Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi

Bonugli-Santos, Rafaella Costa; Durrant, Lúcia Regina; Silva, Manuela da; Sette, Lara Durães

doi:10.1016/j.enzmictec.2009.07.014

Cited by 154 publications

(79 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results agree with those of previous reports (Jones 2000;Raghukumar 2008), which showed that marine fungi were known to produce functional enzymes at pH 7 to 8. The finding that enzymes of P. chrysogenum FU42 are active in extreme conditions is interesting because it indicates that the fungus can trigger and produce much more specific enzymes (Pointing and Hyde 2000;Bonugli-Santos et al 2010). These results indicate that enzymes from marinederived fungi are considerably different from those produced by their terrestrial counterparts because the enzymes retained their activities in extreme conditions where proteins can be denatured (Raghukumar 2008).…”

Section: Comparison Between Marine-derived and Terrestrial Fungimentioning

confidence: 92%

Halo-tolerance of Marine-derived Fungi and their Enzymatic Properties

Lee¹,

Lee²,

Heo³

et al. 2015

BioResources

View full text Add to dashboard Cite

Marine environments are unique habitats for microorganisms and represent uninvestigated areas that possess valuable resources. Fungi may also be important because they contribute to marine ecosystems as decomposers. The aims of this study were to investigate the halotolerance of marine-derived fungi and their enzymatic properties. Eighteen fungal strains representing 11 different species were used, which included 17 ascomycetes and a zygomycete. The majority were not affected by salinity and showed endo-glucanase (EG) and β-glucosidase (BGL) activities. Interestingly, the cellulolytic enzyme activity derived from Penicillium chrysogenum FU42 increased with salinity. To investigate whether this increase was due to an adaptation or an innate ability of the species, P. chrysogenum KCTC6933, which originated from a terrestrial environment, was used as a control, and its enzymatic properties were compared. Consequently, P. chrysogenum FU42, which was derived from the ocean, showed unique enzymatic properties that might enable the fungus to live in the ocean and contribute to the nutrient cycle in marine ecosystems.

show abstract

Section: Comparison Between Marine-derived and Terrestrial Fungimentioning

confidence: 92%

Halo-tolerance of Marine-derived Fungi and their Enzymatic Properties

Lee¹,

Lee²,

Heo³

et al. 2015

BioResources

View full text Add to dashboard Cite

show abstract

“…Thus, there is a greater production with an irradiated sample at 10,000 Gy. This change is probably due to changes in the chemical composition of the substance blocked in the metabolic pathways by radiation (Silva et al, 2010).…”

Section: Activity Of Laccase (Lac)mentioning

confidence: 99%

“…To quantify the manganese peroxidase (Bonugli-Santos et al, 2010), oxidation of phenol red (0.01% v / v) plus 500 μL of the enzyme extract, sodium lactate (0.25 M), bovine albumin (0.5% w / v) MnSO4 (2 mM) and H2O2 in citrate phosphate buffer (20 mM, pH 4.5) was measured. The reading was done at 610 nm.…”

Section: Activity Of Manganese Peroxidase (Mnp)mentioning

confidence: 99%

“…Laccase is an oxidase which catalyses the reduction of H 2 and O 2 to oxidize aromatic amines (D'Souza et al, 2006). As these enzymes do not have substrate specificity, they are employed in degradation, with application in chemical, food, agriculture, paper, textiles, cosmetics industries and in bioremediation treatments (Bonugli-Santos et al, 2010;Sette et al, 2008;Pearce, 1997). Many microorganisms have been investigated in relation to their ability to produce enzymes, among which filamentous fungi are of great biotechnological interest.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of gamma radiation on enzymatic production of lignolytic complex by filamentous fungi

Baptista¹,

Solidônio²,

Arruda³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

This work aimed to study the effect of gamma radiation on the production of enzymes by filamentous fungi present in the seawater used for thermoelectric Termope S / A, in the vicinity of Port of Suape, Pernambuco. The isolated microorganisms were screened for their ability to produce enzymes. Subsequently, the fungi were irradiated at 3 doses (500, 1000 and 1500 Gy), using 4 inoculation techniques: lyophilisation, scraping of the spores, glass beads and agarose block. A fermentation assay for enzyme production was made in simple batch using a bioreactor New Brunswick, model Bioflo 110. The best enzyme produced was lignin peroxidase (676 U/L) by Aspergillus awamori in 500 Gy dose. Manganese peroxidase enzyme (1720 U / L), produced by three species of fungi (Penicillium sp.), was the highest in 500 Gy dose. Aspergillus terreus produced laccase enzyme (980 U/L) better in 500 Gy dose. In the assay of the fermentation bioreactor, the best results were found in BH-amid corn steep liquor, where 650 U/L of laccase was produced at the baseline and also in the medium containing corn steep liquor, where 620 U/L laccase was produced during 72 h. This is better than Sabouraud. The use of gamma radiation increased the production of enzymes by filamentous fungi compared to tests without radiation. Statistical analysis shows there are no significant differences between the inoculation techniques, and the best dose of radiation tested was 500 Gy. In trial bioreactor containing Bushnell Haas + corn steep, Penicillium sp. produced larger amounts of phenoloxidases enzymes compared to Sabouraud medium. Irradiation in a dose of 500 Gy stimulates A. awamori, A. terreus and Penicillium sp. to produce phenoloxidases enzymes.

show abstract

“…Several laccases have been purified from the culture supernatant of C. cladosporioides, which usually showed relative activities over a range of pH 3 to 6 and 40 to 70°C [17,18]. Previous research of C. cladosporioides mostly focused on its good capacity for dye decolorization and various enzyme activities in mesophilic temperatures [7,19,20]. There was only one report about psychrophilic C. cladosporioides, which was isolated from Antarctica at either 4°C or 15°C with the ability to produce endoglucanase [21].…”

Section: Introductionmentioning

confidence: 99%

Synergy of crude enzyme cocktail from cold-adapted Cladosporium cladosporioides Ch2-2 with commercial xylanase achieving high sugars yield at low cost

Yang

Fan

et al. 2014

Biotechnol Biofuels

View full text Add to dashboard Cite

Background: The efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysaccharide hydrolases and the h ydrolysis of holocellulose. If we include in hydrolase mixture construction the ligninase involved in lignin degradation, which mainly includes laccase, manganese peroxidases (MnP) and lignin peroxidase (LiP), it is feasible that this could greatly improve the fermentable sugars yield. Results: The psychrophilic lignocellulosic enzymes system of Cladosporium cladosporioides Ch2-2 including ligninase and polysaccharide hydrolases was suitable for selective delignification and efficient saccharification of biomass with wide thermal adaptability. The purified laccase was optimally active at 15°C and pH 3.5, exhibiting high thermostability over a broad range of temperatures (between 4 and 40°C). In addition, manganese-independent peroxidase (MIP), a special type of ligninase with the capacity to oxidize dimethyl phthalate (DMP) in the absence of H 2 O 2 and Mn

show abstract

Production of laccase, manganese peroxidase and lignin peroxidase by Brazilian marine-derived fungi

Cited by 154 publications

References 33 publications

Halo-tolerance of Marine-derived Fungi and their Enzymatic Properties

Halo-tolerance of Marine-derived Fungi and their Enzymatic Properties

Effects of gamma radiation on enzymatic production of lignolytic complex by filamentous fungi

Synergy of crude enzyme cocktail from cold-adapted Cladosporium cladosporioides Ch2-2 with commercial xylanase achieving high sugars yield at low cost

Contact Info

Product

Resources

About