The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa (cv. IAPAR 9). Brassica napus, nicotianamine synthase (NAS), salt-stress, differential proteins Citation:Kong F, Mao S J, Du K, et al. Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress.

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Production of fertile transgenic Brassica napus by Agrobacterium‐mediated transformation of protoplasts

Cited by 24 publications

References 22 publications

Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters

Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters

Intergeneric Somatic Hybridization Between Brassica napus L. and Sinapis alba L.

Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress

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