“…Two different age-groups, 0-1-year-old (these were basically 6-month to 1-year old plants) and 2-4-years-old, fresh Phyllostachys rubromarginata (red margin) bamboo plants were obtained from Lewis Bamboo Inc. All the enzymes and chemicals used in this research are the same as mentioned previously. 1 Sodium hydroxide (NaOH) pellets, sodium carbonate (Na 2 CO 3 ), sodium bicarbonate (NaHCO 3 ), hydrogen peroxide (30% H 2 O 2 ), and an acidic buffer of acetic acid and sodium acetate were American Chemical Society (ACS) grade from VWR. For processing, a Launderometer (SDL Atlas), bamboo splitters, milling machine, reactor, dryer, scanning electron microscope (SEM, FEI Quanta 200 3D) and energy-dispersive X-ray spectroscopic (EDX, LYRA3 TESCAN, Ametek Materials Analysis Division), and regular and/or microbalances were used as mentioned previously.…”