Production of Microalgal Biomass Using Aquaculture Wastewater as Growth Medium

Hawrot-Paw, Małgorzata; Koniuszy, Adam; Gałczyńska, Małgorzata; Zając, Grzegorz; Szyszlak-Bargłowicz, Joanna

doi:10.3390/w12010106

Cited by 80 publications

(31 citation statements)

References 35 publications

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“…The average optical density ranged from 0.140 ± 0.00 (AWW 40 ) to 0.201 ± 0.00 (AWW 60 ) (Figure 4B). Similar values of optical density (OD 0.304) were obtained by Hawrot-Paw et al [34] during the cultivation of C. minutissima in the same type of salted AWW. This may be a result of different nutritional requirements of microalgae [51,52].…”

Section: Optical Density Of Microalgal Culturessupporting

confidence: 84%

“…The average optical density ranged from 0.140 ± 0.00 (A (AWW60) (Figure 4B). Similar values of optical density (OD 0 Hawrot-Paw et al [34] during the cultivation of C. minutissima in AWW. This may be a result of different nutritional requiremen The increase in optical density with higher doses of AWW indica of nutrients in the medium promotes the growth and developmen also confirmed by Bhatnagar et al [53] when they cultivated m enriched medium.…”

Section: Optical Density Of Microalgal Culturessupporting

confidence: 52%

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Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Ratomski¹,

Hawrot-Paw²

2021

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Microalgal biomass and its cellular components are used as substrates for the production of fuels. A valuable group among the components of microalgal biomass is lipids, which act as a precursor for the production of biodiesel in the transesterification process. Some methods, including the creation of stressful conditions, are applied to increase the accumulation of lipids. This study aimed to determine the effect of limited nutrient access on the growth and development of the microalga Chlorella vulgaris and the amount of lipids stored in its cells. Aquaculture wastewater (AWW) was used in the study as a source of nutrients at doses of 20%, 40%, 60%, 80% and 100%. The amount of microalgal biomass, optical density, lipid content after extraction of the biomass in Soxhlet apparatus and chlorophyll a content were determined. It was observed that the microalgae efficiently used the nutrients contained in the AWW. The largest amount of biomass was obtained in AWW80 (727 ± 19.64 mg·L−1). The OD680 (0.492 ± 0.00) determined under the same conditions was almost five times higher in AWW than in the synthetic medium. Under nutrient-stress conditions, the content of lipids in biomass ranged from 5.75% (AWW80) to 11.81% (AWW20). The highest content of chlorophyll a in microalgal cells was obtained in AWW20 (206 ± 11.33 mg∙m−3).

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Section: Optical Density Of Microalgal Culturessupporting

confidence: 84%

Section: Optical Density Of Microalgal Culturessupporting

confidence: 52%

Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Ratomski¹,

Hawrot-Paw²

2021

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“…In that context, commercial fertilizers can be used as a nutrient source for cultivation and economically viable production of microalgae. Previous studies have mainly been focused on the culture of microalgae by using aquaculture wastewater (Ramanna et al 2014;Caporgno et al 2015;Hawrot-Paw et al 2020). In the current study, commercial fertilizer NPK (20:20:10) purchased from the local market at a very low price of 1 US $ for 1 kg was added in aquaculture wastewater (AWW) to determine if these additions could enhance the biomass production of C. vulgaris under laboratory condition.…”

Section: Introductionmentioning

confidence: 99%

Supplementing wastewater with NPK fertilizer as a cheap source of nutrients in cultivating live food (Chlorella vulgaris)

2021

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Introduction The decline in fishery resources from the wild has led to an ever increasing focus on aquaculture in recent years. With increasing aquaculture of animal species, there is an increasing need for suitable microalgae in the production of these animals. However, cultivation of microalgae in expensive pure chemical media is one of the major challenges facing large-scale cultivation of microalgae. Purpose The present study investigated the suitability of aquaculture wastewater (AWW) supplemented with NPK (nitrogen:phosphorus:potassium) fertilizer as a cheap source of nutrient to cultivate a microalga Chlorella vulgaris (C. vulgaris). Methods C. vulgaris with an initial cell density of 0.8 × 106 cells/mL was batch cultured in AWW supplemented with NPK at 0.1, 0.5, 1.0 g/L and BBM for 20 days under laboratory conditions using 2000 mL Erlenmeyer flasks. The proximate composition, chlorophyll, minerals, and vitamins analysis of C. vulgaris biomass were done using standard analytical methods. Results The highest values in optical density (4.872 ± 0.025), dry cell weight (2.858 ± 0.015 g/L), specific growth rate (0.2097 ± 0.0038 day–1), and biomass productivity (0.1701 ± 0.0007 g/L/day) were obtained in C. vulgaris grown in AWW + 1.0 NPK medium. The total chlorophyll, protein, lipid, and carbohydrate content of the microalgae biomass were in the range of 0.05–0.862%, 44.062–57.089%, 17.064–23.260%, and 15.217–21.896%, respectively. Furthermore, microalgae grown in AWW + 1.0 NPK showed good vitamin and mineral content compared to BBM grown alga. Conclusion These findings indicated that the AWW + 0.1 NPK, AWW + 0.5 NPK, and AWW + 1.0 NPK are potential growth media for C. vulgaris cultivation and can replace the BBM medium, which is very expensive and less accessible to users.

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“…While land is still needed for microalgae cultivation, this does not have to be the high-quality arable land required for agricultural activities. Microalgal production may utilize wastewater as a nutrient source and/or recycled CO 2 from industrial facilities or geothermal power plants (Sayre, 2010;Hawrot-Paw et al, 2020).…”

Section: Microalgaementioning

confidence: 99%

The Essentials of Marine Biotechnology

Barbier²,

et al. 2021

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Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better health and well-being, new biomedicines, natural cosmeceuticals, environmental conservation, and sustainable energy sources. These societal needs stimulated the interest of researchers on the diverse and underexplored marine environments as promising and sustainable sources of biomolecules and biomass, and they are addressed by the emerging field of marine (blue) biotechnology. Blue biotechnology provides opportunities for a wide range of initiatives of commercial interest for the pharmaceutical, biomedical, cosmetic, nutraceutical, food, feed, agricultural, and related industries. This article synthesizes the essence, opportunities, responsibilities, and challenges encountered in marine biotechnology and outlines the attainment and valorization of directly derived or bio-inspired products from marine organisms. First, the concept of bioeconomy is introduced. Then, the diversity of marine bioresources including an overview of the most prominent marine organisms and their potential for biotechnological uses are described. This is followed by introducing methodologies for exploration of these resources and the main use case scenarios in energy, food and feed, agronomy, bioremediation and climate change, cosmeceuticals, bio-inspired materials, healthcare, and well-being sectors. The key aspects in the fields of legislation and funding are provided, with the emphasis on the importance of communication and stakeholder engagement at all levels of biotechnology development. Finally, vital overarching concepts, such as the quadruple helix and Responsible Research and Innovation principle are highlighted as important to follow within the marine biotechnology field. The authors of this review are collaborating under the European Commission-funded Cooperation in Science and Technology (COST) Action Ocean4Biotech – European transdisciplinary networking platform for marine biotechnology and focus the study on the European state of affairs.

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Production of Microalgal Biomass Using Aquaculture Wastewater as Growth Medium

Cited by 80 publications

References 35 publications

Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Influence of Nutrient-Stress Conditions on Chlorella vulgaris Biomass Production and Lipid Content

Supplementing wastewater with NPK fertilizer as a cheap source of nutrients in cultivating live food (Chlorella vulgaris)

The Essentials of Marine Biotechnology

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