Production of interspecific somatic hybrid plants between Asparagus officinalis and A. macowanii through electrofusion

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

“…Production of somatic hybrids in genus Asparagus has only been reported in combination between A. officinalis and A. macowanii [4]. A. macowanii, a wild species used for ornamental purposes, A. macowanii was reported to be resistant to Phomopsis asparagi which is a causal fungus of a serious disease, stem blight of asparagus [2], it is expected that the resistant trait of this species to P. asbaragi could be …”

“…As disease-resistant germplasms, Asparagus densiflorus, an ornamental species, was reported to be resistant to F. oxysporum and F. moniliforme [1], and A. macowanii to Phomopsis asparagi [2]. However, sexual cross of A. officinalis with these resistant species have been unsuccessful, probably due to incompatibility barriers [3,4].…”

“…The embryogenic callus has efficiently been utilized as the source of protoplasts from which plant regeneration was achieved [3,6,7]. Furthermore, interspecific somatic hybrids and genetically manipulated plants have been produced using the protoplast culture system [4,8].…”

Review of forests, wood products and wood biotechnology of Iran and Germany - Part III

“…On the other hand, karyotypic data are of importance in showing the species interrelationships. Researches overcome sexual breeding barriers of plant species by somatic hybridization through protoplast fusion (Yamaguchi and Shiga, 1993, Kunitake et al 1996and Tsukatani et al 2002, but different genomic size and characteristics are the main obstacles for next generations. Although such complexity may allow the maintenance of large reserves of genetic variability within and among populations, favouring potential long-term breeding progress, but limiting genetic 154 gains in short-and long-term breeding programs (Casler et al 2000).…”

Features in Stem Blight Resistance Confirmed in Interspecific Hybrids of <i>Asparagus officinalis</i> L. and <i>Asparagus kiusianus</i> Makino

“…Interspecific hybrids, produced by cell fusion technique using electro pulsation from protoplasts of A. officinalis and A. macowanii, did not grow into adult plants, since they might have an abnormal genome composition (Kunitake et al, 1996). A. kiusianus Makino originating from the restricted Japan's coastal regions, along the Sea of Japan from Yamaguchi Prefecture to Northern Kyushu, is a dioecious species like A. officinalis (Makino, 1907) (Fig.…”