Produksi Mesenchymal Stem Cell (MSC) dari Sumsum Tulang Belakang Mencit

Rinendyaputri, Ratih; Noviantari, Ariyani

doi:10.22435/jbmi.v4i1.4211.33-41

Cited by 7 publications

(9 citation statements)

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“…C). According to Rinendyaputri and Noviantari (2015), these are characteristics of mesenchymal stem-like cells. Round cells with a larger diameter than the first day of culture were also observed.…”

Section: The Morphology Of Mesenchymal Stem-like Cellmentioning

confidence: 99%

“…Round cells with a larger diameter than the first day of culture were also observed. The round cells had completed their mitotic division (Rinendyaputri and Noviantari, 2015). On the eighth day, the cells had changed into a fusiform shape, i.e., the cells looked elongated, formed many extensions, and were attached to the base of the petri dish (Figure 2).…”

Section: The Morphology Of Mesenchymal Stem-like Cellmentioning

confidence: 99%

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The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

2022

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Physalis minimahas been used as herbal medicine because it is believed by the community to cure neurodegenerative and cardiovascular diseases. This study aims to determine the effectiveness of P.minima extract in increasing the proliferation of mesenchymal stem cells (MSC) from mouse (Musmusculus) bone marrow (BM). BM from the femur and tibia were isolated using a flushing method. BM-MSC primary culture was conducted in mesenPROÒ medium at 37ᵒC in a 5% CO2 incubator until it reached a 70% confluence.BM-MSCs were sub-cultured overnight in Dulbecco's Modified Eagle's Media (mDMEM). The mDMEM was replaced with a treatment medium on the second day of subculture. The treatment medium was changed every three days and evaluated under an inverted microscope by counting the number of cells at the beginning and the end of the incubation period. The proliferation rate is expressed as PDT, which was statistically analyzed using ANOVA at a significance level of 0.05% and followed-up with Duncan's test. Statistically, P. minima leaf extract could significantly reduce the PDT value. The optimum dose of P. minima leaf extract that can increase the proliferation of BM-MSC was 0.8 mg/ml. It is concluded that P. minima leaf extract was effective as an inducer of BM-MSC proliferation. The data obtained is the preliminary data on the use of P.minimaextracts in stem cell-based therapy. The results of this study provide important information in scientifically proving the potential of P. minima extract on stem cell proliferation.

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Section: The Morphology Of Mesenchymal Stem-like Cellmentioning

confidence: 99%

Section: The Morphology Of Mesenchymal Stem-like Cellmentioning

confidence: 99%

The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

2022

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“…MSCs were isolated from the femur and tibia bones by following a modification of the method of Rinendyaputri et al (2015) [12] by…”

Section: Isolation Culture and Characterization Of Mscs From Rat Bomentioning

confidence: 99%

The Expression of Nestin in the Induced Differentiation Into Neurons of Rat Bone Marrow Mesenchymal Stem Cells by Neurotrophin-3 (Nt-3)

Noviantari

Antarianto²,

Rif’ati

et al. 2020

Int J App Pharm

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Objective: The aim of this study was to examine the role of NT-3 as a single neurotrophic factor in the expression of nestin in the neural differentiation of MSCs. Methods: MSCs were isolated from rat bone marrow and induced with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 and 14 d (the control was no NT-3). Nestin underwent immunocytochemical analysis on days 7 and 14. Five high-power random fields were documented. Results: A post-hoc analysis using LSD after one-way ANOVA test yielded a statistically significant difference in the percentage of nestin-positive cells in MSCs with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 d compared to the control group (p<0.05). The percentages of nestin-positive cells at concentrations of 20, 25, and 30 ng/ml, and in the control data on day 7 were 14.55±1.26%, 16.20±1.07%, 13.78±1.19%, and 9.81±0.79%, respectively. NT-3 at 25 ng/ml induced the highest MSCs neural differentiation on day 7 and remained constant until day 14. Conclusion: NT-3 plays a role in the early stage of differentiating MSCs from rat bone marrow into neurons, with the optimal concentration being 25 ng/ml.

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“…This procedure has been approved by the ethical committee of NIHRD. Isolation of MSCs from the femur and tibia bones of rat (rat bone marrow MSCs, rBMMSCs) was done by cutting off each bone and flushing modification methods by a modified method of Rinendyaputri and Noviantari (2015), by flushing the bones with a syringe of MEM cul ture medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Gibco), sodium bicarbonate (Sigma), non essential amino acids 1% (Sigma), mercaptoethanol 0.1 mM (Sigma), and gentamicin (Sigma). The cells were in cubated in a 5% CO 2 incubator (Heracell Vios 160i) at 37°C…”

Section: Isolation and Culture Of Rat Mscs From Bone Marrow And Adipomentioning

confidence: 99%

Differentiation ability of rat‐mesenchymal stem cells from bone marrow and adipose tissue to neurons and glial cells

Noviantari

Rinendyaputri

Ariyanto

2020

Indones. J. Biotechnol.

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Mesenchymal stem cells (MSCs) are multipotent cells and can differentiate into neurons and glial cells. In vitro differentiation would be done by the addition of various factors. There remains no comparison for the differentiation of MSCs from rat bone marrow (rBMMSCs) and adipose tissue (rATMSCS) into neurons and glial cells with basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and brain‐derived neurotrophic factor (BDNF). The aims of this study were to investigate the effect of bFGF, EGF, and BDNF supplementation on the differentiation ability of rBMMSCs and rATMSCs into neurons and glial cells. MSCs were cultured with bFGF and EGF for 4 days and then BDNF was added until day 8. Characterization of MSCs before and after induction was carried out by observing the cell morphology and several cell markers. Flowcytometry analysis was performed for MSCs markers (CD90, CD29) and neurons and glial cell markers (A2B5, Beta‐III‐tubulin, PSAN‐CAM); while MAP‐2, a neuron marker, was analyzed by immunocytochemistry. Induction of both types of MSCs showed MAP‐2‐positive cells, decreased MSCs markers, and in rBMMSCs showed increased neuron markers. The number of neuron marker positive cells in rBMMSCS was higher than rATMSCs. This study showed that the addition of bFGF, EGF, and BDNF to the medium induced rBMMSCs into neurons and glial cells, but the conditions were not optimal for rATMSC as judged by the expression of neural markers (A2B5, Beta‐III‐tubulin, PSAN‐CAM, and MAP‐2).

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Produksi Mesenchymal Stem Cell (MSC) dari Sumsum Tulang Belakang Mencit

Cited by 7 publications

References 0 publications

The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

The Expression of Nestin in the Induced Differentiation Into Neurons of Rat Bone Marrow Mesenchymal Stem Cells by Neurotrophin-3 (Nt-3)

Differentiation ability of rat‐mesenchymal stem cells from bone marrow and adipose tissue to neurons and glial cells

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