Products of <i>in Vivo</i> Peroxidation Are Present in Tissues of Vitamin E‐Deficient Rats and Dogs

Dratz, Edward A.; Farnsworth, Christopher C.; Loew, E.; Stephens, Robert J.; Thomas, David William; Van, Frederik J. G. M.

doi:10.1111/j.1749-6632.1989.tb14907.x

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…Elevated levels of HNE were found in the retina in vitamin E-deficient rats and dogs (8). In vitro oxidation of Peak Area/mg Creatinine vitamin E-depleted human low density lipoproteins yielded butanal, propanal, hexanal, 2,4-heptadienal, HHE, HNE, and HOE (36).…”

Section: Resultsmentioning

confidence: 99%

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Response of urinary lipophilic aldehydes and related carbonyl compounds to factors that stimulate lipid peroxidation in vivo

Csallany

Kim

Gallaher

2000

Lipids

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Peroxidation of lipids results in the formation of a number of aldehydic and other carbonyl-containing secondary degradation products. The effect of peroxidative stimuli mediated by vitamin E deficiency, a diet high in polyunsaturated fatty acids (containing cod liver oil), and carbon tetrachloride administration on urinary excretion of a number of lipophilic aldehydes and related carbonyl compounds was examined in rats. These secondary lipid peroxidation products were measured as 2,4-dinitrophenylhydrazine derivatives. All three treatments increased urinary excretion of secondary lipid peroxidation products, although the pattern of excretion of these products varied somewhat among the treatments. Significant increases were found in butanal, hexanal, octanal, butan-2-one, pentan-2-one, hex-2-enal, hepta-2,4-dienal, 4-hydroxyhex-2-enal, 4-hydroxyoct-2-enal, 4-hydroxynon-2-enal, and a number of unidentified carbonyl compounds. These results suggest that urinary excretion of these lipophilic secondary lipid peroxidation products is a useful and noninvasive marker of whole-body lipid peroxidation.

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Section: Resultsmentioning

confidence: 99%

“…The antioxidant activity of this vitamin is related to its scavenging action of peroxyl radicals by hydrogen donation. A number of in vivo and in vitro studies have shown that vitamin E deficiency results in lipid peroxidation and the formation of fatty acid peroxides and a number of secondary aldehydic degradation products (5)(6)(7)(8).…”

mentioning

confidence: 99%

Response of urinary lipophilic aldehydes and related carbonyl compounds to factors that stimulate lipid peroxidation in vivo

Csallany

Kim

Gallaher

2000

Lipids

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“…However, to prove that lipid peroxidation products are formed in retinas of vitamin Edeficient rats, gas chromatography-mass spectrometry (GC-MS) assays were developed and employed (9)(10)(11)(12). Using these specific and sensitive methods, it was possible to show the presence ofoxidized fatty acids and 4-hydroxyalkenals in rat retinas (13)(14)(15). The levels of these lipid peroxidation products were higher in vitamin E-deficient as compared to vitamin E-supplemented retinas, giving more support for the involvement of lipid peroxidation in retinal degeneration (13,14), although firm evidence is not yet available on the effect of light.…”

Section: Retina Degenerationmentioning

confidence: 99%

“…It was found that these products of lipid peroxidation are present in vitamin E-deficient and -supple-*Department of Chemistry and Biochemistry, 108 Gaines Hall, Montana State University, Bozeman, MT 59717. mented rat retinal tissues (13)(14)(15). Oxidized fatty acids in membrane phospholipids significantly alter the permeability of cell membranes, which may contribute to malfunction of cells and eventually to cell death (16).…”

Section: Dermentioning

confidence: 99%

Effects of ultraviolet light on the eye: role of protective glasses.

Kuijk

1991

Environ Health Perspect

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Global atmospheric changes such as depletion of ozone in the stratosphere are thought to lead to increased levels of ultraviolet radiation on earth. This can have adverse effects on human health, and long-term effects of ultraviolet light on the eye are of increasing concern. Ultraviolet light exposure to the eye has been associated with cataract formation and retinal degeneration. In both cases, it is hypothesized that ultraviolet light can initiate formation of free radicals, which can cause protein modification and lipid peroxidation. Several procedures can be recommended to prevent ultraviolet light damage to the eye, such as the use of suitable protective glasses when outdoors.

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“…It is shown that both in vivo [1,12] and in vitro [3,13] FRO modifies physicochemical properties of cell membranes and, consequently, impairs their functions, leading to the development of various pathologies [8,13].…”

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confidence: 99%

Free-radial oxidation of lipids in human serum as a function of vitamin E content

1997

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The relationship between free-radial oxidation of human serum lipids and serum content of vitamin E is studied by the chemiluminescence method. A linearity between chemiluminescence and vitamin E content is established. By approximating experhnental data we deduced a set of equations characterizing the chemiluminescence parameters as a function of vitamin E content. The correlation coefficients have been calculated in the 0:7605-0.9671 range.Key Words: chemiluminescence; vitamin E; free radicals; antioxidants; lipid peroxidation Free-radical oxidation (FRO) of lipids has been extensively studied. It is shown that both in vivo [1,12] and in vitro [3,13] FRO modifies physicochemical properties of cell membranes and, consequently, impairs their functions, leading to the development of various pathologies [8,13].Previously, we evaluated the role of synthetic and naturally occurring antioxidants (AO) in FRO of individual lipids and blood lipids in vitro [3] as well as the role of vitamin E in pathological processes [6]. However, there is no information regarding the relationship between the intensity of FRO of serum lipids and the content of vitamin E, the major fatsoluble serum AO [11], in healthy subjects.Our goal was to study the kinetics of FRO of serum lipids as a function of vitamin E content in healthy men using the chemiluminescence (CL) method. MATERIALS AND METHODSSera from 24 healthy male donors aged 28-48 years were used. Blood was collected from the cubital vein [5], ratio of Im~ • to the time, during which CL intensity decreases 2-fold (T) [7], and the tangent of the descending segment of the kinetic curve (tgc 0.Chemiluminescence was measured in a PKhL-01 device equipped with a FEU 84-3 photomultiplying unit and calibrated in absolute units quantum/ secX47t. Kinetic curves were recorded and processed with an AkhLG-2-01 analyzer using specially designed software. Serum concentration of vitanain E was determined as described elsewhere [10].Vitamin E concentration and CL parameters were measured randomly. In each experiment, the mean of three determinations was calculated. Correlations were calculated by approximation using the least squares method. RESULTSThe kinetics of serum CL recorded in the presence of hydrogen peroxide agrees with the literature data [5,8]. The fast flash of CL reaches the lnaximum that

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Products of in Vivo Peroxidation Are Present in Tissues of Vitamin E‐Deficient Rats and Dogs

Cited by 10 publications

References 18 publications

Response of urinary lipophilic aldehydes and related carbonyl compounds to factors that stimulate lipid peroxidation in vivo

Response of urinary lipophilic aldehydes and related carbonyl compounds to factors that stimulate lipid peroxidation in vivo

Effects of ultraviolet light on the eye: role of protective glasses.

Free-radial oxidation of lipids in human serum as a function of vitamin E content

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