Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA

Николаева, Т. Н.; Загоскина, Н. В.; Zaprometov, M. N.

doi:10.1134/s1021443709010075

Cited by 31 publications

(11 citation statements)

References 8 publications

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“…They found that addition of benzylaminopurine and kinetin to the culture media led to increasing the phenolic compound biosynthesis. In addition, NIKOLAEVA et al (2009) reported that growing transgenic tea callus tissue (strain IFR ChS-2) on nutrient medium containing NAA at concentration of 2 × 10 -5 M stimulated total soluble phenolics more than total phenolics which presented in intact plants.…”

Section: Resultsmentioning

confidence: 99%

Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

El-Baz

Mohamed

Ali

2021

Nova Biotechnol. Chim.

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Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.

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Section: Resultsmentioning

confidence: 99%

Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

El-Baz

Mohamed

Ali

2021

Nova Biotechnol. Chim.

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“…One of the biggest challenges in the in vitro production of secondary metabolites has been the efficiency of their production compared with the culture systems under field conditions. In this sense, studies are required to increase the concentration of these compounds in in vitro conditions [ 18 ], associated with a reduction in costs of micropropagation systems in order to make their production economically feasible under these conditions [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

2020

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The efficient production of plant-derived medicinal compounds (PDMCs) from in vitro plants requires improvements in knowledge about control of plant or organ development and factors affecting the biosynthesis pathway of specific PDMCs under in vitro conditions, leading to a realistic large-scale tool for in vitro secondary metabolite production. Thus, this study aimed to develop an in vitro technique, through the induction and proliferation of calli, for production of plant fresh weight, and to compare the PDMC profile obtained from the plants versus in vitro calli of Phyllanthus amarus. It was successfully possible to obtain and proliferate two types of calli, one with a beige color and a friable appearance, obtained in the dark using Murashige and Skoog (MS) medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and a second type with a green color, rigid consistency, and nonfriable appearance obtained under light conditions and MS medium plus 6-benzyladenine (6-BA). In vitro micropropagated plants that gave rise to calli were also acclimatized in a greenhouse and cultivated until obtaining the mass for PDMC analysis and used as a control. While the micropropagated-derived plants concentrated the lignans niranthin, nirtetralin, and phyllanthin, the Phyllanthus amarus calli proliferated in vitro concentrated a completely different biochemical profile and synthesis of compounds, such as betulone, squalene, stigmasterol, and β-sitosterol, in addition to others not identified by GC-MS database. These results demonstrate the possibility of applying the calli in vitro from Phyllanthus amarus for production of important PDMCs unlike those obtained in cultures of differentiated tissues from field plants.

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“…It has been reported that in actively growing calli of wheat and barley, high concentrations of 2,4-D suppressed the elongation growth but stimulated cell division. Recently, a similar concentration of 2,4-D was found to be optimum for callus cultures of C. sinensis producing phenolic compounds (Nikolaeva et al 2009). Thus a medium range concentration optimization of 2,4-D obtained for Camellia cells supports the previously reported results.…”

Section: Effect Of Different Media Auxin Concentration and Cytokininmentioning

confidence: 99%

Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

Grover

Yadav

Biswas

et al. 2011

Plant Cell Tiss Organ Cult

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Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS or Heller medium containing different levels of 6-benzyladenine (BA) (0.05-2 mg l -1 ), 2,4-dichlorophenoxyacetic acid (2,4-D) (1-10 mg l -1 ), and sucrose (10-50 g l -1 ). Moreover, the pH of the medium was varied from 5.2-6.2. In addition, cultures were subjected to light irradiation as well as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major monoterpenoids obtained were a-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol, benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds were obtained from cell suspension cultures grown in MS medium containing 5 mg l -1 2,4-D, 1 mg l -1 BA and 30 g l -1 sucrose at pH of 5.8 with incubation in complete darkness.

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Production of phenolic compounds in callus cultures of tea plant under the effect of 2,4-D and NAA

Cited by 31 publications

References 8 publications

Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

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