Production of l-Methionine from 3-Methylthiopropionaldehyde and O-Acetylhomoserine by Catalysis of the Yeast O-Acetylhomoserine Sulfhydrylase

Abstract

L-Methionine is an essential bioactive amino acid with high commercial value for diverse applications. Sustained attentions have been paid to efficient and economical preparation of L-methionine. In this work, a novel method for L-methionine production was established using O-acetyl-homoserine (OAH) and 3-methylthiopropionaldehyde (MMP) as substrates by catalysis of the yeast OAH sulfhydrylase MET17. The OAH sulfhydrylase gene Met17 was cloned from Saccharomyces cerevisiae S288c and overexpressed in Escherichia coli BL21. A 49 kDa MET17 was detected in the supernatant of the recombinant E. coli strain BL21-Met17 lysate with IPTG induction, which exhibited the biological activity of L-methionine biosynthesis from OAH and MMP. The recombinant MET17 was then purified from E. coli BL21-Met17 and used for in vitro biosynthesis of L-methionine. The maximal conversion rate (86%) of OAH to L-methionine catalyzed by purified MET17 was achieved by optimization of the molar ratio of OAH to MMP. The method proposed in this study provides a possible novel route for the industrial production of L-methionine.

Engineering O‐Succinyl‐L‐Homoserine Mercaptotransferase for Efficient L‐Methionine Biosynthesis by Fermentation‐Enzymatic Coupling Route

“…However, it requires harsh reaction conditions and toxic reagents, resulting in environmental unfriendliness, which is a big challenge for the development of advanced industries. [11] Microbial fermentation is regarded as the potential strategy with cheap raw materials and clean process, but the metabolic synthesis pathway of L-methionine in microorganisms is complex, requiring supply of sulfur source and one-carbon unit. Moreover, it is affected by multi-level regulation, which is difficult to realize the industrial production.…”

Effect of Methionine on Gene Expression in Komagataella phaffii Cells

“…Methionine and its derivatives implement various cell functions, such as DNA methylation, lipid homeostasis, and polyamine synthesis [28,29]. In yeast and mammalian cells, S-adenosylmethionine (a methionine derivative) has been shown to directly activate the target of rapamycin (TOR) complex I [30].…”

O-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum

“…When L-homoserine is used as the precursor, it needs to be activated by HCl before reacting with methanethiol to produce L-methionine [ 5 ]. Whereas, when OAH is selected as the precursor, it can directly react with methanethiol or 3-methylthiopropionaldehyde to form L-methionine [ 6 ]. Therefore, the production of OAH is very important for the industrial production of L-methionine.…”