Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides

Yanagisawa, Mieko; Nakamura, Kanami; Ariga, Osamu; Nakasaki, Kiyohiko

doi:10.1016/j.procbio.2011.08.001

Cited by 162 publications

(96 citation statements)

References 22 publications

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“…The S. cerevisiae was aseptically inoculated on a petri dish using potato dextrose agar (PDA) medium with an ose and then incubated for 2 days at 30 °C. Preparation of inoculum (starter) was conducted in yeast malt peptone glucose (YMPG) liquid medium [3]. Sterile YMPG (10 mL) was added with ± 2 ose of S. cerevisiae inoculant from the PDA and then incubated for 24 hours at 30°C.…”

Section: Methodsmentioning

confidence: 99%

“…For example, lignocellulose is an alternative potential raw material for biofuel production but its high level of lignin makes it hard to degrade the lignocellulose [1][2][3][4]. Macroalgae are another potential biomass source and have various advantages: 1) do not compete with food sources, 2) have a high level of sugar, 3) have a low level of lignin, 4) have high productivity [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa

Kawaroe

Sari

Hwangbo

et al. 2015

J. Eng. Technol. Sci.

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Abstract. Red macroalgae have the potential to be processed into bioethanol due to their high carbohydrate and low lignin content. Gelidium latifolium and Gracilaria verrucosa are red macroalgae commonly found in Indonesian seas. Sometimes an over-supply of red macroalgae is rejected by the food industry, which opens up opportunities for others uses, e.g. for producing bioethanol. The objectives of this research were to analyze the influence of sulfuric acid concentration on hydrolysis of G. latifolium and G. verrucosa and to calculate the optimum fermentation process to produce bioethanol. G. latifolium and G. verrucosa were hydrolyzed using H 2 SO 4 at concentrations of 1%, 2%, 3%, and 4%, at a temperature of 121 °C and a pressure of 1.5 bar for 45 minutes. The process of fermentation was done using Saccharomyces cerevisiae in anaerobic conditions for 4, 5, 6 and 7 days. The results show that the optimum H 2 SO 4 concentrations to hydrolyze G. latifolium and G. verrucosa were 1% and 2% respectively. The number of S. cerevisiae cells in hydrolysate G. latifolium and G. verrucosa increased in the third adaptation. S. cerevisiae can convert sugar from G. latifolium and G. verrucosa into bioethanol through fermentation. The highest bioethanol yields were achieved on days five and six. Therefore, red macroalgae can be seen as a potential raw material for bioethanol production.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa

Kawaroe

Sari

Hwangbo

et al. 2015

J. Eng. Technol. Sci.

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“…These compounds include the predominantly β-1,3 glucose polymer laminarin (Nelson and Lewis 1974) and the alcohol sugar mannitol (Horn 2000), both of which can be readily hydrolysed and converted by microbes into a number of products including biofuels and platform chemicals (Suganya et al 2016). One such a highly researched conversion product, bioethanol (Horn et al 2000a, b;Adams et al 2009;Yanagisawa et al 2011), will be used in this study to assess overall process improvement.…”

Section: Introductionmentioning

confidence: 99%

“…Manns et al (2016) identifies ultracentrifugal milling which produces reproducible <0.5-mm-diameter particles (Yanagisawa et al 2011) as being too energy-consuming for large-scale seaweed biorefining and instead focussed on the effect of a less energy-intensive wet milling process for glucose release from L. digitata following enzymic saccharification. They found that due to the thin structure of the macroalgal blade, the seaweed was cut in a different manner to lignocellulosic material such as straw.…”

Section: Introductionmentioning

confidence: 99%

The effect of mechanical pre-processing and different drying methodologies on bioethanol production using the brown macroalga Laminaria digitata (Hudson) JV Lamouroux

Adams

Bleathman²,

Thomas

et al. 2017

J Appl Phycol

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Macroalgae are capable of generating more organic carbon per hectare than terrestrial plants without requiring land, fertiliser or fresh water to grow. In addition, they avoid the food versus fuel argument as they are not a major food source in Europe. In spite of these benefits, macroalgae are not yet fully exploited as a biomass source for bioenergy or platform chemical production in Europe, with one issue being the high harvesting and processing costs. This paper considers the impact of mechanical pre-processing of Laminaria digitata combined with different drying techniques and the effect of these on downstream processing to bioethanol. Results show that mechanically screw pressing macroalgae does enhance conversion to ethanol, but only when the material contains low levels of storage carbohydrates. This occurs in freezedried and air-dried samples. The addition of a press aid in the mechanical pre-processing step increases ethanol yields per gramme macroalgae, but due to the presence of the unutilised press aid in the fermentation, ethanol yields were lower overall. The two main findings from this work were (1) simple mechanical processing of L. digitata provides homogenisation and pumpability of macroalgae without negatively affecting subsequent microbial conversion to ethanol. (2) At higher carbohydrate concentrations, screw pressing confers no advantage in ethanol yields over strips of unprocessed kelp, making strips the more viable conversion option for low-input, large-scale processing.

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“…Consequently, in the past few years, studies were initiated on the feasibility of using marine biomass which is considered as the third generation biomass (Nigam and Singh, 2010) for ethanol production. The advantages of macroalgal biomass in comparison with terrestrial lignocellulosic sources are freedom from dependence on agricultural resources (Adams et al 2009), lignin (source of fermentation inhibitors) is either absent or in low quantity (Yanagisawa et al 2011), absorption of more carbon dioxide, high productivity per unit area and the possibility to utilize various water sources (Sahoo et al 2012). …”

Section: Introductionmentioning

confidence: 99%

Ethanol Production from the Biomass of Two Marine Algae, Padina tetrastromatica and Sargassum vulgare

Durbha¹,

Santosh²,

Guntuku³

et al. 2016

AJBB

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Macroalgae were identified as third generation carbon source for bioethanol production and possess several advantages over terrestrial biomass. Hence, two common seaweeds, P. tetrastromatica and S. vulgare have been evaluated as substrates for ethanol production. They were collected from coast of the Bay of Bengal at Visakhapatnam, Andhra Pradesh, India. Samples were cleaned, dried and crushed to powder which were subjected to separate treatments with dilute sulphuric acid, sodium hydroxide and a combination of both. Based on the quantities of sugars released from these treatments, pretreatment with 1% and 2% v/v sulphuric acid for 45 min at 121˚C were chosen for P. tetrastromatica and S. vulgare respectively and the quantities of sugars released were 0.32 and 0.44 g/g. The resulting hydrolyzates were detoxified by sequential treatment with ethyl acetate and calcium oxide and were then fermented employing Saccharomyces cerevisiae strain R3DSC5 which was deposited at IMTECH, India, with accession number MTCC 12377. Ethanol yield obtained with P. tetrastromatica (0.66 g/g) during the present work is more than the highest yield (0.45 g/g) reported so far for any marine algal biomass while that obtained with S. vulgare (0.38 g/g) is on par with the highest yield (0.386 g/g) reported for other species of Sargassum. This study shows that both the seaweeds are potential sources for bioethanol production and of the two, P. tetrastromatica is better.

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Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides

Cited by 162 publications

References 22 publications

Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa

Optimum Fermentation Process for Red Macroalgae Gelidium latifolium and Gracillaria verrucosa

The effect of mechanical pre-processing and different drying methodologies on bioethanol production using the brown macroalga Laminaria digitata (Hudson) JV Lamouroux

Ethanol Production from the Biomass of Two Marine Algae, Padina tetrastromatica and Sargassum vulgare

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