In this study, biodiesel fuel produced from crambe abyssinica plant using KOH and NaOH catalysts was mixed with standard diesel fuel and the effects on engine performance, combustion and emission were experimentally investigated. During the experiment, in-cylinder pressure data were specified for each test fuel and engine load. In addition, measurements of HC, NOx, CO and smoke emissions were carried out. With the obtained experimental data, parameters such as heat release rate, combustion stages, thermal efficiency, indicated mean effective pressure (imep), ignition delay, ringing intensity and specific fuel consumptions were calculated and evaluated in MATLAB/Simulink environment. It was concluded that the highest thermal efficiency values were achieved with CAKB25 mixed fuel under all engine load conditions. It has been determined that using crambe abyssinica KOH catalyst (CAK) and crambe abyssinica NaOH catalyst (CAN) biodiesel fuel mixtures on diesel engine instead of standard diesel fuel improves CO, HC, and smoke emissions but increases NOx values slightly.