Production performances and antioxidant activities of laying hens fed Aspergillus oryzae and phytase co-fermented wheat bran

Abstract

Objective: Wheat bran (WB) was co-fermented with Aspergillus oryzae and phytase (Phy) to determine whether co-fermentation improve WB phosphorus and fiber utilization in Isa-brown layers.Methods: A total of 112 Isa brown layer were randomly divided into 7 treatments with 8 replicates per a treatment and 2 hens per a replicate. The treatments included basal diet (control), basal diet supplemented with 250 unit/kg Phy (control+Phy), diet with 10% WB (10% WB), diet with 5% WB and 250 unit/kg Phy (5% WB+Phy) diet with 10% WB and 250 unit/kg Phy (10% WB+Phy), diet with 5% fermented WB supplemented with molasses and phy (PCFWH) and 125 unit/kg Phy (5% PCFWH), and diet with 10% PCFWH (10% PCFWH). The intestinal microbial population, intestinal morphology, serum antioxidant enzyme activities, and excreta phosphorus content were assessed.Results: In PCFWH, spore counts, protease activity, xylanase activity, and ferulic acid were 8.50 log/g dry matter (DM), 190 unit/g DM, 120 unit/g DM, and 127 μg/g, respectively. Xylobiose and xylotriose were released in PCFWH, while they were not detectable in WB. Antioxidant capacity was also enhanced in PCFWH compared to WB. The 10% WB+Phy and 10% PCFWH groups produced higher egg mass, but hens fed 5% WB+Phy had the lowest amount of feed intake. Eggs from 10% PCFWH had better eggshell weight, eggshell strength, and eggshell thickness. Birds fed with 10% PCFWH also had higher serum superoxide dismutase and catalase activities. Compare to control, 10% PCFWH significantly reduced excreta phosphorus content.Conclusion: Diet inclusion of 10% PCFWH improved egg quality, antioxidant status, and excreta phosphorus content of laying hens.

The Potential Utilization of High-Fiber Agricultural By-Products as Monogastric Animal Feed and Feed Additives: A Review

“…Phosphoric acid on inositol can combine with minerals or amino acids of valence 2 or 3 and reduce the absorption rate of nutrients and enzyme activity of animals [49][50][51][52]. In addition, the involvement of too much phytic acid may also lead to poor phosphorus utilization by animals, causing the accumulation of phosphorus in stool and this may cause pollution to the environment [53]. Fortunately, phytic acid can be degraded by phytase.…”

“…and Bacillus subtilis [99]. After fermentation, cellulose and lignin content decrease, while hemicellulose and extractable functional metabolites increase slightly [3,53]. However, other crude ingredients, such as crude protein and minerals, are concentrated [53].…”

“…After fermentation, cellulose and lignin content decrease, while hemicellulose and extractable functional metabolites increase slightly [3,53]. However, other crude ingredients, such as crude protein and minerals, are concentrated [53]. After probiotic fermentation, the anti-nutritional factors usually present in plants also decrease [97].…”

Effects of tea residues-fermented feed on production performance, egg quality, antioxidant capacity, caecal microbiota, and ammonia emissions of laying hens

“…Probiotics such as Lactobacillus, Saccharomyces, and Bacillus subtilis have been widely used in feed fermentation, but it has been shown that the amount of protease secreted by the strains through simple microbial fermentation alone is low and cannot meet the actual production demand (7). Bacterial and enzyme co-fermented feed refers to the addition of a certain amount of exogenous compound enzymes and probiotics for joint fermentation, thus degrading the anti-nutritional ingredients in the feed, regulating the bitterness of the feed, improving the palatability of the feed, compensating for the lack of enzyme production and poor enzymatic taste of a single microbial fermentation, promoting animal feeding, improving feed utilization and nutritional value (8)(9)(10).…”

Effect of fermented heat-treated rice bran on performance and possible role of intestinal microbiota in laying hens

“…Fermented feed can fully utilize unconventional feed to compensate for feed material shortages and enhance the culture environment, both of which are crucial for the breeding of laying hens without the use of antibiotics. Fermented feed has a certain favorable impact on the intestinal flora and host health ( Guo et al, 2021 ), increases the amount of lactic acid bacteria and the concentration of SCFAs in feces, improves intestinal digestive and metabolic activities, promotes antioxidant and anti-inflammatory effects, and enhances the production performance and egg quality of laying hens ( Huang et al, 2020 ; Li et al, 2020 ; Zhu et al, 2022 ). The gastrointestinal tract is a complex environment ( Kelly and Conway, 2001 ; Wei et al, 2013 ), and both the feeding environment and its health status affect the composition of intestinal microbes ( Wilson et al, 2005 ).…”