Production of Phenol Oxidases and Peroxidases by Wood-Rotting Fungi

Szklarz, Grazyna D.; Antibus, Robert K.; Sinsabaugh, Robert L.; Linkins, Arthur E.

doi:10.2307/3759705

Cited by 109 publications

(65 citation statements)

References 3 publications

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“…Peroxidase and laccase activities were determined by the oxidation of 0-dianisidine (∈460 = 29.400 M -1 cm -1 ) with and without the adding of hydrogen peroxide (16). The Manganese Peroxidase activity was determined by the phenol red oxidation (∈610 = 4.460 M -1 cm -1 ) (5).…”

Section: Enzymatic Assaysmentioning

confidence: 99%

Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian basidiomycetes fungi

Machado

Matheus

Bononi

2005

Braz. J. Microbiol.

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Remazol Brilliant Blue R (RBBR) dye was used as substrate to evaluate ligninolytic activity in 125 basidiomycetous fungi isolated from tropical ecosystems. The extracellular RBBR decolorizing activity produced when selected fungi were grown in solid media and in soil contaminated with organochlorines was also evaluated. A total of 106 fungi decolorized the RBBR during the growth in malt extract agar (MEA, 2%); 96 fungi showed a mycelia growth and decolorization activity stronger than the P. chrysosporium used as reference. Extracellular extracts of 35 selected fungi grown on solid medium with sugar cane bagasse (BGS) were evaluated for RBBR decolorization and peroxidase activity. All fungi showed peroxidase activities, but 5 of those were unable to decolorize the RBBR. Different patterns of ligninolytic enzymes were detected in 12 fungi extracts. Mn-dependent peroxidase (MnP) was produced by Peniophora cinerea, Psilocybe castanella, three strains of Trametes villosa, T. versicolor, Melanoporia nigra and Trichaptum byssogenum. All 12 fungi had laccase activity. Trogia buccinalis showed the highest RBBR decolorization and did not produce MnP activity. RBBR decolorization without MnP production was also observed for three strains of Lentinum tested. Higher levels of peroxidase and laccase cannot be related to high RBBR decolorization. RBBR decolorization by extracellular extract was also detected during the growth of P. castanella, L. crinitus, P. cinerea and two strains of T. Villosa in pentachlorophenol-and hexachlorobenzene-contaminated soils. These fungi showed higher RBBR decolorization when grown in the presence of organochlorine compounds than when in non contaminated soil.

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Section: Enzymatic Assaysmentioning

confidence: 99%

Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian basidiomycetes fungi

Machado

Matheus

Bononi

2005

Braz. J. Microbiol.

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“…A schematic representation of the strategies employed to distinguish between individual phenol-oxidizing enzymes by subtractive activity assays (Szklarz et al, 1989) and sequential enzymatic staining of gels using native-PAGE is shown in Fig. 1.…”

Section: Enzyme Assaymentioning

confidence: 99%

Enzymatic Staining for Detection of Phenol-Oxidizing Isozymes Involved in Lignin- Degradation by Lentinula edodes on Native-PAGE

Tanesaka¹,

Saeki²,

Kochi³

et al. 2012

Gel Electrophoresis - Advanced Techniques

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“…MnP determinations were based on the oxidation of phenol red, according to Kuwahara et al (19). Laccase was determined via the H2O2-independent oxidation of syringaldazine (ethanol solution) to its quinone form (21). The supernatants were obtained following centrifugation of the content of whole flasks at 17,000 rpm for 30 min at 5ºC.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons by soil fungi

Clemente¹,

Anazawa²,

Durrant³

2001

Braz. J. Microbiol.

100

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Thirteen deuteromycete ligninolytic fungal strains were grown in media containing polycyclic aromatic hydrocarbons (PAHs), for 6 and 10 days. The PAHs were added directly with the inocula or on the third day of cultivation. A selection of the best strains was carried out based on the levels of degradation of the PAHs and also on the ligninolytic activities produced by the fungi. The selected strains were cultivated for 3, 6, 9, 12 and 15 days in the PAHs-containing media. Degradation of PAHs, as measured by reversed-phase HPLC on a C18 column, varied with each strain as did the ligninolytic enzymes present in the culture supernatants. Highest degradation of naphthalene (69%) was produced by the strain 984, having Mn-peroxidase activity, followed by strain 870 (17%) showing lignin peroxidase and laccase activities. The greatest degradation of phenanthrene (12%) was observed with strain 870 containing Mn-peroxidase and laccase activities. When anthracene was used, the strain 710 produced a good level of degradation (65%).

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Production of Phenol Oxidases and Peroxidases by Wood-Rotting Fungi

Cited by 109 publications

References 3 publications

Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian basidiomycetes fungi

Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian basidiomycetes fungi

Enzymatic Staining for Detection of Phenol-Oxidizing Isozymes Involved in Lignin- Degradation by Lentinula edodes on Native-PAGE

Biodegradation of polycyclic aromatic hydrocarbons by soil fungi

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