Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture

Chaturvedi, Rakhi; Razdan, M. K.; Bhojwani, Sant S.

doi:10.1007/s00299-002-0565-6

Cited by 47 publications

(17 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the uninucleate stage, the wall of microspores is thin and it is easy to divert their mode of development from gametophytic to sporophytic. Androgenesis in Neem was earlier reported by Chaturvedi et al 7 from a tree growing at Delhi (North), India. However, as heterozygosity due to cross pollination and also the environmental conditions of the two regions have great influence on the physiology of parent plant, 8,9 consequently, nutritional requirements of the two plants may vary under the in vitro conditions.…”

Section: Resultssupporting

confidence: 61%

Increased production of azadirachtin from an improved method of androgenic cultures of a medicinal treeAzadirachta indicaA. Juss.

Srivastava

Chaturvedi²

2011

Plant Signaling & Behavior

View full text Add to dashboard Cite

Present report is the first direct evidence of azadirachtin production in androgenic haploid cultures of Azadirachta indica, a woody medicinal tree. Anther cultures at early-late-uninucleate stage of microspores were established on MS medium with BAP (5 μM), 2,4-D (1 μM) and NAA (1 μM) containing 12% sucrose. The calli, induced, were further multiplied on 2,4-D and Kinetin media. Shoots, differentiated on BAP (2.2 μM) + NAA (0.05 μM) medium, were elongated on MS + BAP (0.5 μM) and multiplied on MS + BAP (1 μM) + CH (250 mg/l). Thereafter, the shoots were rooted on ¼ MS + IBA (0.5 μM). Cytological analysis of the calli and regenerants have confirmed their haploid status with the chromosome number as 2n = x = 12. The haploid cell lines and leaves from in vitro grown plantlets were analyzed for azadirachtin by RP-HPLC and mass spectroscopy. Maximum azadirachtin (728.41 μg/g DW) was detected in calli supporting best shoot proliferation while least (49 μg/g DW) was observed in an undifferentiated line from maintenance medium. This study has brought us a step closer to develop genetically pure lines that could serve as new and attractive alternative ways of homogeneous controlled production of high value compounds, round the year, independent of geographical and climatic barrier.

show abstract

Section: Resultssupporting

confidence: 61%

Increased production of azadirachtin from an improved method of androgenic cultures of a medicinal treeAzadirachta indicaA. Juss.

Srivastava

Chaturvedi²

2011

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…Sanyal et al, (1981) also reported that 5% sucrose gave the best callus initiation and growth when neem stem sections were inoculated on MS basal medium supplemented with different combinations of IAA (0.1-0.5 mg/l), BAP (0.1-0.5 mg/l) and sucrose (5%). Chaturvedi et al, (2003) obtained the best callus proliferation response from anthers on MS medium supplemented with 9% sucrose, 1.0 µM 2, 4-D, 1.0 µM NAA and 5.0 µM BAP. The percentage of anthers callused and amount of callusing were markedly better than 3.0% or 12.0% sucrose.…”

Section: Discussionmentioning

confidence: 97%

Regeneration of Neem Plantlet by in Vitro Clonal Micropropagation

Bhandare¹

2019

IJRASET

View full text Add to dashboard Cite

The present study was carried out during 2014-15 with Neem (Azadirachta indica) is an evergreen tropical forest tree that is a renewable source of various useful products, to investigate optimal concentrations and combinations of plant growth regulators in the medium for efficient micropropagation. The present observation revealed that the highest callusing response 22.3±0.92% was observed in MS medium supplemented with 0.3 mg/l 2, 4-D; 0.3 mg/l Kn and 0.3 mg/l NAA in leaf explant and in seed explant it is 42.50.97% was observed in MS medium supplemented with 0.5 mg/l of each 2, 4-D and BAP. The highest shoot regenerating capacity (94%) was recorded in MS medium supplemented with 1.0+0.5 mg/l (BAP+NAA). Best rooting (95%) was observed in ½ strength of MS medium supplemented with 3 mg/l NAA and the highest number of average roots per micro shoots and average length ofroot were 15 and 4.9 cm respectively. Acclimatization and hardening was successful with survival rate of 87.5%.

show abstract

“…Chaicharoen et al (1996) observed maximum 9 shoot from callus, when medium was supplemented with 1.0 mg/l BA in Melia azedarach. Chaturvedi et al (2003) revealed that 5 µM BAP alone favored shoot induction from anther derived callus on MS medium. Srivastava et al (2009) observed maximum (78%) shoot regeneration when callus was sub cultured on MS medium containing 5 µM BAP alone in A. indica.…”

Section: Discussionmentioning

confidence: 99%

An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

Shah¹,

Wani²,

Ram³

et al. 2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Melia dubia Cav. (Meliaceae) is a multipurpose tree of tropical and subtropical regions mainly cultivated for its medicinal and industrial importance. Due to its versatile properties, it has been depleted in its natural environment. Moreover due to sluggish and poor seed germination, there is a threat of its gene pool exclusion from the natural habitat. The alternative method for conservation and efficient mass propagation is thus need of the hour. As per the extensive literature survey there is no report on efficient protocol for mass propagation of M. dubia through callus organogenesis. Therefore, the present work was aimed to develop in vitro organogenesis protocol for rapid and large scale production of planting material. From our results, maximum callus percentage, callus weight and fragile callus was observed on 1.0 mg/l benzylaminopurine (BAP) in combination with 0.5 mg/l naphthalene acetic acid (NAA). The callus differentiation was achieved at different concentrations of BAP and indole acetic acid (IAA). Multiple Shoot number per callus propagule 5.30 was observed on 0.5 mg/l BAP and 1 mg/l IAA concentration. The maximum rooting percentage (78.5%), root number per explant (4.33) and root length per explant (4.41 cm) was observed at 0.5 mg/l indol butyric acid (IBA) after 30 days of inoculation. Further the total flavonoid content, phenolic content and antioxidant properties of leaves of in-vitro regenerated plants where studied. Total flavonoids and phenolic content in leaves of in vitro Melia dubia was 0.56 ± 0.8 mg quercitin equivalent (QE) and 2.97 ± 0.17 mg gallic acid equivalent (GAE) respectively. The antioxidant property was further assed through measurement of DPPH radical scavenging activity. The in-vitro regeneration protocol can be exploited for commercial cultivation and fulfilling the growing demand for fresh explant material through mass propagation of M. dubia an economically important plant species.

show abstract

Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture

Cited by 47 publications

References 8 publications

Increased production of azadirachtin from an improved method of androgenic cultures of a medicinal treeAzadirachta indicaA. Juss.

Increased production of azadirachtin from an improved method of androgenic cultures of a medicinal treeAzadirachta indicaA. Juss.

Regeneration of Neem Plantlet by in Vitro Clonal Micropropagation

An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

Contact Info

Product

Resources

About