Profile of Enterobacteria Resistant to Beta-Lactams

Abstract

A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is performed with antibiotics, of which the most used are beta-lactams. The aim of this study was to correlate antimicrobial resistance profiles in Enterobacteriaceae by phenotypic methods and molecular identification of 14 beta-lactamase coding genes. In this study, 70 exclusive isolates from Brazil were used, half of which were collected in veterinary clinics or hospitals Phenotypic methodologies were used and real-time PCR was the molecular methodology used, through the Sybr Green system. Regargding the results found in the tests it was observed that 74.28% were resistant to ampicillin, 62.85% were resistant to amoxicillin associated with clavalunate. The mechanism of resistance that presented the highest expression was ESBL (17.14%). The genes studied that were detected in a greater number of species were blaGIM and blaSIM (66.66% of the samples) and the one that was amplified in a smaller number of samples was blaVIM (16.66%). Therefore, high and worrying levels of antimicrobial resistance have been found in enterobacteria, and a way to minimize the accelerated emergence of their resistance includes developing or improving techniques that generate diagnoses with high efficiency and speed.

In vitro biofabrication of silver nanoparticles: Optimization, characterization, and activity against beta-lactamases-resistant Enterococcus faecalis

“…The correlation between the production of -lactamases and the spread of resistance among isolates of Gram-positive pathogens is very high, forming serious clinical challenges (Jubeh et al 2020). In particular, ESBLs (extended-spectrum beta-lactamases) are enzymes that deactivate β-lactam antibiotics by hydrolysis and have the ability to transfer bacterial resistance to the penicillins, rst-, second-, and third-generation cephalosporins (Santos et al 2020). ESBL-encoding genes have been categorized into three main types: bla CTX−M , bla SHV and bla TEM (Ahmed et al 2021).…”

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Vantagens da utilização de antibióticos encapsulados em lipossomas para o combate de infecções causadas por enterobactérias

“…As enterobactérias são uma grande família de bactérias Gram-negativas, bacilos anaeróbicos facultativos não formadores de esporos, onde sua maioria é capaz de crescer em temperaturas entre 25 e 37 °C. Existem em torno de 53 gêneros de Enterobacteriaceae, dos quais mais de 170 espécies já foram nomeadas (Kang et al, 2018;Santos et al, 2020). Este grupo é amplamente encontrado na natureza como no solo, água, vegetais e na microbiota gastrointestinal de humanos e animais, onde a Escherichia coli é a espécie mais abundante da família.…”

“…Este grupo é amplamente encontrado na natureza como no solo, água, vegetais e na microbiota gastrointestinal de humanos e animais, onde a Escherichia coli é a espécie mais abundante da família. Dentre os 53 gêneros de Enterobacteriaceae, cerca de 26 gêneros foram associados a infecções em humanos (Linninge et al, 2018;Pati et al, 2017;Santos et al, 2020).…”