Production of immunogenic VP6 protein of bovine group A rotavirus in transgenic potato plants

Abstract

We report here the production of transgenic potato plants expressing the major capsid protein VP6 of bovine group A rotavirus (GAR). Transgenic plants under the control of a cauliflower mosaic virus 35S promoter, or a modified promoter linked to the tobacco mosaic virus 5'-untranslated sequence were positive for GAR antigens by ELISA. The expressed protein was consistent in size with VP6 of GAR by Western blot assay. The presence of the VP6 gene and its transcript was detected by PCR and RT-PCR. Adult BALB/c mice were immunized intraperitoneally with concentrated transgenic potato extracts emulsified in Freund's adjuvant. Sera collected after immunization showed the anti-VP6 response in ELISA and Western blot assay. These results suggest that the immunogenic VP6 protein expressed in plants could be useful for the preparation of diagnostic reagents.

Production of Two Vaccinating Recombinant Rotavirus Proteins in the Milk of Transgenic Rabbits

“…Production of rotavirus antigens or proteins in plants has been achieved but in all cases the expression levels were low (Matsumura et al, 2002;Kim & Langridge, 2003Wu et al, 2003;Yu & Langridge, 2003;Wigdorovitz et al, 2004). On the other hand, the purification of Figure 5.…”

Plant-derived vaccines against diarrhoeal diseases

“…Hepatitis B surface and core antigens Tobacco; potato; cherry tomatillo; soybean; lettuce [70][71][72][73][74][75][76] Hepatitis E virus ORF2 Tomato [77] Norwalk virus capsid protein Potato [58,59] Rabies virus glycoprotein Tomatoes; spinach [78][79][80] Cytomegalovirus glycoprotein B Tobacco [81,82] Rinderpest virus haemagglutinin Tobacco [83] Rabbit haemorrhagic disease virus VP 60 protein Potato [84] Foot-and-mouth disease virus VP1 antigen Alfalfa; potato [85][86][87][88] Transmissible gastroenteritis coronavirus glycoprotein S Corn; potato [43,89,90] HIV p24 Tobacco [91] Measles virus haemagglutinin Carrot; tobacco [92][93][94][95] Human papillomavirus type 16 major capsid protein Potato; tobacco [96,97] VP6 protein of rotavirus Potato [98] Canine parvovirus peptide Arabidopsis [99] Respiratory syncytial virus F and G protein Tobacco; tomato; apple [100,101] Enterotoxigenic E. coli Potato; corn [32,[41][42][43]102] Enteropathogenic E. coli Tobacco [103] Vibrio cholerae toxin Tobacco; tomato; potato [48][49][50]…”

Production of vaccines for treatment of infectious diseases by transgenic plants

“…Due to their expensiveness making them unavailable to the majority of the population in the developing world and the recent emergence of several novel serotypes there is however a big demand for developing new and better rotavirus vaccines (Pera et al, 2015), an aim for which also transgenic plants have been utilized a lot during the recent years. Rotavirus capsid proteins have been expressed by various research groups: VP6 has been expressed in tomato (Chung et al, 2000;Chung et al, 2001), alfalfa (Dong et al, 2005), potato (Yu and Langridge, 2003), VP7 has been expressed in potato (Wu et al, 2003;Choi et al, 2005), VP8* in transplastomic tobacco plants (Lentz et al, 2011), bovine VP8* in potato (Matsumura et al, 2002) and N. benthamiana in recombinant form (Perez-Filgueira et al, 2004a). In addition, NSP4-CT-B fusion protein has been expressed in potato (Arakawa et al, 2001;Kim and Langridge, 2004), VP6-BSSV fusion protein in Chenopodium amaranticolor (Zhou et al, 2010) and VP2, VP6 and VP7 have been co-expressed in the form of VLPs in tobacco (Yang et al, 2011).…”