Production of Flavonoids in Cell Cultures of<i>Crataegus monogyna</i>

Kartnig, Theodor; Kögl, Gunter; Heydel, B.

doi:10.1055/s-2006-959756

Cited by 21 publications

(18 citation statements)

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“…the quantitative production of some secondary metabolites according to the time of cultivation. This finding corroborates with the observations deduced by other authors on the influence of some plant growth factors on the production of some metabolites in callus cultures of some medicinal plant parts (Kartnig et al, 1993;Bahorun et al, 1994;Saker and El Ashal, 1995;El-Bahr et al, 1997). Anthocyanins, anthraquinones and saponins were not detected in any extract and fraction (Table 2).…”

Section: Resultssupporting

confidence: 93%

In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae): part 2

Cimanga

Tona

Luyindula³

et al. 2004

Journal of Ethnopharmacology

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Section: Resultssupporting

confidence: 93%

In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae): part 2

Cimanga

Tona

Luyindula³

et al. 2004

Journal of Ethnopharmacology

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“…Despite the relative changes in the qualitative and quantitative patterns of polyphenolic biosynthesis, calli always produce appreciable amounts of phenolic compounds with maximal yields being intrinsically related to the optimum growth stages. In comparison to literature data on C. monogyna in vitro cultures [34,35], our cultures remain one of the highest polyphenol producing cell lines on an important time scale.…”

Section: Discussionmentioning

confidence: 65%

Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts

Bahorun¹,

Aumjaud

Ramphul

et al. 2003

Nahrung

115

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Crataegus (Hawthorn) has long been used as a folk medicine and is widely utilized in pharmaceutical preparations mainly because of its neuro- and cardiosedative actions and its low toxicity. The pharmacological effects of Crataegus have mainly been attributed to the polyphenolic contents. In this study, the production of polyphenols by ten-year-old Crataegus monogyna calli was studied in relation to growth variation and antioxidant capacity within a subculture period. Assays based on the Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and stability in oil-in-water emulsion were used to characterize the antioxidant actions of the callus cultures. High TEAC (3.66 micromol/g dry weight) and FRAP (208.19 micromol Fe2+/g dry weight) values were observed when maximal growth was reached(days 30-35), and this seemed to be influenced by optimum total phenol (47.40 mg/g dry weight), proanthocyanidin (20.81 mg/g dry weight), flavonoid (7.01 mg/g dry weight), anthocyanin (6.18 mg/g dry weight), (-)-epicatechin (1.77 mgl/g dry weight), procyanidin B2 (3.97 mg/g dry weight), and chlorogenic acid (1.11 mg/g dry weight) production during that period. The TEAC values were strongly associated with total flavonoids and to a lesser extent with total phenols, anthocyanins and total proanthocyanidins. The FRAP antioxidant values correlated to total phenols, proanthocyanidins and flavonoids, respectively. The polyphenolic rich calli were as effective as butylated hydroxytoluene (BHT) in preventing hydroperoxide and conjugated diene formation in a 30% oil-in-water emulsion prepared with stripped sunflower oil, during 7days storage at 30 degrees C. Crataegus monogyna cell culture represents an important alternative source for natural antioxidants.

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“…The developed method was applied to a commercial Crataegus extract, which is used in phytomedicine to treat cardiovascular problems. Crataegus was chosen because it is known to be rich in flavonoids and has been intensively studied in the past because of its interesting medicinal effects 7–10. This application was included with the aim of demonstrating that the conditions optimized for the mixture of the three selected flavonoids also hold for more complex mixtures of plant origin.…”

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confidence: 99%

Optimization of a liquid chromatography method based on simultaneous electrospray ionization mass spectrometric and ultraviolet photodiode array detection for analysis of flavonoid glycosides

Cuyckens¹,

Claeys²

2002

Rapid Comm Mass Spectrometry

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Different reversed-phase liquid chromatography (LC) columns of conventional dimensions were coupled to an ultraviolet photodiode array detector (UV-DAD) and a magnetic sector-type spectrometer, equipped with an electrospray ionization (ESI) source, by a laboratory-made flow splitter. A mixture of three flavonoid-O-glycosides was employed to examine the effects of the solvent composition, the flow rate, the stationary phase, the pH and the organic acid added, on the chromatographic separation, the UV-DAD detection, the ESI process and the entire LC system with ESI-MS and UV-DAD detection. In the positive ion mode, methanol containing 1% acetic acid was by far the most sensitive in ESI-MS analysis, whereas an acetonitrile/water mobile phase containing 0.5% formic acid was proved to give the best sensitivity in LC/ESI-MS/UV-DAD analysis. In the negative ion mode, the highest sensitivity was obtained with a mobile phase containing 0.1% formic acid, while addition of bases decreased the sensititvity. The optimal flow rate was higher in negative ESI (20-50 micro L/min) than in positive ESI (5 micro L/min), and the percentage of organic phase had an influence on the sensitivity of ESI-MS detection. With regard to the selection of a suitable C(18) reversed-phase LC column, a column which is well end-capped is to be preferred, because residual silanol groups appear to impair the separation of flavonoid glycosides. The optimized LC/ESI-MS/UV-DAD method was applied to a commercial Crataegus extract, which is used in phytomedicine to treat cardiovascular problems and is known to be rich in flavonoids. It is demonstrated how UV spectra and first-order ESI mass spectra allow a fast characterization of flavonoids, even if reference compounds are not available or at hand.

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Production of Flavonoids in Cell Cultures ofCrataegus monogyna

Cited by 21 publications

References 0 publications

In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae): part 2

In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae): part 2

Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts

Optimization of a liquid chromatography method based on simultaneous electrospray ionization mass spectrometric and ultraviolet photodiode array detection for analysis of flavonoid glycosides

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