2000
DOI: 10.1007/s11627-000-0007-5
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Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency

Abstract: Here we present a routine and efficient protocol for year-round production of fertile transgenic maize plants. Type II callus derived from maize Hi II immature zygotic embryos was transformed using the PDS 1000/He biolistic gun and selected on bialaphos. In an effort to improve the transformation protocol, the effects of gold particle size and callus morphology on transformation efficiency were investigated. Reducing gold particle size from 1.0 mm or 0.6 mm resulted in a significant increase in the rate of rec… Show more

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Cited by 164 publications
(141 citation statements)
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“…We used a particle bombardment protocol (Frame et al 2000) in which a plasmid containing a selectable marker, bar, is co-bombarded with a plasmid containing the wheat glutenin gene. The glutenin expression plasmid contains a wheat genomic DNA fragment including the 1Dx5 coding sequence and flanking sequences (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…We used a particle bombardment protocol (Frame et al 2000) in which a plasmid containing a selectable marker, bar, is co-bombarded with a plasmid containing the wheat glutenin gene. The glutenin expression plasmid contains a wheat genomic DNA fragment including the 1Dx5 coding sequence and flanking sequences (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Embryogenic callus derived from the genotype Hi-II was used as a substrate for bombardment. Herbicide resistance and presence of the Glu-1Dx5 gene were used as the criteria to select callus for regeneration to T 0 plants (Frame et al 2000). The T 0 plants from 12 independent events were crossed with B73 to produce 54 ears containing F 1 kernels.…”
Section: Resultsmentioning
confidence: 99%
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“…The promoter sequence in P2P46 construct was from c-zein (black). The coding sequence (CDS, upward slanting cross hatches) and 3¢ untranslated region (UTS, wavy lines) were unchanged callus of the genotype HiII using microprojectile bombardment (Frame et al 2000). Herbicideresistant callus lines carrying the transgene were identified by PCR and were regenerated to plants.…”
Section: Development Of Transgenic Plants Expressing 1dx5 Using the Mmentioning
confidence: 99%